Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates

Paul J. Jackson, Eliza A. Walthers, Abdullahi S. Kalif, Kara L. Richmond, Debra M. Adair, Karen K. Hill, Cheryl R. Kuske, Gary L. Andersen, Kenneth H. Wilson, Martin E. Hugh-Jones, Paul S Keim

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

PCR analysis of 198 Bacillus anthracis isolates revealed a variable region of DNA sequence differing in length among the isolates. Five polymorphisms differed by the presence of two to six copies of the 12-bp tandem repeat 5'-CAATATCAACAA-3'. This variable-number tandem repeal (VNTR) region is located within a larger sequence containing one complete open reading frame thai encodes a putative 30-kDa protein. Length variation did not change the reading frame of the encoded protein and only changed the copy number of a 4-amino-acid sequence (QYQQ) from 2 to 6. The structure of the VNTR region suggests that these multiple repeats are generated by recombination or polymerase slippage. Protein structures predicted from the reverse-translated DNA sequence suggest that any structural changes in the encoded protein are confined in the region encoded by the VNTR sequence. Copy number differences in the VNTR region were used to define five different B. anthracis alleles. Characterization of 198 isolates revealed allele frequencies of 6.1, 17.7, 59.6, 5.6, and 11.1% sequentially from shorter to longer alleles. The high degree of polymorphism in the VNTR region provides a criterion for assigning isolates to five allelic categories. There is a correlation between categories and geographic distribution. Such molecular markers can be used to monitor the epidemiology of anthrax outbreaks in domestic and native herbivore populations.

Original languageEnglish (US)
Pages (from-to)1400-1405
Number of pages6
JournalApplied and Environmental Microbiology
Volume63
Issue number4
StatePublished - Apr 1997
Externally publishedYes

Fingerprint

Bacillus anthracis
Minisatellite Repeats
minisatellite repeats
allele
genetic polymorphism
protein
alleles
nucleotide sequences
anthrax
Proteins
tandem repeat sequences
proteins
Alleles
protein structure
polymorphism
Reading Frames
Anthrax
Herbivory
Tandem Repeat Sequences
gene frequency

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Jackson, P. J., Walthers, E. A., Kalif, A. S., Richmond, K. L., Adair, D. M., Hill, K. K., ... Keim, P. S. (1997). Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates. Applied and Environmental Microbiology, 63(4), 1400-1405.

Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates. / Jackson, Paul J.; Walthers, Eliza A.; Kalif, Abdullahi S.; Richmond, Kara L.; Adair, Debra M.; Hill, Karen K.; Kuske, Cheryl R.; Andersen, Gary L.; Wilson, Kenneth H.; Hugh-Jones, Martin E.; Keim, Paul S.

In: Applied and Environmental Microbiology, Vol. 63, No. 4, 04.1997, p. 1400-1405.

Research output: Contribution to journalArticle

Jackson, PJ, Walthers, EA, Kalif, AS, Richmond, KL, Adair, DM, Hill, KK, Kuske, CR, Andersen, GL, Wilson, KH, Hugh-Jones, ME & Keim, PS 1997, 'Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates', Applied and Environmental Microbiology, vol. 63, no. 4, pp. 1400-1405.
Jackson PJ, Walthers EA, Kalif AS, Richmond KL, Adair DM, Hill KK et al. Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates. Applied and Environmental Microbiology. 1997 Apr;63(4):1400-1405.
Jackson, Paul J. ; Walthers, Eliza A. ; Kalif, Abdullahi S. ; Richmond, Kara L. ; Adair, Debra M. ; Hill, Karen K. ; Kuske, Cheryl R. ; Andersen, Gary L. ; Wilson, Kenneth H. ; Hugh-Jones, Martin E. ; Keim, Paul S. / Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates. In: Applied and Environmental Microbiology. 1997 ; Vol. 63, No. 4. pp. 1400-1405.
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