First isolation and characterization of Brucella microti from wild boar

Zsuzsanna Rónai, Zsuzsa Kreizinger, Ádám Dán, Kevin Drees, Jeffrey T Foster, Krisztián Bányai, Szilvia Marton, Levente Szeredi, Szilárd Jánosi, Miklós Gyuranecz

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Brucella microti was first isolated from common vole (Microtus arvalis) in the Czech Republic in Central Europe in 2007. As B. microti is the only Brucella species known to live in soil, its distribution, ecology, zoonotic potential, and genomic organization is of particular interest. The present paper is the first to report the isolation of B. microti from a wild boar (Sus scrofa), which is also the first isolation of this bacterial species in Hungary. Results: The B. microti isolate was cultured, after enrichment in Brucella-selective broth, from the submandibular lymph node of a female wild boar that was taken by hunters in Hungary near the Austrian border in September 2014. Histological and immunohistological examinations of the lymph node sections with B. abortus-, B. suis- and B. canis-specific sera gave negative results. The isolate did not require CO<inf>2</inf> for growth, was oxidase, catalase, and urease positive, H<inf>2</inf>S negative, grew well in the presence of 20 μg/ml basic fuchsin and thionin, and had brownish pigmentation after three days of incubation. It gave strong positive agglutination with anti-A and anti-M but had a negative reaction with anti-R monospecific sera. The API 20 NE test identified it as Ochrobactrum anthropi with 99.9 % identity, and it showed B. microti-specific banding pattern in the Bruce- and Suis-ladder multiplex PCR systems. Whole genome re-sequencing identified 30 SNPs in orthologous loci when compared to the B. microti reference genome available in GenBank, and the MLVA analysis yielded a unique profile. Conclusions: Given that the female wild boar did not develop any clinical disease, we hypothesize that this host species only harboured the bacterium, serving as a possible reservoir capable of maintaining and spreading this pathogen. The infectious source could have been either a rodent, a carcass that had been eaten or infection occurred via the boar rooting in soil. The low number of discovered SNPs suggests an unexpectedly high level of genetic homogeneity in this Brucella species.

Original languageEnglish (US)
Article number147
JournalBMC Veterinary Research
Volume11
Issue number1
DOIs
StatePublished - Jul 11 2015
Externally publishedYes

Fingerprint

Sus scrofa
Brucella
Arvicolinae
Microtus
wild boars
Microtus arvalis
Hungary
lymph nodes
Ochrobactrum anthropi
Single Nucleotide Polymorphism
ladders
Canis
Soil
genome
Thionins
Lymph Nodes
agglutination
Genome
urease
boars

Keywords

  • Biochemistry
  • Brucella microti
  • Hungary
  • Immunohistochemistry
  • MLVA
  • Morphology
  • Whole genome sequencing
  • Wild boar

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Rónai, Z., Kreizinger, Z., Dán, Á., Drees, K., Foster, J. T., Bányai, K., ... Gyuranecz, M. (2015). First isolation and characterization of Brucella microti from wild boar. BMC Veterinary Research, 11(1), [147]. https://doi.org/10.1186/s12917-015-0456-z

First isolation and characterization of Brucella microti from wild boar. / Rónai, Zsuzsanna; Kreizinger, Zsuzsa; Dán, Ádám; Drees, Kevin; Foster, Jeffrey T; Bányai, Krisztián; Marton, Szilvia; Szeredi, Levente; Jánosi, Szilárd; Gyuranecz, Miklós.

In: BMC Veterinary Research, Vol. 11, No. 1, 147, 11.07.2015.

Research output: Contribution to journalArticle

Rónai, Z, Kreizinger, Z, Dán, Á, Drees, K, Foster, JT, Bányai, K, Marton, S, Szeredi, L, Jánosi, S & Gyuranecz, M 2015, 'First isolation and characterization of Brucella microti from wild boar', BMC Veterinary Research, vol. 11, no. 1, 147. https://doi.org/10.1186/s12917-015-0456-z
Rónai, Zsuzsanna ; Kreizinger, Zsuzsa ; Dán, Ádám ; Drees, Kevin ; Foster, Jeffrey T ; Bányai, Krisztián ; Marton, Szilvia ; Szeredi, Levente ; Jánosi, Szilárd ; Gyuranecz, Miklós. / First isolation and characterization of Brucella microti from wild boar. In: BMC Veterinary Research. 2015 ; Vol. 11, No. 1.
@article{6aeecd76d63a4be8baa2a9eecb839132,
title = "First isolation and characterization of Brucella microti from wild boar",
abstract = "Background: Brucella microti was first isolated from common vole (Microtus arvalis) in the Czech Republic in Central Europe in 2007. As B. microti is the only Brucella species known to live in soil, its distribution, ecology, zoonotic potential, and genomic organization is of particular interest. The present paper is the first to report the isolation of B. microti from a wild boar (Sus scrofa), which is also the first isolation of this bacterial species in Hungary. Results: The B. microti isolate was cultured, after enrichment in Brucella-selective broth, from the submandibular lymph node of a female wild boar that was taken by hunters in Hungary near the Austrian border in September 2014. Histological and immunohistological examinations of the lymph node sections with B. abortus-, B. suis- and B. canis-specific sera gave negative results. The isolate did not require CO2 for growth, was oxidase, catalase, and urease positive, H2S negative, grew well in the presence of 20 μg/ml basic fuchsin and thionin, and had brownish pigmentation after three days of incubation. It gave strong positive agglutination with anti-A and anti-M but had a negative reaction with anti-R monospecific sera. The API 20 NE test identified it as Ochrobactrum anthropi with 99.9 {\%} identity, and it showed B. microti-specific banding pattern in the Bruce- and Suis-ladder multiplex PCR systems. Whole genome re-sequencing identified 30 SNPs in orthologous loci when compared to the B. microti reference genome available in GenBank, and the MLVA analysis yielded a unique profile. Conclusions: Given that the female wild boar did not develop any clinical disease, we hypothesize that this host species only harboured the bacterium, serving as a possible reservoir capable of maintaining and spreading this pathogen. The infectious source could have been either a rodent, a carcass that had been eaten or infection occurred via the boar rooting in soil. The low number of discovered SNPs suggests an unexpectedly high level of genetic homogeneity in this Brucella species.",
keywords = "Biochemistry, Brucella microti, Hungary, Immunohistochemistry, MLVA, Morphology, Whole genome sequencing, Wild boar",
author = "Zsuzsanna R{\'o}nai and Zsuzsa Kreizinger and {\'A}d{\'a}m D{\'a}n and Kevin Drees and Foster, {Jeffrey T} and Kriszti{\'a}n B{\'a}nyai and Szilvia Marton and Levente Szeredi and Szil{\'a}rd J{\'a}nosi and Mikl{\'o}s Gyuranecz",
year = "2015",
month = "7",
day = "11",
doi = "10.1186/s12917-015-0456-z",
language = "English (US)",
volume = "11",
journal = "BMC Veterinary Research",
issn = "1746-6148",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - First isolation and characterization of Brucella microti from wild boar

AU - Rónai, Zsuzsanna

AU - Kreizinger, Zsuzsa

AU - Dán, Ádám

AU - Drees, Kevin

AU - Foster, Jeffrey T

AU - Bányai, Krisztián

AU - Marton, Szilvia

AU - Szeredi, Levente

AU - Jánosi, Szilárd

AU - Gyuranecz, Miklós

PY - 2015/7/11

Y1 - 2015/7/11

N2 - Background: Brucella microti was first isolated from common vole (Microtus arvalis) in the Czech Republic in Central Europe in 2007. As B. microti is the only Brucella species known to live in soil, its distribution, ecology, zoonotic potential, and genomic organization is of particular interest. The present paper is the first to report the isolation of B. microti from a wild boar (Sus scrofa), which is also the first isolation of this bacterial species in Hungary. Results: The B. microti isolate was cultured, after enrichment in Brucella-selective broth, from the submandibular lymph node of a female wild boar that was taken by hunters in Hungary near the Austrian border in September 2014. Histological and immunohistological examinations of the lymph node sections with B. abortus-, B. suis- and B. canis-specific sera gave negative results. The isolate did not require CO2 for growth, was oxidase, catalase, and urease positive, H2S negative, grew well in the presence of 20 μg/ml basic fuchsin and thionin, and had brownish pigmentation after three days of incubation. It gave strong positive agglutination with anti-A and anti-M but had a negative reaction with anti-R monospecific sera. The API 20 NE test identified it as Ochrobactrum anthropi with 99.9 % identity, and it showed B. microti-specific banding pattern in the Bruce- and Suis-ladder multiplex PCR systems. Whole genome re-sequencing identified 30 SNPs in orthologous loci when compared to the B. microti reference genome available in GenBank, and the MLVA analysis yielded a unique profile. Conclusions: Given that the female wild boar did not develop any clinical disease, we hypothesize that this host species only harboured the bacterium, serving as a possible reservoir capable of maintaining and spreading this pathogen. The infectious source could have been either a rodent, a carcass that had been eaten or infection occurred via the boar rooting in soil. The low number of discovered SNPs suggests an unexpectedly high level of genetic homogeneity in this Brucella species.

AB - Background: Brucella microti was first isolated from common vole (Microtus arvalis) in the Czech Republic in Central Europe in 2007. As B. microti is the only Brucella species known to live in soil, its distribution, ecology, zoonotic potential, and genomic organization is of particular interest. The present paper is the first to report the isolation of B. microti from a wild boar (Sus scrofa), which is also the first isolation of this bacterial species in Hungary. Results: The B. microti isolate was cultured, after enrichment in Brucella-selective broth, from the submandibular lymph node of a female wild boar that was taken by hunters in Hungary near the Austrian border in September 2014. Histological and immunohistological examinations of the lymph node sections with B. abortus-, B. suis- and B. canis-specific sera gave negative results. The isolate did not require CO2 for growth, was oxidase, catalase, and urease positive, H2S negative, grew well in the presence of 20 μg/ml basic fuchsin and thionin, and had brownish pigmentation after three days of incubation. It gave strong positive agglutination with anti-A and anti-M but had a negative reaction with anti-R monospecific sera. The API 20 NE test identified it as Ochrobactrum anthropi with 99.9 % identity, and it showed B. microti-specific banding pattern in the Bruce- and Suis-ladder multiplex PCR systems. Whole genome re-sequencing identified 30 SNPs in orthologous loci when compared to the B. microti reference genome available in GenBank, and the MLVA analysis yielded a unique profile. Conclusions: Given that the female wild boar did not develop any clinical disease, we hypothesize that this host species only harboured the bacterium, serving as a possible reservoir capable of maintaining and spreading this pathogen. The infectious source could have been either a rodent, a carcass that had been eaten or infection occurred via the boar rooting in soil. The low number of discovered SNPs suggests an unexpectedly high level of genetic homogeneity in this Brucella species.

KW - Biochemistry

KW - Brucella microti

KW - Hungary

KW - Immunohistochemistry

KW - MLVA

KW - Morphology

KW - Whole genome sequencing

KW - Wild boar

UR - http://www.scopus.com/inward/record.url?scp=84936859094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84936859094&partnerID=8YFLogxK

U2 - 10.1186/s12917-015-0456-z

DO - 10.1186/s12917-015-0456-z

M3 - Article

C2 - 26163135

AN - SCOPUS:84936863914

VL - 11

JO - BMC Veterinary Research

JF - BMC Veterinary Research

SN - 1746-6148

IS - 1

M1 - 147

ER -