Diversity of 16s-23s rDNA internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors

Andrew P. Liguori, Stephanie D. Warrington, Jennifer L. Ginther, Talima R Pearson, Jolene Bowers, Mindy B. Glass, Mark Mayo, Vanaporn Wuthiekanun, David Engelthaler, Sharon J. Peacock, Bart J. Currie, David M Wagner, Paul S Keim, Apichai Tuanyok

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Length polymorphisms within the 16S-23S ribosomal DNA internal transcribed spacer (ITS) have been described as stable genetic markers for studying bacterial phylogenetics. In this study, we used these genetic markers to investigate phylogenetic relationships in Burkholderia pseudomallei and its near-relative species. B. pseudomallei is known as one of the most genetically recombined bacterial species. In silico analysis of multiple B. pseudomallei genomes revealed approximately four homologous rRNA operons and ITS length polymorphisms therein. We characterized ITS distribution using PCR and analyzed via a high-throughput capillary electrophoresis in 1,191 B. pseudomallei strains. Three major ITS types were identified, two of which were commonly found in most B. pseudomallei strains from the endemic areas, whereas the third one was significantly correlated with worldwide sporadic strains. Interestingly, mixtures of the two common ITS types were observed within the same strains, and at a greater incidence in Thailand than Australia suggesting that genetic recombination causes the ITS variation within species, with greater recombination frequency in Thailand. In addition, the B. mallei ITS type was common to B. pseudomallei, providing further support that B. mallei is a clone of B. pseudomallei. Other B. pseudomallei near-neighbors possessed unique and monomorphic ITS types. Our data shed light on evolutionary patterns of B. pseudomallei and its near relative species.

Original languageEnglish (US)
Article numbere29323
JournalPLoS One
Volume6
Issue number12
DOIs
StatePublished - Dec 14 2011

Fingerprint

Burkholderia pseudomallei
Ribosomal DNA
internal transcribed spacers
phylogeny
Polymorphism
Malleus
Capillary electrophoresis
Thailand
Genetic Markers
Genetic Recombination
Genes
Throughput
rRNA Operon
genetic polymorphism
genetic recombination
Capillary Electrophoresis
genetic markers
capillary electrophoresis
operon
Computer Simulation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Diversity of 16s-23s rDNA internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors. / Liguori, Andrew P.; Warrington, Stephanie D.; Ginther, Jennifer L.; Pearson, Talima R; Bowers, Jolene; Glass, Mindy B.; Mayo, Mark; Wuthiekanun, Vanaporn; Engelthaler, David; Peacock, Sharon J.; Currie, Bart J.; Wagner, David M; Keim, Paul S; Tuanyok, Apichai.

In: PLoS One, Vol. 6, No. 12, e29323, 14.12.2011.

Research output: Contribution to journalArticle

Liguori, AP, Warrington, SD, Ginther, JL, Pearson, TR, Bowers, J, Glass, MB, Mayo, M, Wuthiekanun, V, Engelthaler, D, Peacock, SJ, Currie, BJ, Wagner, DM, Keim, PS & Tuanyok, A 2011, 'Diversity of 16s-23s rDNA internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors', PLoS One, vol. 6, no. 12, e29323. https://doi.org/10.1371/journal.pone.0029323
Liguori, Andrew P. ; Warrington, Stephanie D. ; Ginther, Jennifer L. ; Pearson, Talima R ; Bowers, Jolene ; Glass, Mindy B. ; Mayo, Mark ; Wuthiekanun, Vanaporn ; Engelthaler, David ; Peacock, Sharon J. ; Currie, Bart J. ; Wagner, David M ; Keim, Paul S ; Tuanyok, Apichai. / Diversity of 16s-23s rDNA internal transcribed spacer (its) reveals phylogenetic relationships in burkholderia pseudomallei and its near-neighbors. In: PLoS One. 2011 ; Vol. 6, No. 12.
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