Abstract
Regulation of cell cycle progression occurs in part through the targeted degradation of both activating and inhibitory subunits of the cyclin- dependent kinases. During G1, CDC4, encoding a WD-40 repeat protein, and CDC34, encoding a ubiquitin-conjugating enzyme, are involved in the destruction of these regulators. Here we describe evidence indicating that CDC53 also is involved in this process. Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo. Cdc53p defines a large family of proteins found in yeasts, nematodes, and humans whose molecular functions are uncharacterized. These results suggest a role for this family of proteins in regulating cell cycle proliferation through protein degradation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6634-6643 |
| Number of pages | 10 |
| Journal | Molecular and Cellular Biology |
| Volume | 16 |
| Issue number | 12 |
| State | Published - 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Cdc53p acts in concert with cdc4p and cdc34p to control the G1-to-S- phase transition and identifies a conserved family of proteins. / Mathias, Neal; Johnson, Stephen L.; Winey, Mark; Adams, Alison; Goetsch, Loretta; Pringle, John R.; Byers, Breck; Goebl, Mark G.
In: Molecular and Cellular Biology, Vol. 16, No. 12, 1996, p. 6634-6643.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cdc53p acts in concert with cdc4p and cdc34p to control the G1-to-S- phase transition and identifies a conserved family of proteins
AU - Mathias, Neal
AU - Johnson, Stephen L.
AU - Winey, Mark
AU - Adams, Alison
AU - Goetsch, Loretta
AU - Pringle, John R.
AU - Byers, Breck
AU - Goebl, Mark G.
PY - 1996
Y1 - 1996
N2 - Regulation of cell cycle progression occurs in part through the targeted degradation of both activating and inhibitory subunits of the cyclin- dependent kinases. During G1, CDC4, encoding a WD-40 repeat protein, and CDC34, encoding a ubiquitin-conjugating enzyme, are involved in the destruction of these regulators. Here we describe evidence indicating that CDC53 also is involved in this process. Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo. Cdc53p defines a large family of proteins found in yeasts, nematodes, and humans whose molecular functions are uncharacterized. These results suggest a role for this family of proteins in regulating cell cycle proliferation through protein degradation.
AB - Regulation of cell cycle progression occurs in part through the targeted degradation of both activating and inhibitory subunits of the cyclin- dependent kinases. During G1, CDC4, encoding a WD-40 repeat protein, and CDC34, encoding a ubiquitin-conjugating enzyme, are involved in the destruction of these regulators. Here we describe evidence indicating that CDC53 also is involved in this process. Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo. Cdc53p defines a large family of proteins found in yeasts, nematodes, and humans whose molecular functions are uncharacterized. These results suggest a role for this family of proteins in regulating cell cycle proliferation through protein degradation.
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M3 - Article
VL - 16
SP - 6634
EP - 6643
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 12
ER -