Detection of fatty acids from intact microorganisms by molecular beam static secondary ion mass spectrometry

Jani C Ingram, William F. Bauer, R. Michael Lehman, Sean P. O'Connell, Andrew D. Shaw

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We report the use of a surface analysis approach, static secondary ion mass spectrometry (SIMS) equipped with a molecular (ReO4-) ion primary beam, to analyze the surface of intact microbial cells. SIMS spectra of 28 microorganisms were compared to fatty acid profiles determined by gas chromatographic analysis of transesterfied fatty acids extracted from the same organisms. The results indicate that surface bombardment using the molecular primary beam cleaved the ester linkage characteristic of bacteria at the glycerophosphate backbone of the phospholipid components of the cell membrane. This cleavage enables direct detection of the fatty acid conjugate base of intact microorganisms by static SIMS. The limit of detection for this approach is approximately 107 bacterial cells/cm2. Multivariate statistical methods were applied in a graded approach to the SIMS microbial data. The results showed that the full data set could initially be statistically grouped based upon major differences in biochemical composition of the cell wall. The gram-positive bacteria were further statistically analyzed, followed by final analysis of a specific bacterial genus that was successfully grouped by species. Additionally, the use of SIMS to detect microbes on mineral surfaces is demonstrated by an analysis of Shewanella oneidensis on crushed hematite. The results of this study provide evidence for the potential of static SIMS to rapidly detect bacterial species based on ion fragments originating from cell membrane lipids directly from sample surfaces.

Original languageEnglish (US)
Pages (from-to)295-307
Number of pages13
JournalJournal of Microbiological Methods
Volume53
Issue number3
DOIs
StatePublished - Jun 1 2003

Fingerprint

Secondary Ion Mass Spectrometry
Fatty Acids
Shewanella
Ions
Glycerophosphates
Gram-Positive Bacteria
Membrane Lipids
Gas Chromatography
Cell Wall
Minerals
Limit of Detection
Phospholipids
Esters
Cell Membrane
Bacteria

Keywords

  • Fatty acids
  • Microorganisms
  • Static secondary ion mass spectrometry

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Detection of fatty acids from intact microorganisms by molecular beam static secondary ion mass spectrometry. / Ingram, Jani C; Bauer, William F.; Lehman, R. Michael; O'Connell, Sean P.; Shaw, Andrew D.

In: Journal of Microbiological Methods, Vol. 53, No. 3, 01.06.2003, p. 295-307.

Research output: Contribution to journalArticle

Ingram, Jani C ; Bauer, William F. ; Lehman, R. Michael ; O'Connell, Sean P. ; Shaw, Andrew D. / Detection of fatty acids from intact microorganisms by molecular beam static secondary ion mass spectrometry. In: Journal of Microbiological Methods. 2003 ; Vol. 53, No. 3. pp. 295-307.
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