Diverse genotypes of Yersinia pestis caused plague in Madagascar in 2007

Julia M. Riehm, Michaela Projahn, Amy J. Vogler, Minoaerisoa Rajerison, Genevieve Andersen, Carina M. Hall, Thomas Zimmermann, Rahelinirina Soanandrasana, Voahangy Andrianaivoarimanana, Reinhard K. Straubinger, Roxanne Nottingham, Paul S Keim, David M Wagner, Holger C. Scholz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background Yersinia pestis is the causative agent of human plague and is endemic in various African, Asian and American countries. In Madagascar, the disease represents a significant public health problem with hundreds of human cases a year. Unfortunately, poor infrastructure makes outbreak investigations challenging. Methodology/Principal Findings DNA was extracted directly from 93 clinical samples from patients with a clinical diagnosis of plague in Madagascar in 2007. The extracted DNAs were then genotyped using three molecular genotyping methods, including, single nucleotide polymorphism (SNP) typing, multi-locus variable-number tandem repeat analysis (MLVA), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) analysis. These methods provided increasing resolution, respectively. The results of these analyses revealed that, in 2007, ten molecular groups, two newly described here and eight previously identified, were responsible for causing human plague in geographically distinct areas of Madagascar. Conclusions/Significance Plague in Madagascar is caused by numerous distinct types of Y. pestis. Genotyping method choice should be based upon the discriminatory power needed, expense, and available data for any desired comparisons. We conclude that genotyping should be a standard tool used in epidemiological investigations of plague outbreaks.

Original languageEnglish (US)
Article numbere0003844
JournalPLoS Neglected Tropical Diseases
Volume9
Issue number6
DOIs
StatePublished - Jun 12 2015

Fingerprint

Madagascar
Yersinia pestis
Plague
Genotype
Disease Outbreaks
Clustered Regularly Interspaced Short Palindromic Repeats
Minisatellite Repeats
Asian Americans
DNA
African Americans
Single Nucleotide Polymorphism
Public Health

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Riehm, J. M., Projahn, M., Vogler, A. J., Rajerison, M., Andersen, G., Hall, C. M., ... Scholz, H. C. (2015). Diverse genotypes of Yersinia pestis caused plague in Madagascar in 2007. PLoS Neglected Tropical Diseases, 9(6), [e0003844]. https://doi.org/10.1371/journal.pntd.0003844

Diverse genotypes of Yersinia pestis caused plague in Madagascar in 2007. / Riehm, Julia M.; Projahn, Michaela; Vogler, Amy J.; Rajerison, Minoaerisoa; Andersen, Genevieve; Hall, Carina M.; Zimmermann, Thomas; Soanandrasana, Rahelinirina; Andrianaivoarimanana, Voahangy; Straubinger, Reinhard K.; Nottingham, Roxanne; Keim, Paul S; Wagner, David M; Scholz, Holger C.

In: PLoS Neglected Tropical Diseases, Vol. 9, No. 6, e0003844, 12.06.2015.

Research output: Contribution to journalArticle

Riehm, JM, Projahn, M, Vogler, AJ, Rajerison, M, Andersen, G, Hall, CM, Zimmermann, T, Soanandrasana, R, Andrianaivoarimanana, V, Straubinger, RK, Nottingham, R, Keim, PS, Wagner, DM & Scholz, HC 2015, 'Diverse genotypes of Yersinia pestis caused plague in Madagascar in 2007', PLoS Neglected Tropical Diseases, vol. 9, no. 6, e0003844. https://doi.org/10.1371/journal.pntd.0003844
Riehm JM, Projahn M, Vogler AJ, Rajerison M, Andersen G, Hall CM et al. Diverse genotypes of Yersinia pestis caused plague in Madagascar in 2007. PLoS Neglected Tropical Diseases. 2015 Jun 12;9(6). e0003844. https://doi.org/10.1371/journal.pntd.0003844
Riehm, Julia M. ; Projahn, Michaela ; Vogler, Amy J. ; Rajerison, Minoaerisoa ; Andersen, Genevieve ; Hall, Carina M. ; Zimmermann, Thomas ; Soanandrasana, Rahelinirina ; Andrianaivoarimanana, Voahangy ; Straubinger, Reinhard K. ; Nottingham, Roxanne ; Keim, Paul S ; Wagner, David M ; Scholz, Holger C. / Diverse genotypes of Yersinia pestis caused plague in Madagascar in 2007. In: PLoS Neglected Tropical Diseases. 2015 ; Vol. 9, No. 6.
@article{6fcf90dd3aec4c07ac74c9dafd7dd557,
title = "Diverse genotypes of Yersinia pestis caused plague in Madagascar in 2007",
abstract = "Background Yersinia pestis is the causative agent of human plague and is endemic in various African, Asian and American countries. In Madagascar, the disease represents a significant public health problem with hundreds of human cases a year. Unfortunately, poor infrastructure makes outbreak investigations challenging. Methodology/Principal Findings DNA was extracted directly from 93 clinical samples from patients with a clinical diagnosis of plague in Madagascar in 2007. The extracted DNAs were then genotyped using three molecular genotyping methods, including, single nucleotide polymorphism (SNP) typing, multi-locus variable-number tandem repeat analysis (MLVA), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) analysis. These methods provided increasing resolution, respectively. The results of these analyses revealed that, in 2007, ten molecular groups, two newly described here and eight previously identified, were responsible for causing human plague in geographically distinct areas of Madagascar. Conclusions/Significance Plague in Madagascar is caused by numerous distinct types of Y. pestis. Genotyping method choice should be based upon the discriminatory power needed, expense, and available data for any desired comparisons. We conclude that genotyping should be a standard tool used in epidemiological investigations of plague outbreaks.",
author = "Riehm, {Julia M.} and Michaela Projahn and Vogler, {Amy J.} and Minoaerisoa Rajerison and Genevieve Andersen and Hall, {Carina M.} and Thomas Zimmermann and Rahelinirina Soanandrasana and Voahangy Andrianaivoarimanana and Straubinger, {Reinhard K.} and Roxanne Nottingham and Keim, {Paul S} and Wagner, {David M} and Scholz, {Holger C.}",
year = "2015",
month = "6",
day = "12",
doi = "10.1371/journal.pntd.0003844",
language = "English (US)",
volume = "9",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2727",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - Diverse genotypes of Yersinia pestis caused plague in Madagascar in 2007

AU - Riehm, Julia M.

AU - Projahn, Michaela

AU - Vogler, Amy J.

AU - Rajerison, Minoaerisoa

AU - Andersen, Genevieve

AU - Hall, Carina M.

AU - Zimmermann, Thomas

AU - Soanandrasana, Rahelinirina

AU - Andrianaivoarimanana, Voahangy

AU - Straubinger, Reinhard K.

AU - Nottingham, Roxanne

AU - Keim, Paul S

AU - Wagner, David M

AU - Scholz, Holger C.

PY - 2015/6/12

Y1 - 2015/6/12

N2 - Background Yersinia pestis is the causative agent of human plague and is endemic in various African, Asian and American countries. In Madagascar, the disease represents a significant public health problem with hundreds of human cases a year. Unfortunately, poor infrastructure makes outbreak investigations challenging. Methodology/Principal Findings DNA was extracted directly from 93 clinical samples from patients with a clinical diagnosis of plague in Madagascar in 2007. The extracted DNAs were then genotyped using three molecular genotyping methods, including, single nucleotide polymorphism (SNP) typing, multi-locus variable-number tandem repeat analysis (MLVA), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) analysis. These methods provided increasing resolution, respectively. The results of these analyses revealed that, in 2007, ten molecular groups, two newly described here and eight previously identified, were responsible for causing human plague in geographically distinct areas of Madagascar. Conclusions/Significance Plague in Madagascar is caused by numerous distinct types of Y. pestis. Genotyping method choice should be based upon the discriminatory power needed, expense, and available data for any desired comparisons. We conclude that genotyping should be a standard tool used in epidemiological investigations of plague outbreaks.

AB - Background Yersinia pestis is the causative agent of human plague and is endemic in various African, Asian and American countries. In Madagascar, the disease represents a significant public health problem with hundreds of human cases a year. Unfortunately, poor infrastructure makes outbreak investigations challenging. Methodology/Principal Findings DNA was extracted directly from 93 clinical samples from patients with a clinical diagnosis of plague in Madagascar in 2007. The extracted DNAs were then genotyped using three molecular genotyping methods, including, single nucleotide polymorphism (SNP) typing, multi-locus variable-number tandem repeat analysis (MLVA), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) analysis. These methods provided increasing resolution, respectively. The results of these analyses revealed that, in 2007, ten molecular groups, two newly described here and eight previously identified, were responsible for causing human plague in geographically distinct areas of Madagascar. Conclusions/Significance Plague in Madagascar is caused by numerous distinct types of Y. pestis. Genotyping method choice should be based upon the discriminatory power needed, expense, and available data for any desired comparisons. We conclude that genotyping should be a standard tool used in epidemiological investigations of plague outbreaks.

UR - http://www.scopus.com/inward/record.url?scp=84934784203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934784203&partnerID=8YFLogxK

U2 - 10.1371/journal.pntd.0003844

DO - 10.1371/journal.pntd.0003844

M3 - Article

C2 - 26069964

AN - SCOPUS:84934784203

VL - 9

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2727

IS - 6

M1 - e0003844

ER -