Detection by in-situ fluorescence of short, single-copy sequences of chromosomal DNA

Tong Zhu, Liang Shi, Paul S Keim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Short single-copy probes have been widely used in plant molecular biology. However, they have rarely been effective in plant research using in situ hybridization techniques, possibly due to limitations imposed by the cell wall. We recently developed two fluorescence in situ hybridization protocols for the single-copy sequence detection in soybean. By enzymatically removing the cell wall, single-copy sequences as short as 1 kb were detected by probes using standard fluorescence in situ hybridization or PCR-primed in situ hybridization (PCR-PRINS). Such technology is useful for genome analysis, in plant molecular, cellular, and biotechnological research.

Original languageEnglish (US)
Pages (from-to)270-277
Number of pages8
JournalPlant Molecular Biology Reporter
Volume13
Issue number3
DOIs
StatePublished - Sep 1995

Fingerprint

fluorescence in situ hybridization
in situ hybridization
Fluorescence
Cells
cell walls
fluorescence
Fluorescence In Situ Hybridization
Cell Wall
Molecular biology
In Situ Hybridization
DNA
plant biology
molecular biology
Genes
soybeans
Soybeans
Research
genome
Molecular Biology
Genome

Keywords

  • Fluorescence in situ hybridization
  • PCR-primed in situ hybridization (PCR-PRINS)
  • RFLP loci
  • soybean (Glycine max)

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science
  • Molecular Biology

Cite this

Detection by in-situ fluorescence of short, single-copy sequences of chromosomal DNA. / Zhu, Tong; Shi, Liang; Keim, Paul S.

In: Plant Molecular Biology Reporter, Vol. 13, No. 3, 09.1995, p. 270-277.

Research output: Contribution to journalArticle

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