Computationally optimizing the compliance of multilayered biomimetic tissue engineered vascular grafts

Ehab A. Tamimi, Diana Catalina Ardila, Burt D. Ensley, Robert S Kellar, Jonathan P. Vande Geest

Research output: Contribution to journalArticle

Abstract

Coronary artery bypass grafts used to treat coronary artery disease (CAD) often fail due to compliance mismatch. In this study, we have developed an experimental/computational approach to fabricate an acellular biomimetic hybrid tissue engineered vascular graft (TEVG) composed of alternating layers of electrospun porcine gelatin/polycaprolactone (PCL) and human tropoelastin/PCL blends with the goal of compliance-matching to rat abdominal aorta, while maintaining specific geometrical constraints. Polymeric blends at three different gelatin:PCL (G:PCL) and tropoelastin:PCL (T:PCL) ratios (80:20, 50:50, and 20:80) were mechanically characterized. The stress-strain data were used to develop predictive models, which were used as part of an optimization scheme that was implemented to determine the ratios of G:PCL and T:PCL and the thickness of the individual layers within a TEVG that would compliance match a target compliance value. The hypocompliant, isocompliant, and hypercompliant grafts had target compliance values of 0.000256, 0.000568, and 0.000880mmHg-1, respectively. Experimental validation of the optimization demonstrated that the hypercompliant and isocompliant grafts were not statistically significant from their respective target compliance values (p-value=0.37 and 0.89, respectively). The experimental compliance values of the hypocompliant graft were statistically significant than their target compliance value (p-value=0.047). We have successfully demonstrated a design optimization scheme that can be used to fabricate multilayered and biomimetic vascular grafts with targeted geometry and compliance.

Original languageEnglish (US)
Article number061003
JournalJournal of Biomechanical Engineering
Volume141
Issue number6
DOIs
StatePublished - Jun 1 2019

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Blood Vessel Prosthesis
Biomimetics
Grafts
Compliance
Tissue
Polycaprolactone
Tropoelastin
Transplants
Gelatin
Abdominal Aorta
Coronary Artery Bypass
Blood Vessels
Rats
Coronary Artery Disease
Swine

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

Cite this

Computationally optimizing the compliance of multilayered biomimetic tissue engineered vascular grafts. / Tamimi, Ehab A.; Ardila, Diana Catalina; Ensley, Burt D.; Kellar, Robert S; Vande Geest, Jonathan P.

In: Journal of Biomechanical Engineering, Vol. 141, No. 6, 061003, 01.06.2019.

Research output: Contribution to journalArticle

Tamimi, Ehab A. ; Ardila, Diana Catalina ; Ensley, Burt D. ; Kellar, Robert S ; Vande Geest, Jonathan P. / Computationally optimizing the compliance of multilayered biomimetic tissue engineered vascular grafts. In: Journal of Biomechanical Engineering. 2019 ; Vol. 141, No. 6.
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