Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

Mirjam Kaestli, Leisha J. Richardson, Rebecca E. Colman, Apichai Tuanyok, Erin P. Price, Jolene R. Bowers, Mark Mayo, Erin Kelley, Meagan L. Seymour, Derek S. Sarovich, Talima R Pearson, David M. Engelthaler, David M Wagner, Paul S Keim, James M. Schupp, Bart J. Currie

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

Original languageEnglish (US)
Pages (from-to)2059-2062
Number of pages4
JournalJournal of Clinical Microbiology
Volume50
Issue number6
DOIs
StatePublished - Jun 2012

Fingerprint

Melioidosis
Burkholderia pseudomallei
Emerging Communicable Diseases
Burkholderia
Yersinia
Polymerase Chain Reaction
Flagella
Chemotaxis
Limit of Detection
Soil
Bacteria

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Kaestli, M., Richardson, L. J., Colman, R. E., Tuanyok, A., Price, E. P., Bowers, J. R., ... Currie, B. J. (2012). Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens. Journal of Clinical Microbiology, 50(6), 2059-2062. https://doi.org/10.1128/JCM.06737-11

Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens. / Kaestli, Mirjam; Richardson, Leisha J.; Colman, Rebecca E.; Tuanyok, Apichai; Price, Erin P.; Bowers, Jolene R.; Mayo, Mark; Kelley, Erin; Seymour, Meagan L.; Sarovich, Derek S.; Pearson, Talima R; Engelthaler, David M.; Wagner, David M; Keim, Paul S; Schupp, James M.; Currie, Bart J.

In: Journal of Clinical Microbiology, Vol. 50, No. 6, 06.2012, p. 2059-2062.

Research output: Contribution to journalArticle

Kaestli, M, Richardson, LJ, Colman, RE, Tuanyok, A, Price, EP, Bowers, JR, Mayo, M, Kelley, E, Seymour, ML, Sarovich, DS, Pearson, TR, Engelthaler, DM, Wagner, DM, Keim, PS, Schupp, JM & Currie, BJ 2012, 'Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens', Journal of Clinical Microbiology, vol. 50, no. 6, pp. 2059-2062. https://doi.org/10.1128/JCM.06737-11
Kaestli, Mirjam ; Richardson, Leisha J. ; Colman, Rebecca E. ; Tuanyok, Apichai ; Price, Erin P. ; Bowers, Jolene R. ; Mayo, Mark ; Kelley, Erin ; Seymour, Meagan L. ; Sarovich, Derek S. ; Pearson, Talima R ; Engelthaler, David M. ; Wagner, David M ; Keim, Paul S ; Schupp, James M. ; Currie, Bart J. / Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens. In: Journal of Clinical Microbiology. 2012 ; Vol. 50, No. 6. pp. 2059-2062.
@article{d2d5d7020172442fbadb13db7c7d537d,
title = "Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens",
abstract = "Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.",
author = "Mirjam Kaestli and Richardson, {Leisha J.} and Colman, {Rebecca E.} and Apichai Tuanyok and Price, {Erin P.} and Bowers, {Jolene R.} and Mark Mayo and Erin Kelley and Seymour, {Meagan L.} and Sarovich, {Derek S.} and Pearson, {Talima R} and Engelthaler, {David M.} and Wagner, {David M} and Keim, {Paul S} and Schupp, {James M.} and Currie, {Bart J.}",
year = "2012",
month = "6",
doi = "10.1128/JCM.06737-11",
language = "English (US)",
volume = "50",
pages = "2059--2062",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens

AU - Kaestli, Mirjam

AU - Richardson, Leisha J.

AU - Colman, Rebecca E.

AU - Tuanyok, Apichai

AU - Price, Erin P.

AU - Bowers, Jolene R.

AU - Mayo, Mark

AU - Kelley, Erin

AU - Seymour, Meagan L.

AU - Sarovich, Derek S.

AU - Pearson, Talima R

AU - Engelthaler, David M.

AU - Wagner, David M

AU - Keim, Paul S

AU - Schupp, James M.

AU - Currie, Bart J.

PY - 2012/6

Y1 - 2012/6

N2 - Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

AB - Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

UR - http://www.scopus.com/inward/record.url?scp=84862075828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862075828&partnerID=8YFLogxK

U2 - 10.1128/JCM.06737-11

DO - 10.1128/JCM.06737-11

M3 - Article

VL - 50

SP - 2059

EP - 2062

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 6

ER -