Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

Mirjam Kaestli, Leisha J. Richardson, Rebecca E. Colman, Apichai Tuanyok, Erin P. Price, Jolene R. Bowers, Mark Mayo, Erin Kelley, Meagan L. Seymour, Derek S. Sarovich, Talima Pearson, David M. Engelthaler, David M. Wagner, Paul S. Keim, James M. Schupp, Bart J. Currie

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28 Scopus citations

Abstract

Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

Original languageEnglish (US)
Pages (from-to)2059-2062
Number of pages4
JournalJournal of Clinical Microbiology
Volume50
Issue number6
DOIs
StatePublished - Jun 1 2012

ASJC Scopus subject areas

  • Microbiology (medical)

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    Kaestli, M., Richardson, L. J., Colman, R. E., Tuanyok, A., Price, E. P., Bowers, J. R., Mayo, M., Kelley, E., Seymour, M. L., Sarovich, D. S., Pearson, T., Engelthaler, D. M., Wagner, D. M., Keim, P. S., Schupp, J. M., & Currie, B. J. (2012). Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens. Journal of Clinical Microbiology, 50(6), 2059-2062. https://doi.org/10.1128/JCM.06737-11