Dual modality live cell imaging with multiple-wavelength digital holography and epi-fluorescence

Christopher J Mann, Philip R. Bingham, Henry K. Lin, Vincent C. Paquit, Shaun S. Gleason

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We apply multiple-wavelength digital holography in combination with epi-fluorescence microscopy in order to generate quantitative phase and fluorescence information from cell samples. While digital holography provides high precision morphological information, the addition of fluorescence supplies the specificity needed to identify cellular constituents. By the application of multiple-wavelength digital holography it is possible to obtain the complete wavefront data deterministically and in real-time. We demonstrate this dual-mode imaging capability through the investigation of living cells. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
Journal3D Research
Volume2
Issue number1
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

Holography
Fluorescence
Imaging techniques
Wavelength
Fluorescence microscopy
Wavefronts
Cells

Keywords

  • and Interference microscopy
  • Computer holography
  • Holographic interferometry

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Software

Cite this

Dual modality live cell imaging with multiple-wavelength digital holography and epi-fluorescence. / Mann, Christopher J; Bingham, Philip R.; Lin, Henry K.; Paquit, Vincent C.; Gleason, Shaun S.

In: 3D Research, Vol. 2, No. 1, 2011, p. 1-6.

Research output: Contribution to journalArticle

Mann, Christopher J ; Bingham, Philip R. ; Lin, Henry K. ; Paquit, Vincent C. ; Gleason, Shaun S. / Dual modality live cell imaging with multiple-wavelength digital holography and epi-fluorescence. In: 3D Research. 2011 ; Vol. 2, No. 1. pp. 1-6.
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