Exercise and exhaustion effects on glycogen synthesis pathways

Hans Gunderson, Nadja Wehmeyer, Diane Burnett, John Nauman, Cynthia J Hartzell, Scott Savage

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Female Sprague-Dawley rats were infused with [1-13C]glucose to measure the effect of endurance training and the effect of various metabolic conditions on pathways of hepatic glycogen synthesis. Four metabolic states [sedentary (S), trained (T), sedentary exhausted (SE), and trained exhausted (TE)] were studied. T and TE rats were trained on a motor-driven treadmill (30 m/min, 15% grade, 1.0 h/day, 5 days/wk) for 8-10 wk. After a 24-h fast, SE and TE rats were run to exhaustion (sedentary average = 78 min, trained average = 155 min) at a training pace and immediately infused with labeled glucose for 2 h. S and T rats were infused after a 24-h fast. After infusion, tissues were removed and glycogen was isolated and hydrolyzed to glucose. The glucose was measured for distribution of 13C by using nuclear magnetic resonance. Glycogen was synthesized predominantly by the indirect pathway for all metabolic states, indicating that infused glucose was first metabolized primarily in the peripheral tissue. The direct-pathway utilization was greater in rested S than in rested T animals (30 vs. 14%); however, for exhausted animals, the trained use of the direct pathway was greater (22 vs. 9%). Both TE and rested T animals utilize the indirect pathway a comparable amount. Sedentary animals, on the other hand, dramatically decreased utilization of the direct pathway, with exhaustive exercise changing from 30 to 9%. The results indicate that endurance training modifies glucose utilization during glycogen synthesis after fasting and exhaustive exercise.

Original languageEnglish (US)
Pages (from-to)2020-2026
Number of pages7
JournalJournal of Applied Physiology
Volume81
Issue number5
StatePublished - Nov 1996

Fingerprint

Glycogen
Glucose
Liver Glycogen
Metabolic Networks and Pathways
Sprague Dawley Rats
Fasting
Magnetic Resonance Spectroscopy

Keywords

  • carbon-13-nuclear magnetic resonance
  • endurance training
  • gluconeogenesis
  • glucose utilization
  • glycogenesis
  • glycolysis

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Gunderson, H., Wehmeyer, N., Burnett, D., Nauman, J., Hartzell, C. J., & Savage, S. (1996). Exercise and exhaustion effects on glycogen synthesis pathways. Journal of Applied Physiology, 81(5), 2020-2026.

Exercise and exhaustion effects on glycogen synthesis pathways. / Gunderson, Hans; Wehmeyer, Nadja; Burnett, Diane; Nauman, John; Hartzell, Cynthia J; Savage, Scott.

In: Journal of Applied Physiology, Vol. 81, No. 5, 11.1996, p. 2020-2026.

Research output: Contribution to journalArticle

Gunderson, H, Wehmeyer, N, Burnett, D, Nauman, J, Hartzell, CJ & Savage, S 1996, 'Exercise and exhaustion effects on glycogen synthesis pathways', Journal of Applied Physiology, vol. 81, no. 5, pp. 2020-2026.
Gunderson H, Wehmeyer N, Burnett D, Nauman J, Hartzell CJ, Savage S. Exercise and exhaustion effects on glycogen synthesis pathways. Journal of Applied Physiology. 1996 Nov;81(5):2020-2026.
Gunderson, Hans ; Wehmeyer, Nadja ; Burnett, Diane ; Nauman, John ; Hartzell, Cynthia J ; Savage, Scott. / Exercise and exhaustion effects on glycogen synthesis pathways. In: Journal of Applied Physiology. 1996 ; Vol. 81, No. 5. pp. 2020-2026.
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