Defining seasonal marine microbial community dynamics

Jack A. Gilbert, Joshua A. Steele, James G Caporaso, Lars Steinbrück, Jens Reeder, Ben Temperton, Susan Huse, Alice C. McHardy, Rob Knight, Ian Joint, Paul Somerfield, Jed A. Fuhrman, Dawn Field

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464 Scopus citations

Abstract

Here we describe, the longest microbial time-series analyzed to date using high-resolution 16S rRNA tag pyrosequencing of samples taken monthly over 6 years at a temperate marine coastal site off Plymouth, UK. Data treatment effected the estimation of community richness over a 6-year period, whereby 8794 operational taxonomic units (OTUs) were identified using single-linkage preclustering and 21 130 OTUs were identified by denoising the data. The Alphaproteobacteria were the most abundant Class, and the most frequently recorded OTUs were members of the Rickettsiales (SAR 11) and Rhodobacteriales. This near-surface ocean bacterial community showed strong repeatable seasonal patterns, which were defined by winter peaks in diversity across all years. Environmental variables explained far more variation in seasonally predictable bacteria than did data on protists or metazoan biomass. Change in day length alone explains >65% of the variance in community diversity. The results suggested that seasonal changes in environmental variables are more important than trophic interactions. Interestingly, microbial association network analysis showed that correlations in abundance were stronger within bacterial taxa rather than between bacteria and eukaryotes, or between bacteria and environmental variables.

Original languageEnglish (US)
Pages (from-to)298-308
Number of pages11
JournalISME Journal
Volume6
Issue number2
DOIs
StatePublished - Feb 2012
Externally publishedYes

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Keywords

  • 16S rRNA
  • bacteria
  • community
  • diversity
  • microbial
  • model

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Cite this

Gilbert, J. A., Steele, J. A., Caporaso, J. G., Steinbrück, L., Reeder, J., Temperton, B., Huse, S., McHardy, A. C., Knight, R., Joint, I., Somerfield, P., Fuhrman, J. A., & Field, D. (2012). Defining seasonal marine microbial community dynamics. ISME Journal, 6(2), 298-308. https://doi.org/10.1038/ismej.2011.107