Detection by in-situ fluorescence of short, single-copy sequences of chromosomal DNA

Tong Zhu, Liang Shi, Paul Keim

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Short single-copy probes have been widely used in plant molecular biology. However, they have rarely been effective in plant research using in situ hybridization techniques, possibly due to limitations imposed by the cell wall. We recently developed two fluorescence in situ hybridization protocols for the single-copy sequence detection in soybean. By enzymatically removing the cell wall, single-copy sequences as short as 1 kb were detected by probes using standard fluorescence in situ hybridization or PCR-primed in situ hybridization (PCR-PRINS). Such technology is useful for genome analysis, in plant molecular, cellular, and biotechnological research.

Original languageEnglish (US)
Pages (from-to)270-277
Number of pages8
JournalPlant Molecular Biology Reporter
Volume13
Issue number3
DOIs
StatePublished - Sep 1 1995

Keywords

  • Fluorescence in situ hybridization
  • PCR-primed in situ hybridization (PCR-PRINS)
  • RFLP loci
  • soybean (Glycine max)

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

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