Carbohydrate-binding plasma proteins from the gastropod Biomphalaria glabrata

Strain specificity and the effects of trematode infection

Fernando P Monroy, Lynn A. Hertel, Eric S. Loker

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

As lectins are believed to mediate non-self-recognition in molluscs, carbohydrate-binding proteins (CBP) from the circulating plasma of the gastropod Biomphalaria glabrata were harvested by affinity chromatography using six different monosaccharides as ligands. Pools of plasma were derived from B. glabrata of either the M line strain, which is susceptible to infection with the PR1 strain of the digenetic trematode Schistosoma mansoni, or from the 13-16-R1 strain, which is resistant to infection. For each strain, plasma was obtained from control snails and from snails exposed to infection 1 or 8 days previously with S. mansoni or the related digenean, Echinostoma paraensei, which is able to develop in either host strain. For control snails, only minor interstrain differences were noted. In M line snails exposed 8 days previously to either parasite, marked changes in CBP populations were observed. Only E. paraensei infections produced comparable alterations in 13-16-R1 snails. The most conspicuous changes noted were the increased production of 80-120 kDa CBP in both strains, 150-210 kDa in 13-16-R1 snails, and 190-210 kDa in M line snails. The results demonstrate 1) interstrain differences in CBP, particularly following exposure to trematodes; 2) that infection provokes increased production and diversity of CBP that bind with greater affinity to the columns; and 3) that snails of the same strain respond differently to the two parasites used.

Original languageEnglish (US)
Pages (from-to)355-366
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume16
Issue number5
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Trematode Infections
Biomphalaria
Gastropoda
Snails
Blood Proteins
Infection
Schistosoma mansoni
Parasites
Echinostoma
saccharide-binding proteins
Monosaccharides
Mollusca
Affinity Chromatography
Lectins
Ligands

Keywords

  • Biomphalaria glabrata
  • Comparative immunobiology
  • Digenetic trematodes
  • Echinostoma paraensei
  • Hemolymph proteins
  • Lectins
  • Molluscs
  • Schistosoma mansoni

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

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title = "Carbohydrate-binding plasma proteins from the gastropod Biomphalaria glabrata: Strain specificity and the effects of trematode infection",
abstract = "As lectins are believed to mediate non-self-recognition in molluscs, carbohydrate-binding proteins (CBP) from the circulating plasma of the gastropod Biomphalaria glabrata were harvested by affinity chromatography using six different monosaccharides as ligands. Pools of plasma were derived from B. glabrata of either the M line strain, which is susceptible to infection with the PR1 strain of the digenetic trematode Schistosoma mansoni, or from the 13-16-R1 strain, which is resistant to infection. For each strain, plasma was obtained from control snails and from snails exposed to infection 1 or 8 days previously with S. mansoni or the related digenean, Echinostoma paraensei, which is able to develop in either host strain. For control snails, only minor interstrain differences were noted. In M line snails exposed 8 days previously to either parasite, marked changes in CBP populations were observed. Only E. paraensei infections produced comparable alterations in 13-16-R1 snails. The most conspicuous changes noted were the increased production of 80-120 kDa CBP in both strains, 150-210 kDa in 13-16-R1 snails, and 190-210 kDa in M line snails. The results demonstrate 1) interstrain differences in CBP, particularly following exposure to trematodes; 2) that infection provokes increased production and diversity of CBP that bind with greater affinity to the columns; and 3) that snails of the same strain respond differently to the two parasites used.",
keywords = "Biomphalaria glabrata, Comparative immunobiology, Digenetic trematodes, Echinostoma paraensei, Hemolymph proteins, Lectins, Molluscs, Schistosoma mansoni",
author = "Monroy, {Fernando P} and Hertel, {Lynn A.} and Loker, {Eric S.}",
year = "1992",
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journal = "Developmental and Comparative Immunology",
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TY - JOUR

T1 - Carbohydrate-binding plasma proteins from the gastropod Biomphalaria glabrata

T2 - Strain specificity and the effects of trematode infection

AU - Monroy, Fernando P

AU - Hertel, Lynn A.

AU - Loker, Eric S.

PY - 1992

Y1 - 1992

N2 - As lectins are believed to mediate non-self-recognition in molluscs, carbohydrate-binding proteins (CBP) from the circulating plasma of the gastropod Biomphalaria glabrata were harvested by affinity chromatography using six different monosaccharides as ligands. Pools of plasma were derived from B. glabrata of either the M line strain, which is susceptible to infection with the PR1 strain of the digenetic trematode Schistosoma mansoni, or from the 13-16-R1 strain, which is resistant to infection. For each strain, plasma was obtained from control snails and from snails exposed to infection 1 or 8 days previously with S. mansoni or the related digenean, Echinostoma paraensei, which is able to develop in either host strain. For control snails, only minor interstrain differences were noted. In M line snails exposed 8 days previously to either parasite, marked changes in CBP populations were observed. Only E. paraensei infections produced comparable alterations in 13-16-R1 snails. The most conspicuous changes noted were the increased production of 80-120 kDa CBP in both strains, 150-210 kDa in 13-16-R1 snails, and 190-210 kDa in M line snails. The results demonstrate 1) interstrain differences in CBP, particularly following exposure to trematodes; 2) that infection provokes increased production and diversity of CBP that bind with greater affinity to the columns; and 3) that snails of the same strain respond differently to the two parasites used.

AB - As lectins are believed to mediate non-self-recognition in molluscs, carbohydrate-binding proteins (CBP) from the circulating plasma of the gastropod Biomphalaria glabrata were harvested by affinity chromatography using six different monosaccharides as ligands. Pools of plasma were derived from B. glabrata of either the M line strain, which is susceptible to infection with the PR1 strain of the digenetic trematode Schistosoma mansoni, or from the 13-16-R1 strain, which is resistant to infection. For each strain, plasma was obtained from control snails and from snails exposed to infection 1 or 8 days previously with S. mansoni or the related digenean, Echinostoma paraensei, which is able to develop in either host strain. For control snails, only minor interstrain differences were noted. In M line snails exposed 8 days previously to either parasite, marked changes in CBP populations were observed. Only E. paraensei infections produced comparable alterations in 13-16-R1 snails. The most conspicuous changes noted were the increased production of 80-120 kDa CBP in both strains, 150-210 kDa in 13-16-R1 snails, and 190-210 kDa in M line snails. The results demonstrate 1) interstrain differences in CBP, particularly following exposure to trematodes; 2) that infection provokes increased production and diversity of CBP that bind with greater affinity to the columns; and 3) that snails of the same strain respond differently to the two parasites used.

KW - Biomphalaria glabrata

KW - Comparative immunobiology

KW - Digenetic trematodes

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KW - Schistosoma mansoni

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U2 - 10.1016/0145-305X(92)90038-E

DO - 10.1016/0145-305X(92)90038-E

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VL - 16

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EP - 366

JO - Developmental and Comparative Immunology

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SN - 0145-305X

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ER -