Fiber supplementation influences phylogenetic structure and functional capacity of the human intestinal microbiome: Follow-up of a randomized controlled trial

Hannah D. Holscher, James G Caporaso, Seema Hooda, Jennifer M. Brulc, George C. Fahey, Kelly S. Swanson

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Background: In our published randomized, double-blind, placebocontrolled, 3-period crossover trial, healthy adult men ( n = 21) consumed bars containing no supplemental fiber (placebo; NFC), polydextrose (21 g/d), and soluble corn fiber (SCF; 21 g/d) for 21 d each. Fecal specimens were collected between days 16 and 21 for fermentative end-product analysis and 16S ribosomal RNA bacterial gene amplification for bacterial taxa identification. Fiber supplementation decreased fecal putrefaction compounds and shifted abundances of several bacterial taxa. Objective: The objective was to perform whole-genome shotgun 454 pyrosequencing on the same fecal specimens collected in that clinical trial to obtain comprehensive fecal bacterial genome sequencing coverage and explore the full range of bacterial genetic information in the fecal microbiome, thereby using a systematic approach to study the impact of dietary fiber supplementation on fecal metabolites, bacterial taxa, and bacterial metagenomes. Design: Fecal samples were subjected to whole-genome shotgun 454 pyrosequencing to identify both fecal bacterial populations present and their functional genetic capacity. Results: Whole-genome shotgun sequencing results revealed that fiber consumption shifted the Bacteroidetes:Firmicutes ratio, increasing the relative abundance of Bacteroidetes 12 ± 2% and 13 ± 2% with polydextrose and SCF, respectively, compared with NFC. Bivariate correlations showed a positive correlation between the Bacteroidetes: Firmicutes ratio and total dietary fiber intake but not body mass index. Principal coordinates analysis of Bray-Curtis distances indicated that bacterial gene composition was more similar in participants consuming fibers (polydextrose and SCF combined) in comparison with NFC. Shifts in bacterial gene abundances after polydextrose and SCF supplementation included genes associated with carbohydrate, amino acid, and lipid metabolism, as well as metabolism of cofactors and vitamins. Conclusion: This study conveys novel information about the impact of dietary fiber supplementation on the phylogenetic structure and functional capacity of the fecal microbiome of healthy adults.This trial was registered at clinical trials.gov as NCT02091349.

Original languageEnglish (US)
Pages (from-to)55-64
Number of pages10
JournalAmerican Journal of Clinical Nutrition
Volume101
Issue number1
DOIs
StatePublished - Jan 1 2015

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polydextrose
Microbiota
Bacteroidetes
Bacterial Genes
Firearms
Dietary Fiber
Randomized Controlled Trials
Genome
Dietary Supplements
Metagenome
Clinical Trials
16S Ribosomal RNA
Bacterial Genomes
Gene Amplification
rRNA Genes
Lipid Metabolism
Vitamins
Cross-Over Studies
Zea mays
Body Mass Index

Keywords

  • Fiber
  • Gastrointestinal microbiota
  • Metagenome
  • Microbiome
  • Prebiotic

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

Cite this

Fiber supplementation influences phylogenetic structure and functional capacity of the human intestinal microbiome : Follow-up of a randomized controlled trial. / Holscher, Hannah D.; Caporaso, James G; Hooda, Seema; Brulc, Jennifer M.; Fahey, George C.; Swanson, Kelly S.

In: American Journal of Clinical Nutrition, Vol. 101, No. 1, 01.01.2015, p. 55-64.

Research output: Contribution to journalArticle

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AU - Fahey, George C.

AU - Swanson, Kelly S.

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AB - Background: In our published randomized, double-blind, placebocontrolled, 3-period crossover trial, healthy adult men ( n = 21) consumed bars containing no supplemental fiber (placebo; NFC), polydextrose (21 g/d), and soluble corn fiber (SCF; 21 g/d) for 21 d each. Fecal specimens were collected between days 16 and 21 for fermentative end-product analysis and 16S ribosomal RNA bacterial gene amplification for bacterial taxa identification. Fiber supplementation decreased fecal putrefaction compounds and shifted abundances of several bacterial taxa. Objective: The objective was to perform whole-genome shotgun 454 pyrosequencing on the same fecal specimens collected in that clinical trial to obtain comprehensive fecal bacterial genome sequencing coverage and explore the full range of bacterial genetic information in the fecal microbiome, thereby using a systematic approach to study the impact of dietary fiber supplementation on fecal metabolites, bacterial taxa, and bacterial metagenomes. Design: Fecal samples were subjected to whole-genome shotgun 454 pyrosequencing to identify both fecal bacterial populations present and their functional genetic capacity. Results: Whole-genome shotgun sequencing results revealed that fiber consumption shifted the Bacteroidetes:Firmicutes ratio, increasing the relative abundance of Bacteroidetes 12 ± 2% and 13 ± 2% with polydextrose and SCF, respectively, compared with NFC. Bivariate correlations showed a positive correlation between the Bacteroidetes: Firmicutes ratio and total dietary fiber intake but not body mass index. Principal coordinates analysis of Bray-Curtis distances indicated that bacterial gene composition was more similar in participants consuming fibers (polydextrose and SCF combined) in comparison with NFC. Shifts in bacterial gene abundances after polydextrose and SCF supplementation included genes associated with carbohydrate, amino acid, and lipid metabolism, as well as metabolism of cofactors and vitamins. Conclusion: This study conveys novel information about the impact of dietary fiber supplementation on the phylogenetic structure and functional capacity of the fecal microbiome of healthy adults.This trial was registered at clinical trials.gov as NCT02091349.

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