Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii

Kelly W. Sheff, Emily R. York, Elizabeth M. Driebe, Bridget M. Barker, Steven D. Rounsley, Victor G. Waddell, Stephen M Beckstrom-Sternberg, James S. Beckstrom-Sternberg, Paul S Keim, David M. Engelthaler

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

Original languageEnglish (US)
Pages (from-to)466-469
Number of pages4
JournalMedical Mycology
Volume48
Issue number3
DOIs
StatePublished - 2010

Fingerprint

Coccidioides immitis
Coccidioides
Coccidioidomycosis
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
coccidioidomycosis
Costs and Cost Analysis
disease surveillance
assays
natural history
infection
Infection
Coccidioides posadasii

Keywords

  • Coccidioides
  • Coccidioidomycosis
  • Epidemiology

ASJC Scopus subject areas

  • veterinary(all)
  • Infectious Diseases

Cite this

Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii. / Sheff, Kelly W.; York, Emily R.; Driebe, Elizabeth M.; Barker, Bridget M.; Rounsley, Steven D.; Waddell, Victor G.; Beckstrom-Sternberg, Stephen M; Beckstrom-Sternberg, James S.; Keim, Paul S; Engelthaler, David M.

In: Medical Mycology, Vol. 48, No. 3, 2010, p. 466-469.

Research output: Contribution to journalArticle

Sheff, Kelly W. ; York, Emily R. ; Driebe, Elizabeth M. ; Barker, Bridget M. ; Rounsley, Steven D. ; Waddell, Victor G. ; Beckstrom-Sternberg, Stephen M ; Beckstrom-Sternberg, James S. ; Keim, Paul S ; Engelthaler, David M. / Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii. In: Medical Mycology. 2010 ; Vol. 48, No. 3. pp. 466-469.
@article{0ab3acd8e41040ff997fe117b809bd54,
title = "Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii",
abstract = "Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.",
keywords = "Coccidioides, Coccidioidomycosis, Epidemiology",
author = "Sheff, {Kelly W.} and York, {Emily R.} and Driebe, {Elizabeth M.} and Barker, {Bridget M.} and Rounsley, {Steven D.} and Waddell, {Victor G.} and Beckstrom-Sternberg, {Stephen M} and Beckstrom-Sternberg, {James S.} and Keim, {Paul S} and Engelthaler, {David M.}",
year = "2010",
doi = "10.3109/13693780903218990",
language = "English (US)",
volume = "48",
pages = "466--469",
journal = "Medical Mycology",
issn = "1369-3786",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii

AU - Sheff, Kelly W.

AU - York, Emily R.

AU - Driebe, Elizabeth M.

AU - Barker, Bridget M.

AU - Rounsley, Steven D.

AU - Waddell, Victor G.

AU - Beckstrom-Sternberg, Stephen M

AU - Beckstrom-Sternberg, James S.

AU - Keim, Paul S

AU - Engelthaler, David M.

PY - 2010

Y1 - 2010

N2 - Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

AB - Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

KW - Coccidioides

KW - Coccidioidomycosis

KW - Epidemiology

UR - http://www.scopus.com/inward/record.url?scp=77950556060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950556060&partnerID=8YFLogxK

U2 - 10.3109/13693780903218990

DO - 10.3109/13693780903218990

M3 - Article

VL - 48

SP - 466

EP - 469

JO - Medical Mycology

JF - Medical Mycology

SN - 1369-3786

IS - 3

ER -