Abstract
Do bacterial taxa demonstrate clear endemism, like macroorganisms, or can one site's bacterial community recapture the total phylogenetic diversity of the world's oceans? Here we compare a deep bacterial community characterization from one site in the English Channel (L4-DeepSeq) with 356 datasets from the International Census ofMarine Microbes (ICoMM) taken fromaround the globe (ranging frommarine pelagic and sediment samples to sponge-associated environments). At the L4-DeepSeq site, increasing sequencing depth uncovers greater phylogenetic overlap with the global ICoMMdata. This site contained 31.7-66.2% of operational taxonomic units identified in a given ICoMM biome. Extrapolation of this overlap suggests that 1.93 × 1011 sequences from the L4 site would capture all ICoMM bacterial phylogenetic diversity. Current technology trends suggest this limit may be attainable within 3 y. These results strongly suggest the marine biosphere maintains a previously undetected, persistent microbial seed bank.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4651-4655 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 110 |
| Issue number | 12 |
| DOIs | |
| State | Published - Mar 19 2013 |
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Keywords
- Deep sequencing
- Microbial ecology
- Rare biosphere
ASJC Scopus subject areas
- General
Cite this
Evidence for a persistent microbial seed bank throughout the global ocean. / Gibbons, Sean M.; Caporaso, James G; Pirrung, Meg; Field, Dawn; Knight, Rob; Gilbert, Jack A.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 12, 19.03.2013, p. 4651-4655.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence for a persistent microbial seed bank throughout the global ocean
AU - Gibbons, Sean M.
AU - Caporaso, James G
AU - Pirrung, Meg
AU - Field, Dawn
AU - Knight, Rob
AU - Gilbert, Jack A.
PY - 2013/3/19
Y1 - 2013/3/19
N2 - Do bacterial taxa demonstrate clear endemism, like macroorganisms, or can one site's bacterial community recapture the total phylogenetic diversity of the world's oceans? Here we compare a deep bacterial community characterization from one site in the English Channel (L4-DeepSeq) with 356 datasets from the International Census ofMarine Microbes (ICoMM) taken fromaround the globe (ranging frommarine pelagic and sediment samples to sponge-associated environments). At the L4-DeepSeq site, increasing sequencing depth uncovers greater phylogenetic overlap with the global ICoMMdata. This site contained 31.7-66.2% of operational taxonomic units identified in a given ICoMM biome. Extrapolation of this overlap suggests that 1.93 × 1011 sequences from the L4 site would capture all ICoMM bacterial phylogenetic diversity. Current technology trends suggest this limit may be attainable within 3 y. These results strongly suggest the marine biosphere maintains a previously undetected, persistent microbial seed bank.
AB - Do bacterial taxa demonstrate clear endemism, like macroorganisms, or can one site's bacterial community recapture the total phylogenetic diversity of the world's oceans? Here we compare a deep bacterial community characterization from one site in the English Channel (L4-DeepSeq) with 356 datasets from the International Census ofMarine Microbes (ICoMM) taken fromaround the globe (ranging frommarine pelagic and sediment samples to sponge-associated environments). At the L4-DeepSeq site, increasing sequencing depth uncovers greater phylogenetic overlap with the global ICoMMdata. This site contained 31.7-66.2% of operational taxonomic units identified in a given ICoMM biome. Extrapolation of this overlap suggests that 1.93 × 1011 sequences from the L4 site would capture all ICoMM bacterial phylogenetic diversity. Current technology trends suggest this limit may be attainable within 3 y. These results strongly suggest the marine biosphere maintains a previously undetected, persistent microbial seed bank.
KW - Deep sequencing
KW - Microbial ecology
KW - Rare biosphere
UR - http://www.scopus.com/inward/record.url?scp=84875249654&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875249654&partnerID=8YFLogxK
U2 - 10.1073/pnas.1217767110
DO - 10.1073/pnas.1217767110
M3 - Article
C2 - 23487761
AN - SCOPUS:84875249654
VL - 110
SP - 4651
EP - 4655
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 12
ER -