Evaluation of Anaplasma phagocytophilum infection in experimentally inoculated sheep and determination of Anaplasma spp seroprevalence in 8 free-ranging sheep flocks in California and Oregon

Jennifer K. Gorman, Bruce R. Hoar, Nathan C Nieto, Janet E. Foley

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3 Citations (Scopus)

Abstract

Objective-To evaluate disease progression in sheep experimentally inoculated with Anaplasma phagocytophilum and determine the Anaplasma spp seroprevalence in sheep in free-ranging flocks in the Sierra Nevada foothills and Oregon Coast Range. Animals-10 mature ewes seronegative for Anaplasma spp and 251 sheep from 8 flocks. Procedures-10 ewes received 1 of 3 treatments: A phagocytophilum Webster strain (n =4), A phagocytophilum MRK strain (4), or human promyelocytic leukemia cells (control treatment [2]). Sheep were monitored for signs of clinical disease, and blood samples were obtained for serologic and PCR assay evaluation intermittently for 48 days. From a subsample of sheep from each of 8 free-ranging flocks, blood samples were obtained to determine Anaplasma spp seroprevalence. Results-Sheep inoculated with A phagocytophilum developed subclinical or mild disease, whereas sheep inoculated with the control treatment did not develop any signs of disease. Only 2 ewes seroconverted; both had received the MRK strain. Anaplasma-specific DNA was detected in blood samples from 1 sheep in the Webster strain-inoculated group and 3 sheep in the MRK strain-inoculated group. Sheep seropositive for Anaplasma spp were detected in 5 of 8 flocks, and flocks in the Sierra Nevada foothills had higher within-flock seroprevalence (22%) than did flocks in the Oregon Coast Range (6.4%). Conclusions and Clinical Relevance-Infection with A phagocytophilum in mature sheep generally resulted in subclinical disease. Higher Anaplasma spp seroprevalence in sheep in the Sierra Nevada foothills corresponded to the geographic distribution of anaplasmosis reported for dogs, horses, and humans.

Original languageEnglish (US)
Pages (from-to)1029-1034
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume73
Issue number7
DOIs
StatePublished - Jul 2012
Externally publishedYes

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Anaplasma
Anaplasma phagocytophilum
Seroepidemiologic Studies
seroprevalence
Sheep
flocks
sheep
Infection
infection
ewes
blood
Sheep Diseases
Anaplasmosis
sheep diseases
coasts
anaplasmosis
Hematologic Diseases
leukemia
disease course
sampling

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{0a3a1e6709cd4bbdb8e16ac92a43e2ce,
title = "Evaluation of Anaplasma phagocytophilum infection in experimentally inoculated sheep and determination of Anaplasma spp seroprevalence in 8 free-ranging sheep flocks in California and Oregon",
abstract = "Objective-To evaluate disease progression in sheep experimentally inoculated with Anaplasma phagocytophilum and determine the Anaplasma spp seroprevalence in sheep in free-ranging flocks in the Sierra Nevada foothills and Oregon Coast Range. Animals-10 mature ewes seronegative for Anaplasma spp and 251 sheep from 8 flocks. Procedures-10 ewes received 1 of 3 treatments: A phagocytophilum Webster strain (n =4), A phagocytophilum MRK strain (4), or human promyelocytic leukemia cells (control treatment [2]). Sheep were monitored for signs of clinical disease, and blood samples were obtained for serologic and PCR assay evaluation intermittently for 48 days. From a subsample of sheep from each of 8 free-ranging flocks, blood samples were obtained to determine Anaplasma spp seroprevalence. Results-Sheep inoculated with A phagocytophilum developed subclinical or mild disease, whereas sheep inoculated with the control treatment did not develop any signs of disease. Only 2 ewes seroconverted; both had received the MRK strain. Anaplasma-specific DNA was detected in blood samples from 1 sheep in the Webster strain-inoculated group and 3 sheep in the MRK strain-inoculated group. Sheep seropositive for Anaplasma spp were detected in 5 of 8 flocks, and flocks in the Sierra Nevada foothills had higher within-flock seroprevalence (22{\%}) than did flocks in the Oregon Coast Range (6.4{\%}). Conclusions and Clinical Relevance-Infection with A phagocytophilum in mature sheep generally resulted in subclinical disease. Higher Anaplasma spp seroprevalence in sheep in the Sierra Nevada foothills corresponded to the geographic distribution of anaplasmosis reported for dogs, horses, and humans.",
author = "Gorman, {Jennifer K.} and Hoar, {Bruce R.} and Nieto, {Nathan C} and Foley, {Janet E.}",
year = "2012",
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doi = "10.2460/ajvr.73.7.1029",
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pages = "1029--1034",
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T1 - Evaluation of Anaplasma phagocytophilum infection in experimentally inoculated sheep and determination of Anaplasma spp seroprevalence in 8 free-ranging sheep flocks in California and Oregon

AU - Gorman, Jennifer K.

AU - Hoar, Bruce R.

AU - Nieto, Nathan C

AU - Foley, Janet E.

PY - 2012/7

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N2 - Objective-To evaluate disease progression in sheep experimentally inoculated with Anaplasma phagocytophilum and determine the Anaplasma spp seroprevalence in sheep in free-ranging flocks in the Sierra Nevada foothills and Oregon Coast Range. Animals-10 mature ewes seronegative for Anaplasma spp and 251 sheep from 8 flocks. Procedures-10 ewes received 1 of 3 treatments: A phagocytophilum Webster strain (n =4), A phagocytophilum MRK strain (4), or human promyelocytic leukemia cells (control treatment [2]). Sheep were monitored for signs of clinical disease, and blood samples were obtained for serologic and PCR assay evaluation intermittently for 48 days. From a subsample of sheep from each of 8 free-ranging flocks, blood samples were obtained to determine Anaplasma spp seroprevalence. Results-Sheep inoculated with A phagocytophilum developed subclinical or mild disease, whereas sheep inoculated with the control treatment did not develop any signs of disease. Only 2 ewes seroconverted; both had received the MRK strain. Anaplasma-specific DNA was detected in blood samples from 1 sheep in the Webster strain-inoculated group and 3 sheep in the MRK strain-inoculated group. Sheep seropositive for Anaplasma spp were detected in 5 of 8 flocks, and flocks in the Sierra Nevada foothills had higher within-flock seroprevalence (22%) than did flocks in the Oregon Coast Range (6.4%). Conclusions and Clinical Relevance-Infection with A phagocytophilum in mature sheep generally resulted in subclinical disease. Higher Anaplasma spp seroprevalence in sheep in the Sierra Nevada foothills corresponded to the geographic distribution of anaplasmosis reported for dogs, horses, and humans.

AB - Objective-To evaluate disease progression in sheep experimentally inoculated with Anaplasma phagocytophilum and determine the Anaplasma spp seroprevalence in sheep in free-ranging flocks in the Sierra Nevada foothills and Oregon Coast Range. Animals-10 mature ewes seronegative for Anaplasma spp and 251 sheep from 8 flocks. Procedures-10 ewes received 1 of 3 treatments: A phagocytophilum Webster strain (n =4), A phagocytophilum MRK strain (4), or human promyelocytic leukemia cells (control treatment [2]). Sheep were monitored for signs of clinical disease, and blood samples were obtained for serologic and PCR assay evaluation intermittently for 48 days. From a subsample of sheep from each of 8 free-ranging flocks, blood samples were obtained to determine Anaplasma spp seroprevalence. Results-Sheep inoculated with A phagocytophilum developed subclinical or mild disease, whereas sheep inoculated with the control treatment did not develop any signs of disease. Only 2 ewes seroconverted; both had received the MRK strain. Anaplasma-specific DNA was detected in blood samples from 1 sheep in the Webster strain-inoculated group and 3 sheep in the MRK strain-inoculated group. Sheep seropositive for Anaplasma spp were detected in 5 of 8 flocks, and flocks in the Sierra Nevada foothills had higher within-flock seroprevalence (22%) than did flocks in the Oregon Coast Range (6.4%). Conclusions and Clinical Relevance-Infection with A phagocytophilum in mature sheep generally resulted in subclinical disease. Higher Anaplasma spp seroprevalence in sheep in the Sierra Nevada foothills corresponded to the geographic distribution of anaplasmosis reported for dogs, horses, and humans.

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