Distribution of restriction site polymorphism within the chloroplast genome of the genus Glycine, subgenus Soja

P. S. Close, R. C. Shoemaker, Paul S Keim

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Restriction fragment length polymorphisms (RFLPs) have been used to detect intragenic sequence diversity in Glycine subgenus soja chloroplast DNA. The distribution of these RFLPs allow Glycine max and G. soja accessions to be grouped according to cytoplasmic genetic relatedness. DNA clones from mung bean chloroplast DNA were used to locate the RFLPs to specific regions of the chloroplast genome. In the course of the experiments, several previously unobserved RFLPs were also identified. At least six molecular changes were detected, including both restriction site loss or gain and insertion/deletion events. Three of the fragment polymorphisms detected are due to changes in the juncture region between one inverted repeat region and the large single-copy region. Probes detecting polymorphisms in three representative soybean genotypes were used to screen additional cultivars and Plant Introductions. The distribution of RFLP patterns in these accessions were consistent with the patterns of previously described cytoplasmic groupings, with the exception of one accession, which formed a new plastome group.

Original languageEnglish (US)
Pages (from-to)768-776
Number of pages9
JournalTheoretical And Applied Genetics
Volume77
Issue number6
DOIs
StatePublished - Jun 1989

Fingerprint

Chloroplast Genome
Glycine (Fabaceae)
Restriction Fragment Length Polymorphisms
Glycine
restriction fragment length polymorphism
genetic polymorphism
Chloroplast DNA
chloroplast DNA
Soybeans
Glycine soja
introduced plants
mung beans
genetic relationships
Glycine max
Clone Cells
Genotype
chloroplast genome
soybeans
clones
genotype

Keywords

  • Chloroplast DNA
  • RFLP
  • Soybean

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Distribution of restriction site polymorphism within the chloroplast genome of the genus Glycine, subgenus Soja. / Close, P. S.; Shoemaker, R. C.; Keim, Paul S.

In: Theoretical And Applied Genetics, Vol. 77, No. 6, 06.1989, p. 768-776.

Research output: Contribution to journalArticle

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