Development of polymorphic tetranucleotide microsatellites for pinyon jays (Gymnorhinus cyanocephalus)

Joseph D. Busch, Russell Benford, Talima R Pearson, Eldon Palmer, Russell P. Balda, Paul S Keim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The pinyon jay (Gymnorhinus cyanocephalus) is a primary seed disperser of pinyon pines (Pinus edulis and P. monophylla). Both the pinyon jay and the pinyon pines are experiencing significant decline. While the pinyon jay is a species of management value and conservation concern, little is known about its fecundity, among-flock dispersal, and population differentiation. We initiated genetic studies in pinyon jays using a hybridization enrichment technique to isolate seven polymorphic microsatellite repeats (AAAG and GATA) from the pinyon jay genome. A locus from the Mexican jay (Aphelocoma ultramarina) that amplifies robustly in pinyon jays is also reported. These eight loci revealed moderate to high diversity in an Arizona population of pinyon jays (4-36 alleles and H O 0.42-0.90). As in other species, tetranucleotide repeats produced easily resolved amplification products.

Original languageEnglish (US)
Pages (from-to)689-691
Number of pages3
JournalConservation Genetics
Volume10
Issue number3
DOIs
StatePublished - Jun 2009

Fingerprint

Pinus monophylla
Pinus edulis
Pinus
Microsatellite Repeats
microsatellite repeats
loci
Population
Fertility
fecundity
amplification
Seeds
allele
flocks
hybridization
genome
Alleles
Genome
alleles
seed
seeds

Keywords

  • Conservation
  • Gymnorhinus cyanocephalus
  • Hybridization enrichment
  • Pinyon pine
  • Tetranucleotide

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Development of polymorphic tetranucleotide microsatellites for pinyon jays (Gymnorhinus cyanocephalus). / Busch, Joseph D.; Benford, Russell; Pearson, Talima R; Palmer, Eldon; Balda, Russell P.; Keim, Paul S.

In: Conservation Genetics, Vol. 10, No. 3, 06.2009, p. 689-691.

Research output: Contribution to journalArticle

Busch, Joseph D. ; Benford, Russell ; Pearson, Talima R ; Palmer, Eldon ; Balda, Russell P. ; Keim, Paul S. / Development of polymorphic tetranucleotide microsatellites for pinyon jays (Gymnorhinus cyanocephalus). In: Conservation Genetics. 2009 ; Vol. 10, No. 3. pp. 689-691.
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