Characterization of cytoplasmic and nuclear mutations affecting chlorophyll and chlorophyll-binding proteins during senescence in soybean

J. J. Guiamét, Egbert Schwartz, E. Pichersky, L. D. Noodén

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Abstract

Soybean plants (Glycine max [L.] Merr. cv Clark) carrying nuclear and cytoplasmic "stay-green" mutations, which affect senescence, were examined. Normally, the levels of chlorophyll (Chl) a and b decline during seedfill and the Chl a/b ratio decreases during late pod development in cv Clark. Plants homozygous for both the d1 and d2 recessive alleles, at two different nuclear loci, respectively, retained most (64%) of their Chl a and b and exhibited no change in their Chl a/b ratio. Combination of G (a dominant nuclear allele in a third locus causing only the seed coat to stay green during senescence) with d1d2 further inhibited the loss of Chl in the leaf. Whereas the thylakoid proteins seem to be degraded in normal Clark leaves during late pod development, they were not substantially diminished in d1d2 and Gd1d2 leaves. In plants carrying a cytoplasmic mutation, cytG, Chl declined in parallel with normal cv Clark; however, the cytG leaves had a much higher level of Chl b, and somewhat more Chl a, remaining at abscission, enough to color the leaves green. In cytG, most thylakoid proteins were degraded, but the Chl a/b-binding polypeptides of the light-harvesting complex in photosystem II (LHCII), and their associated Chl a and b molecules, were not. Thus, the combination of d1 and d2 causes broad preservation of the thylakoid proteins, whereas cytG appears to selectively preserve LHCII. The cytG mutation may be useful in elucidating the sequence of events involved in the degradation of LHCII proteins and their associated pigments during senescence.

Original languageEnglish (US)
Pages (from-to)227-231
Number of pages5
JournalPlant Physiology
Volume96
Issue number1
StatePublished - 1991
Externally publishedYes

Fingerprint

Chlorophyll Binding Proteins
Chlorophyll
Soybeans
soybeans
mutation
chlorophyll
Mutation
Thylakoids
Photosystem II Protein Complex
Light
light harvesting complex
Alleles
thylakoids
photosystem II
Proteins
chlorophyll a
chlorophyll b
chlorophyll binding proteins
leaves
Seeds

ASJC Scopus subject areas

  • Plant Science

Cite this

Characterization of cytoplasmic and nuclear mutations affecting chlorophyll and chlorophyll-binding proteins during senescence in soybean. / Guiamét, J. J.; Schwartz, Egbert; Pichersky, E.; Noodén, L. D.

In: Plant Physiology, Vol. 96, No. 1, 1991, p. 227-231.

Research output: Contribution to journalArticle

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