Comparison of two particulate hexavalent chromium compounds

Barium chromate is more genotoxic than lead chromate in human lung cells

Sandra S. Wise, Julie H C Schuler, Amie L. Holmes, Spiros P. Katsifis, Michael E Ketterer, Wendy J. Hartsock, Tonqzhanq Zheng, John Pierce Wise

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Particulate hexavalent chromium [Cr(VI)] compounds are well-established human lung carcinogens. However, their carcinogenic mechanisms are poorly understood as most investigators have used soluble Cr(VI) compounds. Recent work from our laboratory has found that barium chromate (BC) is also cytotoxic and clastogenic. To understand how BC relates to existing data on other particulate Cr(VI) compounds, we compared its cytotoxicity and clastogenicity with lead chromate (LC), which has been used as a prototypical particulate Cr(VI) compound, in WTHBF-6 cells, a near-normal human lung cell line. We found that BC is a more potent cytotoxicant, inducing 67%, 12%, 3%, and 0% relative survival at concentrations of 0.1, 0.5, 1, and 5 μg/cm2, respectively, while LC induced 90%, 71%, 43%, and 15% survival at these same concentrations. We found that BC was also more clastogenic, damaging 22% and 49% of metaphase cells at 0.1 and 0.5 μg/ cm2, and causing complete cell cycle arrest at 1 and 5 μg/cm2. By contrast, 0.1, 0.5, and 1.0 μg/cm2 LC damaged 10%, 27%, and 37% of metaphase cells, respectively, and complete cell cycle arrest was not observed until a concentration of 5 μg/cm2 was reached. We found that BC and LC both partially dissolved in complete medium in the presence of cells, producing similar extracellular concentrations. Both compounds were also comparable with respect to particle uptake and the amount of intracellular Cr ions. Considering previous reports showing that lead ions were inactive and that sodium chromate and LC have similar clastogenic potencies, these data suggest that BC genotoxicity may not be solely mediated by Cr ions, but also involve some clastogenic activity of barium ions.

Original languageEnglish (US)
Pages (from-to)156-162
Number of pages7
JournalEnvironmental and Molecular Mutagenesis
Volume44
Issue number2
DOIs
StatePublished - 2004

Fingerprint

Chromium Compounds
chromate
barium
chromium
Lung
Ions
Cells
Metaphase
Cell Cycle Checkpoints
Chromium compounds
Survival
ion
Barium
Cytotoxicity
Carcinogens
lead chromate
barium chromate
chromium hexavalent ion
comparison
Research Personnel

Keywords

  • Bronchial
  • Cell culture
  • Chromate, barium chromate
  • Chromium
  • Chromosome aberrations
  • Cytotoxicity
  • Genotoxicity

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Genetics
  • Genetics(clinical)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Comparison of two particulate hexavalent chromium compounds : Barium chromate is more genotoxic than lead chromate in human lung cells. / Wise, Sandra S.; Schuler, Julie H C; Holmes, Amie L.; Katsifis, Spiros P.; Ketterer, Michael E; Hartsock, Wendy J.; Zheng, Tonqzhanq; Wise, John Pierce.

In: Environmental and Molecular Mutagenesis, Vol. 44, No. 2, 2004, p. 156-162.

Research output: Contribution to journalArticle

Wise, Sandra S. ; Schuler, Julie H C ; Holmes, Amie L. ; Katsifis, Spiros P. ; Ketterer, Michael E ; Hartsock, Wendy J. ; Zheng, Tonqzhanq ; Wise, John Pierce. / Comparison of two particulate hexavalent chromium compounds : Barium chromate is more genotoxic than lead chromate in human lung cells. In: Environmental and Molecular Mutagenesis. 2004 ; Vol. 44, No. 2. pp. 156-162.
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