Eccentric contraction

Unraveling mechanisms of force enhancement and energy conservation

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

During the past century, physiologists have made steady progress in elucidating the molecular mechanisms of muscle contraction. However, this progress has so far failed to definitively explain the high force and low energy cost of eccentric muscle contraction. Hypotheses that have been proposed to explain increased muscle force during active stretch include cross-bridge mechanisms, sarcomere and half-sarcomere length non-uniformity, and engagement of a structural element upon muscle activation. The available evidence suggests that force enhancement results from an interaction between an elastic element in muscle sarcomeres, which is engaged upon activation, and the cross-bridges, which interact with the elastic elements to regulate their length and stiffness. Similarities between titin-based residual force enhancement in vertebrate muscle and twitchin-based 'catch' in invertebrate muscle suggest evolutionary homology. The winding filament hypothesis suggests plausible molecular mechanisms for effects of both Ca2+ influx and cross-bridge cycling on titin in active muscle. This hypothesis proposes that the N2A region of titin binds to actin upon Ca2+ influx, and that the PEVK region of titin winds on the thin filaments during force development because the cross-bridges not only translate but also rotate the thin filaments. Simulations demonstrate that a muscle model based on the winding filament hypothesis can predict residual force enhancement on the descending limb of the length-tension curve in muscles during eccentric contraction. A kinematic model of titin winding based on sarcomere geometry makes testable predictions about titin isoforms in different muscles. Ongoing research is aimed at testing these predictions and elucidating the biochemistry of the underlying protein interactions.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalJournal of Experimental Biology
Volume219
Issue number2
DOIs
StatePublished - Jan 1 2016

Fingerprint

energy conservation
Connectin
contraction
muscle
Muscles
muscles
Sarcomeres
sarcomeres
muscle contraction
Muscle Contraction
calcium
prediction
energy costs
Invertebrates
physiologists
limbs (animal)
kinematics
Biomechanical Phenomena
Biochemistry
biochemistry

Keywords

  • Active stretch
  • Energy efficiency
  • Titin activation
  • Winding filament hypothesis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Cite this

Eccentric contraction : Unraveling mechanisms of force enhancement and energy conservation. / Nishikawa, Kiisa C.

In: Journal of Experimental Biology, Vol. 219, No. 2, 01.01.2016, p. 189-196.

Research output: Contribution to journalArticle

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