Evaluation of sequential coinfection with Anaplasma phagocytophilum and Anaplasma marginale in cattle

Bruce R. Hoar, Nathan C Nieto, Diane M. Rhodes, Janet E. Foley

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective - To determine whether sequelae of infection differed among single versus double infection with Anaplasma phagocytophilum or Anaplasma marginale, with and without tick salivary extract, in cattle. Animals - Eighteen 13-month old steers. Procedures - Treatment groups of 3 cattle each included A marginale inoculated ID followed on day 35 by A phagocytophilum without tick saliva, A phagocytophilum followed on day 10 by A marginale without tick saliva, A marginale followed on day 35 by A phagocytophilum with tick saliva, A phagocytophilum followed on day 10 by A marginale with tick saliva, tissue culture control injection, and tick saliva control injection. Infection was monitored via clinical observations, CBC, serologic testing, and PCR analysis of blood and tissues. Results - Infected cattle had significantly reduced weight gain. Anemia occurred 25 to 32 days after A marginale infection, which was attenuated by tick saliva. Parasitism was greater if cattle had not previously been inoculated with A phagocytophilum. Nine of the 12 treated cattle had positive results of PCR analysis for A phagocytophilum from at least 1 blood sample. Five tissue samples had positive results of PCR analysis for A phagocytophilum; PCR results for A marginale were positive in spleen, lung, lymph node, heart, and ear skin of infected cattle. Conclusions and Clinical Relevance - Results indicated an important biological interaction between A marginale and A phagocytophilum infection as well as with tick saliva in disease kinetics and severity in cattle, which may be important for interpreta of diagnostic tests and management of disease in areas where both pathogens occur.

Original languageEnglish (US)
Pages (from-to)1171-1178
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume69
Issue number9
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

Anaplasma marginale
Anaplasma phagocytophilum
Ticks
Coinfection
mixed infection
Saliva
saliva
ticks
cattle
Infection
Polymerase Chain Reaction
infection
Tick Control
Injections
injection
tissue analysis
Disease Management
hematologic tests
complications (disease)
Routine Diagnostic Tests

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of sequential coinfection with Anaplasma phagocytophilum and Anaplasma marginale in cattle. / Hoar, Bruce R.; Nieto, Nathan C; Rhodes, Diane M.; Foley, Janet E.

In: American Journal of Veterinary Research, Vol. 69, No. 9, 09.2008, p. 1171-1178.

Research output: Contribution to journalArticle

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