Evidence for TREK-like tandem-pore domain channels in intrapulmonary chemoreceptor chemotransduction

Robert Wagner Bina, Steven C. Hempleman

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Intrapulmonary chemoreceptors (IPC) are carbon dioxide sensing neurons that innervate the lungs of birds, control breathing pattern, and are inhibited by halothane and intracellular acidosis. TASK and TREK are subfamilies of tandem-pore domain potassium leak channels, important in setting resting membrane potential, that are affected by volatile anesthetics and acidosis. We hypothesized that such channels might underlie signal transduction in IPC. We treated mallard ducks with four volatile anesthetics in increasing concentrations to test their effects on IPC discharge through single cell, extracellular recording from vagal fibers. Isoflurane inhalation attenuated IPC discharge only at 8.25% inspired (α = 0.05). Halothane attenuated IPC discharge significantly (α = 0.05) at all treatment levels. Chloroform at 3.8%, 5.6%, and 8.25% significantly attenuated IPC discharge (α = 0.05). Ether at 1.9%, 2.9%, and 3.8% significantly attenuated IPC discharge (α = 0.05), abolishing IPC discharge at 3.8% inspired. The pharmacological signature of IPC discharge attenuation suggests that IPC express tandem-pore domain leak channels similar to TREK channels, which are inhibited by intracellular acidosis.

Original languageEnglish (US)
Pages (from-to)120-131
Number of pages12
JournalRespiratory Physiology and Neurobiology
Volume156
Issue number2
DOIs
StatePublished - May 14 2007

Keywords

  • Carbon dioxide
  • Control of breathing
  • Potassium leak channels
  • Signal transduction
  • Volatile anesthesics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Pulmonary and Respiratory Medicine

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