Fiber size and myosin phenotypes of selected Rhesus hindlimb muscles after a 14-day spaceflight.

R. R. Roy, S. C. Bodine, David J Pierotti, J. A. Kim, R. J. Talmadge, G. Barkhoudarian, J. W. Fanton, I. Koslovskaya, V. R. Edgerton

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Open muscle biopsies were obtained from Rhesus soleus (slow ankle extensor), medial gastrocnemius (fast ankle extensor) and tibialis anterior (fast ankle flexor) muscles before and after either a 14-day spaceflight (BION 11, n=2) or ground-based flight simulation (n=3) and in time-matched controls (n=5). Fiber type distribution (immunohistochemistry), myosin heavy chain (MHC) composition (gel electrophoresis) and fiber size were determined. There was a large amount of inter-animal variability and there were no significant pre-post differences for any variable under any condition for any muscle studied. However, each muscle showed trends towards adaptation. Based on the immunohistochemical analyses, the percentage of type I fibers in the soleus was 68 and 86% in pre and 43 and 70% in post biopsies of the simulation and flight groups. The number of hybrid (containing both fast and slow MHC) fibers increased in both groups. MHC composition changed in a similar direction. Type I and hybrid fibers were 23 and 31% smaller after than before flight. In the medial gastrocnemius, type I fibers were 16, 14 and 32% smaller in post compared to pre biopsies in control, simulation and flight Rhesus. In the tibialis anterior, type I fibers were approximately 14% smaller in post- than pre-flight biopsies. As expected the soleus, a slow anti-gravity muscle, was most affected after 14 days of weightlessness. Further, slow fibers in each muscle were more responsive to microgravity than fast fibers. All changes, however, were smaller than those observed in rats after the same duration of flight. This differential effect may be related to the partial restraint of Rhesus in the chaired position compared to the free-floating position of rats in the cage and/or to differences in the contractile protein turnover rates between species.

Original languageEnglish (US)
Pages (from-to)55-62
Number of pages8
JournalJournal of gravitational physiology : a journal of the International Society for Gravitational Physiology
Volume6
Issue number2
StatePublished - 1999
Externally publishedYes

Fingerprint

Space Flight
Myosins
Hindlimb
Phenotype
Muscles
Myosin Heavy Chains
Ankle
Biopsy
Weightlessness
Contractile Proteins
Gravitation
Electrophoresis
Gels
Immunohistochemistry

Cite this

Fiber size and myosin phenotypes of selected Rhesus hindlimb muscles after a 14-day spaceflight. / Roy, R. R.; Bodine, S. C.; Pierotti, David J; Kim, J. A.; Talmadge, R. J.; Barkhoudarian, G.; Fanton, J. W.; Koslovskaya, I.; Edgerton, V. R.

In: Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology, Vol. 6, No. 2, 1999, p. 55-62.

Research output: Contribution to journalArticle

Roy, R. R. ; Bodine, S. C. ; Pierotti, David J ; Kim, J. A. ; Talmadge, R. J. ; Barkhoudarian, G. ; Fanton, J. W. ; Koslovskaya, I. ; Edgerton, V. R. / Fiber size and myosin phenotypes of selected Rhesus hindlimb muscles after a 14-day spaceflight. In: Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology. 1999 ; Vol. 6, No. 2. pp. 55-62.
@article{bb66f1bc12d94f4485ccd3c05e9d427e,
title = "Fiber size and myosin phenotypes of selected Rhesus hindlimb muscles after a 14-day spaceflight.",
abstract = "Open muscle biopsies were obtained from Rhesus soleus (slow ankle extensor), medial gastrocnemius (fast ankle extensor) and tibialis anterior (fast ankle flexor) muscles before and after either a 14-day spaceflight (BION 11, n=2) or ground-based flight simulation (n=3) and in time-matched controls (n=5). Fiber type distribution (immunohistochemistry), myosin heavy chain (MHC) composition (gel electrophoresis) and fiber size were determined. There was a large amount of inter-animal variability and there were no significant pre-post differences for any variable under any condition for any muscle studied. However, each muscle showed trends towards adaptation. Based on the immunohistochemical analyses, the percentage of type I fibers in the soleus was 68 and 86{\%} in pre and 43 and 70{\%} in post biopsies of the simulation and flight groups. The number of hybrid (containing both fast and slow MHC) fibers increased in both groups. MHC composition changed in a similar direction. Type I and hybrid fibers were 23 and 31{\%} smaller after than before flight. In the medial gastrocnemius, type I fibers were 16, 14 and 32{\%} smaller in post compared to pre biopsies in control, simulation and flight Rhesus. In the tibialis anterior, type I fibers were approximately 14{\%} smaller in post- than pre-flight biopsies. As expected the soleus, a slow anti-gravity muscle, was most affected after 14 days of weightlessness. Further, slow fibers in each muscle were more responsive to microgravity than fast fibers. All changes, however, were smaller than those observed in rats after the same duration of flight. This differential effect may be related to the partial restraint of Rhesus in the chaired position compared to the free-floating position of rats in the cage and/or to differences in the contractile protein turnover rates between species.",
author = "Roy, {R. R.} and Bodine, {S. C.} and Pierotti, {David J} and Kim, {J. A.} and Talmadge, {R. J.} and G. Barkhoudarian and Fanton, {J. W.} and I. Koslovskaya and Edgerton, {V. R.}",
year = "1999",
language = "English (US)",
volume = "6",
pages = "55--62",
journal = "Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology",
issn = "1077-9248",
publisher = "Galileo Foundation",
number = "2",

}

TY - JOUR

T1 - Fiber size and myosin phenotypes of selected Rhesus hindlimb muscles after a 14-day spaceflight.

AU - Roy, R. R.

AU - Bodine, S. C.

AU - Pierotti, David J

AU - Kim, J. A.

AU - Talmadge, R. J.

AU - Barkhoudarian, G.

AU - Fanton, J. W.

AU - Koslovskaya, I.

AU - Edgerton, V. R.

PY - 1999

Y1 - 1999

N2 - Open muscle biopsies were obtained from Rhesus soleus (slow ankle extensor), medial gastrocnemius (fast ankle extensor) and tibialis anterior (fast ankle flexor) muscles before and after either a 14-day spaceflight (BION 11, n=2) or ground-based flight simulation (n=3) and in time-matched controls (n=5). Fiber type distribution (immunohistochemistry), myosin heavy chain (MHC) composition (gel electrophoresis) and fiber size were determined. There was a large amount of inter-animal variability and there were no significant pre-post differences for any variable under any condition for any muscle studied. However, each muscle showed trends towards adaptation. Based on the immunohistochemical analyses, the percentage of type I fibers in the soleus was 68 and 86% in pre and 43 and 70% in post biopsies of the simulation and flight groups. The number of hybrid (containing both fast and slow MHC) fibers increased in both groups. MHC composition changed in a similar direction. Type I and hybrid fibers were 23 and 31% smaller after than before flight. In the medial gastrocnemius, type I fibers were 16, 14 and 32% smaller in post compared to pre biopsies in control, simulation and flight Rhesus. In the tibialis anterior, type I fibers were approximately 14% smaller in post- than pre-flight biopsies. As expected the soleus, a slow anti-gravity muscle, was most affected after 14 days of weightlessness. Further, slow fibers in each muscle were more responsive to microgravity than fast fibers. All changes, however, were smaller than those observed in rats after the same duration of flight. This differential effect may be related to the partial restraint of Rhesus in the chaired position compared to the free-floating position of rats in the cage and/or to differences in the contractile protein turnover rates between species.

AB - Open muscle biopsies were obtained from Rhesus soleus (slow ankle extensor), medial gastrocnemius (fast ankle extensor) and tibialis anterior (fast ankle flexor) muscles before and after either a 14-day spaceflight (BION 11, n=2) or ground-based flight simulation (n=3) and in time-matched controls (n=5). Fiber type distribution (immunohistochemistry), myosin heavy chain (MHC) composition (gel electrophoresis) and fiber size were determined. There was a large amount of inter-animal variability and there were no significant pre-post differences for any variable under any condition for any muscle studied. However, each muscle showed trends towards adaptation. Based on the immunohistochemical analyses, the percentage of type I fibers in the soleus was 68 and 86% in pre and 43 and 70% in post biopsies of the simulation and flight groups. The number of hybrid (containing both fast and slow MHC) fibers increased in both groups. MHC composition changed in a similar direction. Type I and hybrid fibers were 23 and 31% smaller after than before flight. In the medial gastrocnemius, type I fibers were 16, 14 and 32% smaller in post compared to pre biopsies in control, simulation and flight Rhesus. In the tibialis anterior, type I fibers were approximately 14% smaller in post- than pre-flight biopsies. As expected the soleus, a slow anti-gravity muscle, was most affected after 14 days of weightlessness. Further, slow fibers in each muscle were more responsive to microgravity than fast fibers. All changes, however, were smaller than those observed in rats after the same duration of flight. This differential effect may be related to the partial restraint of Rhesus in the chaired position compared to the free-floating position of rats in the cage and/or to differences in the contractile protein turnover rates between species.

UR - http://www.scopus.com/inward/record.url?scp=0033205830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033205830&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 55

EP - 62

JO - Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology

JF - Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology

SN - 1077-9248

IS - 2

ER -