Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species

Joshua K. Stone, Mark Mayo, Stephanie A. Grasso, Jennifer L. Ginther, Stephanie D. Warrington, Christopher J. Allender, Adina Doyle, Shalamar Georgia, Mirjam Kaestli, Stacey M. Broomall, Mark A. Karavis, Joseph M. Insalaco, Kyle S. Hubbard, Lauren A. McNew, Henry S. Gibbons, Bart J. Currie, Paul S Keim, Apichai Tuanyok

Research output: Contribution to journalArticle

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Abstract

Background: Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes in B. pseudomallei. Complicating this strategy are the four different B. pseudomallei LPS O-antigen types: A, B, B2, and rough. Sero-crossreactivity is common among O-antigens of Burkholderia species. Here, we identified the presence of multiple B. pseudomallei O-antigen types and sero-crossreactivity in its near-neighbor species. Results: PCR screening of O-antigen biosynthesis genes, phenotypic characterization using SDS-PAGE, and immunoblot analysis showed that majority of B. mallei and B. thailandensis strains contained the typical O-antigen type A. In contrast, most of B. ubonensis and B. thailandensis-like strains expressed the atypical O-antigen types B and B2, respectively. Most B. oklahomensis strains expressed a distinct and non-seroreactive O-antigen type, except strain E0147 which expressed O-antigen type A. O-antigen type B2 was also detected in B. thailandensis 82172, B. ubonensis MSMB108, and Burkholderia sp. MSMB175. Interestingly, B. thailandensis-like MSMB43 contained a novel serotype B positive O-antigen. Conclusions: This study expands the number of species which express B. pseudomallei O-antigen types. Further work is required to elucidate the full structures and how closely these are to the B. pseudomallei O-antigens, which will ultimately determine the efficacy of the near-neighbor B serotypes for vaccine development.

Original languageEnglish (US)
Article number250
JournalBMC Microbiology
Volume12
DOIs
StatePublished - 2012

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Burkholderia pseudomallei
O Antigens
Burkholderia
Vaccines
Lipopolysaccharides
Serogroup
Malleus
Melioidosis
Centers for Disease Control and Prevention (U.S.)
Epitopes
Polyacrylamide Gel Electrophoresis
Immunization

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Stone, J. K., Mayo, M., Grasso, S. A., Ginther, J. L., Warrington, S. D., Allender, C. J., ... Tuanyok, A. (2012). Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species. BMC Microbiology, 12, [250]. https://doi.org/10.1186/1471-2180-12-250

Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species. / Stone, Joshua K.; Mayo, Mark; Grasso, Stephanie A.; Ginther, Jennifer L.; Warrington, Stephanie D.; Allender, Christopher J.; Doyle, Adina; Georgia, Shalamar; Kaestli, Mirjam; Broomall, Stacey M.; Karavis, Mark A.; Insalaco, Joseph M.; Hubbard, Kyle S.; McNew, Lauren A.; Gibbons, Henry S.; Currie, Bart J.; Keim, Paul S; Tuanyok, Apichai.

In: BMC Microbiology, Vol. 12, 250, 2012.

Research output: Contribution to journalArticle

Stone, JK, Mayo, M, Grasso, SA, Ginther, JL, Warrington, SD, Allender, CJ, Doyle, A, Georgia, S, Kaestli, M, Broomall, SM, Karavis, MA, Insalaco, JM, Hubbard, KS, McNew, LA, Gibbons, HS, Currie, BJ, Keim, PS & Tuanyok, A 2012, 'Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species', BMC Microbiology, vol. 12, 250. https://doi.org/10.1186/1471-2180-12-250
Stone JK, Mayo M, Grasso SA, Ginther JL, Warrington SD, Allender CJ et al. Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species. BMC Microbiology. 2012;12. 250. https://doi.org/10.1186/1471-2180-12-250
Stone, Joshua K. ; Mayo, Mark ; Grasso, Stephanie A. ; Ginther, Jennifer L. ; Warrington, Stephanie D. ; Allender, Christopher J. ; Doyle, Adina ; Georgia, Shalamar ; Kaestli, Mirjam ; Broomall, Stacey M. ; Karavis, Mark A. ; Insalaco, Joseph M. ; Hubbard, Kyle S. ; McNew, Lauren A. ; Gibbons, Henry S. ; Currie, Bart J. ; Keim, Paul S ; Tuanyok, Apichai. / Detection of Burkholderia pseudomallei O-antigen serotypes in near-neighbor species. In: BMC Microbiology. 2012 ; Vol. 12.
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abstract = "Background: Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes in B. pseudomallei. Complicating this strategy are the four different B. pseudomallei LPS O-antigen types: A, B, B2, and rough. Sero-crossreactivity is common among O-antigens of Burkholderia species. Here, we identified the presence of multiple B. pseudomallei O-antigen types and sero-crossreactivity in its near-neighbor species. Results: PCR screening of O-antigen biosynthesis genes, phenotypic characterization using SDS-PAGE, and immunoblot analysis showed that majority of B. mallei and B. thailandensis strains contained the typical O-antigen type A. In contrast, most of B. ubonensis and B. thailandensis-like strains expressed the atypical O-antigen types B and B2, respectively. Most B. oklahomensis strains expressed a distinct and non-seroreactive O-antigen type, except strain E0147 which expressed O-antigen type A. O-antigen type B2 was also detected in B. thailandensis 82172, B. ubonensis MSMB108, and Burkholderia sp. MSMB175. Interestingly, B. thailandensis-like MSMB43 contained a novel serotype B positive O-antigen. Conclusions: This study expands the number of species which express B. pseudomallei O-antigen types. Further work is required to elucidate the full structures and how closely these are to the B. pseudomallei O-antigens, which will ultimately determine the efficacy of the near-neighbor B serotypes for vaccine development.",
author = "Stone, {Joshua K.} and Mark Mayo and Grasso, {Stephanie A.} and Ginther, {Jennifer L.} and Warrington, {Stephanie D.} and Allender, {Christopher J.} and Adina Doyle and Shalamar Georgia and Mirjam Kaestli and Broomall, {Stacey M.} and Karavis, {Mark A.} and Insalaco, {Joseph M.} and Hubbard, {Kyle S.} and McNew, {Lauren A.} and Gibbons, {Henry S.} and Currie, {Bart J.} and Keim, {Paul S} and Apichai Tuanyok",
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AU - Stone, Joshua K.

AU - Mayo, Mark

AU - Grasso, Stephanie A.

AU - Ginther, Jennifer L.

AU - Warrington, Stephanie D.

AU - Allender, Christopher J.

AU - Doyle, Adina

AU - Georgia, Shalamar

AU - Kaestli, Mirjam

AU - Broomall, Stacey M.

AU - Karavis, Mark A.

AU - Insalaco, Joseph M.

AU - Hubbard, Kyle S.

AU - McNew, Lauren A.

AU - Gibbons, Henry S.

AU - Currie, Bart J.

AU - Keim, Paul S

AU - Tuanyok, Apichai

PY - 2012

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N2 - Background: Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select agent with no available effective vaccine. Previous immunizations in mice have utilized the lipopolysaccharide (LPS) as a potential vaccine target because it is known as one of the most important antigenic epitopes in B. pseudomallei. Complicating this strategy are the four different B. pseudomallei LPS O-antigen types: A, B, B2, and rough. Sero-crossreactivity is common among O-antigens of Burkholderia species. Here, we identified the presence of multiple B. pseudomallei O-antigen types and sero-crossreactivity in its near-neighbor species. Results: PCR screening of O-antigen biosynthesis genes, phenotypic characterization using SDS-PAGE, and immunoblot analysis showed that majority of B. mallei and B. thailandensis strains contained the typical O-antigen type A. In contrast, most of B. ubonensis and B. thailandensis-like strains expressed the atypical O-antigen types B and B2, respectively. Most B. oklahomensis strains expressed a distinct and non-seroreactive O-antigen type, except strain E0147 which expressed O-antigen type A. O-antigen type B2 was also detected in B. thailandensis 82172, B. ubonensis MSMB108, and Burkholderia sp. MSMB175. Interestingly, B. thailandensis-like MSMB43 contained a novel serotype B positive O-antigen. Conclusions: This study expands the number of species which express B. pseudomallei O-antigen types. Further work is required to elucidate the full structures and how closely these are to the B. pseudomallei O-antigens, which will ultimately determine the efficacy of the near-neighbor B serotypes for vaccine development.

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