Evaluating the long-term persistence of Bacillus spores on common surfaces

Kyle S. Enger, Jade Mitchell, Bharathi Murali, Dawn N. Birdsell, Paul S Keim, Patrick L. Gurian, David M Wagner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Bacillus spores resist inactivation, but the extent of their persistence on common surfaces is unclear. This work addresses knowledge gaps regarding biothreat agents in the environment to reduce uncertainty in risk assessment models. Studies were conducted to investigate the long-term inactivation of Bacillus anthracis and three commonly used surrogate organisms – B. cereus, B. atrophaeus and B. thuringiensis on three materials: laminate countertop, stainless steel and polystyrene Petri dishes. Viable spores were measured at 1, 30, 90, 196, 304 and 1038 days. Twelve different persistence models were fit to the data using maximum likelihood estimation and compared. The study found that (1) spore inactivation was not log-linear, as commonly modelled; (2) B. thuringiensis counts increased at 24 h on all materials, followed by a subsequent decline; (3) several experiments showed evidence of a ‘U’ shape, with spore counts apparently decreasing and then increasing between 1 and 304 days; (4) spores on polystyrene showed little inactivation; and (5) the maximum inactivation of 56% was observed for B. atrophaeus spores on steel at 196 days. Over the range of surfaces, time durations and conditions (humidity controlled vs. uncontrolled) examined, B. thuringiensis most closely matched the behaviour of B. anthracis.

Original languageEnglish (US)
Pages (from-to)1048-1059
Number of pages12
JournalMicrobial Biotechnology
Volume11
Issue number6
DOIs
StatePublished - Nov 1 2018

Fingerprint

Polystyrenes
Bacilli
Spores
Bacillus
Maximum likelihood estimation
Steel
Stainless Steel
Risk assessment
Laminates
Atmospheric humidity
Stainless steel
Microbial Colony Count
Bacillus anthracis
Humidity
Experiments
Uncertainty

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Evaluating the long-term persistence of Bacillus spores on common surfaces. / Enger, Kyle S.; Mitchell, Jade; Murali, Bharathi; Birdsell, Dawn N.; Keim, Paul S; Gurian, Patrick L.; Wagner, David M.

In: Microbial Biotechnology, Vol. 11, No. 6, 01.11.2018, p. 1048-1059.

Research output: Contribution to journalArticle

Enger, Kyle S. ; Mitchell, Jade ; Murali, Bharathi ; Birdsell, Dawn N. ; Keim, Paul S ; Gurian, Patrick L. ; Wagner, David M. / Evaluating the long-term persistence of Bacillus spores on common surfaces. In: Microbial Biotechnology. 2018 ; Vol. 11, No. 6. pp. 1048-1059.
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