Development of a rapid, cost-effective TaqMan real-time PCR assay for identification and differentiation of coccidioides immitis and coccidioides posadasii

Kelly W. Sheff, Emily R. York, Elizabeth M. Driebe, Bridget M. Barker, Steven D. Rounsley, Victor G. Waddell, Stephen M. Beckstrom-Sternberg, James S. Beckstrom-Sternberg, Paul S. Keim, David M. Engelthaler

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

Original languageEnglish (US)
Pages (from-to)466-469
Number of pages4
JournalMedical Mycology
Volume48
Issue number3
DOIs
StatePublished - Apr 14 2010

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Keywords

  • Coccidioides
  • Coccidioidomycosis
  • Epidemiology

ASJC Scopus subject areas

  • Infectious Diseases

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