Cold stress-induced modulation of inflammatory responses and intracerebral cytokine mRNA expression in acute murine toxoplasmosis

Fernando P Monroy, Suman K. Banerjee, Taihun Duong, Hernan Aviles

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The effects of a physical stressor, cold water stress (CWS), within the central nervous system were investigated in the acute phase of infection with Toxoplasma gondii. Female BALB/c mice were subjected to CWS for 5 min each day for 8 days prior to oral infection with 20 cysts of the low virulent ME 49 strain. Animals were killed at 10-day intervals to detect inflammation, gliosis, and expression of intracerebral cytokine mRNAs. Zones of inflammation were detected by Nissl staining and gliosis by immunoreactivity to glial fibrillary acidic protein. Larger zones of inflammation and reactive astrogliosis were consistently observed in mice subjected to CWS and infected (CWS+INF) compared to control infected (INF) mice. Expression of interleukin (IL)-1β, IL-2, IL-12, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and inducible nitric oxide synthase (iNOS) were decreased in CWS+INF mice at 10 days postinoculation (PI), followed by a gradual increase after day 20 PI. This was in contrast to increased expression of these cytokines at 10 days PI in INF mice with a gradual decline thereafter. Inflammation and astrogliosis in CWS+INF mice were associated with an increased expression of IL-1β, IL-6, IL-12, and TNF-α between 20 and 30 days PI. These findings correlated with the continuous gene expression of tachyzoite surface antigen (SAG)-1 mRNA in CWS+INF mice compared to its sharp decline in INF mice after 20 days PI. These results suggest that CWS delays regulation and control of intracerebral Toxoplasma gondii during acute infection in BALB/c mice by decreasing the early expression of IFN-γ, IL-2, TNF-α, iNOS, IL-1β, and IL-12, while increasing the expression of IL-6, a counterregulatory cytokine.

Original languageEnglish (US)
Pages (from-to)878-886
Number of pages9
JournalJournal of Parasitology
Volume85
Issue number5
DOIs
StatePublished - Oct 1999
Externally publishedYes

Fingerprint

toxoplasmosis
Toxoplasmosis
cold stress
water stress
cold water
Dehydration
cytokines
inflammation
Cytokines
Messenger RNA
mice
tumor necrosis factors
interleukin-12
interleukin-1
tumor
Interleukin-12
Interleukin-1
Inflammation
Gliosis
nitric oxide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Cold stress-induced modulation of inflammatory responses and intracerebral cytokine mRNA expression in acute murine toxoplasmosis. / Monroy, Fernando P; Banerjee, Suman K.; Duong, Taihun; Aviles, Hernan.

In: Journal of Parasitology, Vol. 85, No. 5, 10.1999, p. 878-886.

Research output: Contribution to journalArticle

@article{722e01cf782c4e7b8250ea0962f13e24,
title = "Cold stress-induced modulation of inflammatory responses and intracerebral cytokine mRNA expression in acute murine toxoplasmosis",
abstract = "The effects of a physical stressor, cold water stress (CWS), within the central nervous system were investigated in the acute phase of infection with Toxoplasma gondii. Female BALB/c mice were subjected to CWS for 5 min each day for 8 days prior to oral infection with 20 cysts of the low virulent ME 49 strain. Animals were killed at 10-day intervals to detect inflammation, gliosis, and expression of intracerebral cytokine mRNAs. Zones of inflammation were detected by Nissl staining and gliosis by immunoreactivity to glial fibrillary acidic protein. Larger zones of inflammation and reactive astrogliosis were consistently observed in mice subjected to CWS and infected (CWS+INF) compared to control infected (INF) mice. Expression of interleukin (IL)-1β, IL-2, IL-12, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and inducible nitric oxide synthase (iNOS) were decreased in CWS+INF mice at 10 days postinoculation (PI), followed by a gradual increase after day 20 PI. This was in contrast to increased expression of these cytokines at 10 days PI in INF mice with a gradual decline thereafter. Inflammation and astrogliosis in CWS+INF mice were associated with an increased expression of IL-1β, IL-6, IL-12, and TNF-α between 20 and 30 days PI. These findings correlated with the continuous gene expression of tachyzoite surface antigen (SAG)-1 mRNA in CWS+INF mice compared to its sharp decline in INF mice after 20 days PI. These results suggest that CWS delays regulation and control of intracerebral Toxoplasma gondii during acute infection in BALB/c mice by decreasing the early expression of IFN-γ, IL-2, TNF-α, iNOS, IL-1β, and IL-12, while increasing the expression of IL-6, a counterregulatory cytokine.",
author = "Monroy, {Fernando P} and Banerjee, {Suman K.} and Taihun Duong and Hernan Aviles",
year = "1999",
month = "10",
doi = "10.2307/3285825",
language = "English (US)",
volume = "85",
pages = "878--886",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "5",

}

TY - JOUR

T1 - Cold stress-induced modulation of inflammatory responses and intracerebral cytokine mRNA expression in acute murine toxoplasmosis

AU - Monroy, Fernando P

AU - Banerjee, Suman K.

AU - Duong, Taihun

AU - Aviles, Hernan

PY - 1999/10

Y1 - 1999/10

N2 - The effects of a physical stressor, cold water stress (CWS), within the central nervous system were investigated in the acute phase of infection with Toxoplasma gondii. Female BALB/c mice were subjected to CWS for 5 min each day for 8 days prior to oral infection with 20 cysts of the low virulent ME 49 strain. Animals were killed at 10-day intervals to detect inflammation, gliosis, and expression of intracerebral cytokine mRNAs. Zones of inflammation were detected by Nissl staining and gliosis by immunoreactivity to glial fibrillary acidic protein. Larger zones of inflammation and reactive astrogliosis were consistently observed in mice subjected to CWS and infected (CWS+INF) compared to control infected (INF) mice. Expression of interleukin (IL)-1β, IL-2, IL-12, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and inducible nitric oxide synthase (iNOS) were decreased in CWS+INF mice at 10 days postinoculation (PI), followed by a gradual increase after day 20 PI. This was in contrast to increased expression of these cytokines at 10 days PI in INF mice with a gradual decline thereafter. Inflammation and astrogliosis in CWS+INF mice were associated with an increased expression of IL-1β, IL-6, IL-12, and TNF-α between 20 and 30 days PI. These findings correlated with the continuous gene expression of tachyzoite surface antigen (SAG)-1 mRNA in CWS+INF mice compared to its sharp decline in INF mice after 20 days PI. These results suggest that CWS delays regulation and control of intracerebral Toxoplasma gondii during acute infection in BALB/c mice by decreasing the early expression of IFN-γ, IL-2, TNF-α, iNOS, IL-1β, and IL-12, while increasing the expression of IL-6, a counterregulatory cytokine.

AB - The effects of a physical stressor, cold water stress (CWS), within the central nervous system were investigated in the acute phase of infection with Toxoplasma gondii. Female BALB/c mice were subjected to CWS for 5 min each day for 8 days prior to oral infection with 20 cysts of the low virulent ME 49 strain. Animals were killed at 10-day intervals to detect inflammation, gliosis, and expression of intracerebral cytokine mRNAs. Zones of inflammation were detected by Nissl staining and gliosis by immunoreactivity to glial fibrillary acidic protein. Larger zones of inflammation and reactive astrogliosis were consistently observed in mice subjected to CWS and infected (CWS+INF) compared to control infected (INF) mice. Expression of interleukin (IL)-1β, IL-2, IL-12, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and inducible nitric oxide synthase (iNOS) were decreased in CWS+INF mice at 10 days postinoculation (PI), followed by a gradual increase after day 20 PI. This was in contrast to increased expression of these cytokines at 10 days PI in INF mice with a gradual decline thereafter. Inflammation and astrogliosis in CWS+INF mice were associated with an increased expression of IL-1β, IL-6, IL-12, and TNF-α between 20 and 30 days PI. These findings correlated with the continuous gene expression of tachyzoite surface antigen (SAG)-1 mRNA in CWS+INF mice compared to its sharp decline in INF mice after 20 days PI. These results suggest that CWS delays regulation and control of intracerebral Toxoplasma gondii during acute infection in BALB/c mice by decreasing the early expression of IFN-γ, IL-2, TNF-α, iNOS, IL-1β, and IL-12, while increasing the expression of IL-6, a counterregulatory cytokine.

UR - http://www.scopus.com/inward/record.url?scp=0032729210&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032729210&partnerID=8YFLogxK

U2 - 10.2307/3285825

DO - 10.2307/3285825

M3 - Article

VL - 85

SP - 878

EP - 886

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 5

ER -