Evaluation of hepatic disease in mice following intradermal inoculation with Corynebacterium pseudotuberculosis

Nathan C Nieto, Janet E. Foley, N. James MacLachlan, Tom Yuan, Sharon J. Spier

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective - To evaluate clinical, microbiologic, and pathologic outcomes in mice after inoculation with 4 equine-origin Corynebacterium pseudotuberculosis strains. Animals - 15 C3H/HeJ mice. Procedures - In a preliminary study, the optimum route of inoculation was determined. In the main study, mice were allocated to 4 treatment groups (3 mice/group). One slow- or rapid-growing equine-origin C pseudotuberculosis strain was inoculated ID into the mice of each treatment group. Results - All 4 strains had distinct tropism for the liver. Histologic lesions associated with rapid-growing strains included focally extensive unencapsulated areas of acute, massive coagulative necrosis of hepatocytes with intralesional colonies of bacteria and variable portal hepatitis characterized by accumulations of mononuclear and polymorphonuclear inflammatory cells. In contrast, the livers of mice inoculated with slow-growing strains had multiple discrete, randomly distributed foci of hepatocellular necrosis and neutrophilic hepatitis that were considerably less severe than the lesions in the mice inoculated with the rapid-growing strains. Significantly more bacterial colonies were recovered from the organs of mice inoculated with rapid-growing than with slow-growing strains of bacteria. Bacteria were isolated from the liver, spleen, lungs, and mesenteric lymph nodes of mice inoculated with rapid-growing strains and from the liver and lymph nodes of mice inoculated with slow-growing strains. Conclusions and Clinical Relevance - Study of host-bacteria interactions in hosts that are naturally infected with C pseudotuberculosis is difficult because of underlying genetic variability among animals, expense, and requirements for multiple replicates and control animals. The C3H/HeJ mice may provide a useful means for studying virulence mechanisms of C pseudotuberculosis.

Original languageEnglish (US)
Pages (from-to)257-262
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume70
Issue number2
DOIs
StatePublished - Feb 2009
Externally publishedYes

Fingerprint

Corynebacterium pseudotuberculosis
liver diseases
Liver
mice
slow-growing strains
Bacteria
Inbred C3H Mouse
liver
Hepatitis
Horses
bacteria
hepatitis
Necrosis
lesions (animal)
Lymph Nodes
lymph nodes
necrosis
Tropism
horses
animals

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of hepatic disease in mice following intradermal inoculation with Corynebacterium pseudotuberculosis. / Nieto, Nathan C; Foley, Janet E.; MacLachlan, N. James; Yuan, Tom; Spier, Sharon J.

In: American Journal of Veterinary Research, Vol. 70, No. 2, 02.2009, p. 257-262.

Research output: Contribution to journalArticle

Nieto, Nathan C ; Foley, Janet E. ; MacLachlan, N. James ; Yuan, Tom ; Spier, Sharon J. / Evaluation of hepatic disease in mice following intradermal inoculation with Corynebacterium pseudotuberculosis. In: American Journal of Veterinary Research. 2009 ; Vol. 70, No. 2. pp. 257-262.
@article{ff7aa903cf6f4a07b82deebd5487cc6b,
title = "Evaluation of hepatic disease in mice following intradermal inoculation with Corynebacterium pseudotuberculosis",
abstract = "Objective - To evaluate clinical, microbiologic, and pathologic outcomes in mice after inoculation with 4 equine-origin Corynebacterium pseudotuberculosis strains. Animals - 15 C3H/HeJ mice. Procedures - In a preliminary study, the optimum route of inoculation was determined. In the main study, mice were allocated to 4 treatment groups (3 mice/group). One slow- or rapid-growing equine-origin C pseudotuberculosis strain was inoculated ID into the mice of each treatment group. Results - All 4 strains had distinct tropism for the liver. Histologic lesions associated with rapid-growing strains included focally extensive unencapsulated areas of acute, massive coagulative necrosis of hepatocytes with intralesional colonies of bacteria and variable portal hepatitis characterized by accumulations of mononuclear and polymorphonuclear inflammatory cells. In contrast, the livers of mice inoculated with slow-growing strains had multiple discrete, randomly distributed foci of hepatocellular necrosis and neutrophilic hepatitis that were considerably less severe than the lesions in the mice inoculated with the rapid-growing strains. Significantly more bacterial colonies were recovered from the organs of mice inoculated with rapid-growing than with slow-growing strains of bacteria. Bacteria were isolated from the liver, spleen, lungs, and mesenteric lymph nodes of mice inoculated with rapid-growing strains and from the liver and lymph nodes of mice inoculated with slow-growing strains. Conclusions and Clinical Relevance - Study of host-bacteria interactions in hosts that are naturally infected with C pseudotuberculosis is difficult because of underlying genetic variability among animals, expense, and requirements for multiple replicates and control animals. The C3H/HeJ mice may provide a useful means for studying virulence mechanisms of C pseudotuberculosis.",
author = "Nieto, {Nathan C} and Foley, {Janet E.} and MacLachlan, {N. James} and Tom Yuan and Spier, {Sharon J.}",
year = "2009",
month = "2",
doi = "10.2460/ajvr.70.2.257",
language = "English (US)",
volume = "70",
pages = "257--262",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "2",

}

TY - JOUR

T1 - Evaluation of hepatic disease in mice following intradermal inoculation with Corynebacterium pseudotuberculosis

AU - Nieto, Nathan C

AU - Foley, Janet E.

AU - MacLachlan, N. James

AU - Yuan, Tom

AU - Spier, Sharon J.

PY - 2009/2

Y1 - 2009/2

N2 - Objective - To evaluate clinical, microbiologic, and pathologic outcomes in mice after inoculation with 4 equine-origin Corynebacterium pseudotuberculosis strains. Animals - 15 C3H/HeJ mice. Procedures - In a preliminary study, the optimum route of inoculation was determined. In the main study, mice were allocated to 4 treatment groups (3 mice/group). One slow- or rapid-growing equine-origin C pseudotuberculosis strain was inoculated ID into the mice of each treatment group. Results - All 4 strains had distinct tropism for the liver. Histologic lesions associated with rapid-growing strains included focally extensive unencapsulated areas of acute, massive coagulative necrosis of hepatocytes with intralesional colonies of bacteria and variable portal hepatitis characterized by accumulations of mononuclear and polymorphonuclear inflammatory cells. In contrast, the livers of mice inoculated with slow-growing strains had multiple discrete, randomly distributed foci of hepatocellular necrosis and neutrophilic hepatitis that were considerably less severe than the lesions in the mice inoculated with the rapid-growing strains. Significantly more bacterial colonies were recovered from the organs of mice inoculated with rapid-growing than with slow-growing strains of bacteria. Bacteria were isolated from the liver, spleen, lungs, and mesenteric lymph nodes of mice inoculated with rapid-growing strains and from the liver and lymph nodes of mice inoculated with slow-growing strains. Conclusions and Clinical Relevance - Study of host-bacteria interactions in hosts that are naturally infected with C pseudotuberculosis is difficult because of underlying genetic variability among animals, expense, and requirements for multiple replicates and control animals. The C3H/HeJ mice may provide a useful means for studying virulence mechanisms of C pseudotuberculosis.

AB - Objective - To evaluate clinical, microbiologic, and pathologic outcomes in mice after inoculation with 4 equine-origin Corynebacterium pseudotuberculosis strains. Animals - 15 C3H/HeJ mice. Procedures - In a preliminary study, the optimum route of inoculation was determined. In the main study, mice were allocated to 4 treatment groups (3 mice/group). One slow- or rapid-growing equine-origin C pseudotuberculosis strain was inoculated ID into the mice of each treatment group. Results - All 4 strains had distinct tropism for the liver. Histologic lesions associated with rapid-growing strains included focally extensive unencapsulated areas of acute, massive coagulative necrosis of hepatocytes with intralesional colonies of bacteria and variable portal hepatitis characterized by accumulations of mononuclear and polymorphonuclear inflammatory cells. In contrast, the livers of mice inoculated with slow-growing strains had multiple discrete, randomly distributed foci of hepatocellular necrosis and neutrophilic hepatitis that were considerably less severe than the lesions in the mice inoculated with the rapid-growing strains. Significantly more bacterial colonies were recovered from the organs of mice inoculated with rapid-growing than with slow-growing strains of bacteria. Bacteria were isolated from the liver, spleen, lungs, and mesenteric lymph nodes of mice inoculated with rapid-growing strains and from the liver and lymph nodes of mice inoculated with slow-growing strains. Conclusions and Clinical Relevance - Study of host-bacteria interactions in hosts that are naturally infected with C pseudotuberculosis is difficult because of underlying genetic variability among animals, expense, and requirements for multiple replicates and control animals. The C3H/HeJ mice may provide a useful means for studying virulence mechanisms of C pseudotuberculosis.

UR - http://www.scopus.com/inward/record.url?scp=60749136617&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60749136617&partnerID=8YFLogxK

U2 - 10.2460/ajvr.70.2.257

DO - 10.2460/ajvr.70.2.257

M3 - Article

VL - 70

SP - 257

EP - 262

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 2

ER -