Comparative phylogenomics and evolution of the brucellae reveal a path to virulence

Alice R. Wattam, Jeffrey T Foster, Shrinivasrao P. Mane, Stephen M Beckstrom-Sternberg, James M. Beckstrom-Sternberg, Allan W. Dickerman, Paul S Keim, Talima R Pearson, Maulik Shukla, Doyle V. Ward, Kelly P. Williams, Bruno W. Sobral, Renee M. Tsolis, Adrian M. Whatmore, David O'Callaghan

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Brucella species include important zoonotic pathogens that have a substantial impact on both agriculture and human health throughout the world. Brucellae are thought of as "stealth pathogens" that escape recognition by the host innate immune response, modulate the acquired immune response, and evade intracellular destruction. We analyzed the genome sequences of members of the family Brucellaceae to assess its evolutionary history from likely free-living soil-based progenitors into highly successful intracellular pathogens. Phylogenetic analysis split the genus into two groups: recently identified and early-dividing "atypical" strains and a highly conserved "classical" core clade containing the major pathogenic species. Lateral gene transfer events brought unique genomic regions into Brucella that differentiated them from Ochrobactrum and allowed the stepwise acquisition of virulence factors that include a type IV secretion system, a perosamine-based O antigen, and systems for sequestering metal ions that are absent in progenitors. Subsequent radiation within the core Brucella resulted in lineages that appear to have evolved within their preferred mammalian hosts, restricting their virulence to become stealth pathogens capable of causing long-term chronic infections.

Original languageEnglish (US)
Pages (from-to)920-930
Number of pages11
JournalJournal of Bacteriology
Volume196
Issue number5
DOIs
StatePublished - Mar 2014

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Brucella
Virulence
Brucellaceae
Ochrobactrum
Horizontal Gene Transfer
O Antigens
Zoonoses
Virulence Factors
Agriculture
Innate Immunity
Soil
History
Metals
Genome
Ions
Radiation
Infection

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Wattam, A. R., Foster, J. T., Mane, S. P., Beckstrom-Sternberg, S. M., Beckstrom-Sternberg, J. M., Dickerman, A. W., ... O'Callaghan, D. (2014). Comparative phylogenomics and evolution of the brucellae reveal a path to virulence. Journal of Bacteriology, 196(5), 920-930. https://doi.org/10.1128/JB.01091-13

Comparative phylogenomics and evolution of the brucellae reveal a path to virulence. / Wattam, Alice R.; Foster, Jeffrey T; Mane, Shrinivasrao P.; Beckstrom-Sternberg, Stephen M; Beckstrom-Sternberg, James M.; Dickerman, Allan W.; Keim, Paul S; Pearson, Talima R; Shukla, Maulik; Ward, Doyle V.; Williams, Kelly P.; Sobral, Bruno W.; Tsolis, Renee M.; Whatmore, Adrian M.; O'Callaghan, David.

In: Journal of Bacteriology, Vol. 196, No. 5, 03.2014, p. 920-930.

Research output: Contribution to journalArticle

Wattam, AR, Foster, JT, Mane, SP, Beckstrom-Sternberg, SM, Beckstrom-Sternberg, JM, Dickerman, AW, Keim, PS, Pearson, TR, Shukla, M, Ward, DV, Williams, KP, Sobral, BW, Tsolis, RM, Whatmore, AM & O'Callaghan, D 2014, 'Comparative phylogenomics and evolution of the brucellae reveal a path to virulence', Journal of Bacteriology, vol. 196, no. 5, pp. 920-930. https://doi.org/10.1128/JB.01091-13
Wattam, Alice R. ; Foster, Jeffrey T ; Mane, Shrinivasrao P. ; Beckstrom-Sternberg, Stephen M ; Beckstrom-Sternberg, James M. ; Dickerman, Allan W. ; Keim, Paul S ; Pearson, Talima R ; Shukla, Maulik ; Ward, Doyle V. ; Williams, Kelly P. ; Sobral, Bruno W. ; Tsolis, Renee M. ; Whatmore, Adrian M. ; O'Callaghan, David. / Comparative phylogenomics and evolution of the brucellae reveal a path to virulence. In: Journal of Bacteriology. 2014 ; Vol. 196, No. 5. pp. 920-930.
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