DNA fingerprinting reveals low genetic diversity in Gunnison's prairie dog (Cynomys gunnisoni)

Steven E. Travis, C. N. Slobodchikoff, Paul S Keim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The use of molecular techniques for the assessment of familial relationships among social species of mammals has become relatively commonplace. However, some species represent poor candidates for such studies due to naturally low levels of genetic diversity, leading to unacceptably large standard errors associated with estimates of relatedness. Here, we report on a preliminary study of genetic diversity within two populations of a social species of ground squirrel, Gunnison's prairie dog (Cynomys gunnisoni) using DNA fingerprinting. We observed low levels of diversity in the form of large mean coefficients of genetic similarity among individuals occupying the same population. Overall similarity, determined from the combined data, yielded by three minisatellite probes, ranged from 55 to 61%. These values place Gunnison's prairie dog at the extreme upper end of the range of similarity values reported for outbred species of mammals (ca. 0.20-0.50). As a partial means of explaining these results, and as a means of comparing our results to those of similar studies using allozymes, we determined the level of differentiation between our two study colonies in the form of an F-statistic analog. A value of 0.11 (± 2.26 × 10-3) was obtained and is similar to values reported from allozyme studies (0.07-0.12). A significance test of this value yielded a positive result (D = 5.63, d.f. = 1, P < 0.025), demonstrating that gene flow between populations is limited, a factor that may help to maintain low levels of diversity.

Original languageEnglish (US)
Pages (from-to)725-732
Number of pages8
JournalJournal of Mammalogy
Volume78
Issue number3
StatePublished - Aug 1997

Fingerprint

Cynomys
Sciuridae
DNA fingerprinting
DNA Fingerprinting
prairie
allozymes
genetic variation
Isoenzymes
Mammals
allozyme
mammals
Population
Minisatellite Repeats
mammal
Gene Flow
minisatellite repeats
squirrels
gene flow
statistics
relatedness

Keywords

  • Cynomys gunnisoni
  • DNA fingerprinting
  • Genetic diversity
  • Population differentiation

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

DNA fingerprinting reveals low genetic diversity in Gunnison's prairie dog (Cynomys gunnisoni). / Travis, Steven E.; Slobodchikoff, C. N.; Keim, Paul S.

In: Journal of Mammalogy, Vol. 78, No. 3, 08.1997, p. 725-732.

Research output: Contribution to journalArticle

Travis, Steven E. ; Slobodchikoff, C. N. ; Keim, Paul S. / DNA fingerprinting reveals low genetic diversity in Gunnison's prairie dog (Cynomys gunnisoni). In: Journal of Mammalogy. 1997 ; Vol. 78, No. 3. pp. 725-732.
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