Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources

Birgit De Smet, Mark Mayo, Charlotte Peeters, James E A Zlosnik, Theodore Spilker, Trevor J. Hird, John J. Li Puma, Timothy J. Kidd, Mirjam Kaestli, Jennifer L. Ginther, David M Wagner, Paul S Keim, Scott C. Bell, Jan A. Jacobs, Bart J. Currie, Peter Vandamme

Research output: Contribution to journalArticle

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Abstract

Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9%, respectively) and towards established Bcc species (4.0 and 3.9%, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156<sup>T</sup> (=CCUG 65686<sup>T</sup>) and Burkholderia territorii sp. nov. with type strain LMG 28158<sup>T</sup> (=CCUG 65687<sup>T</sup>) are proposed for Bcc B and Bcc L bacteria, respectively.

Original languageEnglish (US)
Pages (from-to)2265-2271
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number7
DOIs
StatePublished - Jul 1 2015

Fingerprint

Burkholderia cepacia complex
Burkholderia
species complex
new species
bacterium
gene
ester
nucleotide sequences
analysis
Sequence Analysis
fatty acid
divergence
Burkholderia pseudomallei
Northern Territory
DNA
Melioidosis
Bacteria
N-acetylglucosamine
Maltose
Acetylglucosamine

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources. / De Smet, Birgit; Mayo, Mark; Peeters, Charlotte; Zlosnik, James E A; Spilker, Theodore; Hird, Trevor J.; Li Puma, John J.; Kidd, Timothy J.; Kaestli, Mirjam; Ginther, Jennifer L.; Wagner, David M; Keim, Paul S; Bell, Scott C.; Jacobs, Jan A.; Currie, Bart J.; Vandamme, Peter.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 7, 01.07.2015, p. 2265-2271.

Research output: Contribution to journalArticle

De Smet, B, Mayo, M, Peeters, C, Zlosnik, JEA, Spilker, T, Hird, TJ, Li Puma, JJ, Kidd, TJ, Kaestli, M, Ginther, JL, Wagner, DM, Keim, PS, Bell, SC, Jacobs, JA, Currie, BJ & Vandamme, P 2015, 'Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources', International Journal of Systematic and Evolutionary Microbiology, vol. 65, no. 7, pp. 2265-2271. https://doi.org/10.1099/ijs.0.000251
De Smet, Birgit ; Mayo, Mark ; Peeters, Charlotte ; Zlosnik, James E A ; Spilker, Theodore ; Hird, Trevor J. ; Li Puma, John J. ; Kidd, Timothy J. ; Kaestli, Mirjam ; Ginther, Jennifer L. ; Wagner, David M ; Keim, Paul S ; Bell, Scott C. ; Jacobs, Jan A. ; Currie, Bart J. ; Vandamme, Peter. / Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65, No. 7. pp. 2265-2271.
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abstract = "Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9{\%}, respectively) and towards established Bcc species (4.0 and 3.9{\%}, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156T (=CCUG 65686T) and Burkholderia territorii sp. nov. with type strain LMG 28158T (=CCUG 65687T) are proposed for Bcc B and Bcc L bacteria, respectively.",
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T1 - Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources

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AU - Mayo, Mark

AU - Peeters, Charlotte

AU - Zlosnik, James E A

AU - Spilker, Theodore

AU - Hird, Trevor J.

AU - Li Puma, John J.

AU - Kidd, Timothy J.

AU - Kaestli, Mirjam

AU - Ginther, Jennifer L.

AU - Wagner, David M

AU - Keim, Paul S

AU - Bell, Scott C.

AU - Jacobs, Jan A.

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AB - Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9%, respectively) and towards established Bcc species (4.0 and 3.9%, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156T (=CCUG 65686T) and Burkholderia territorii sp. nov. with type strain LMG 28158T (=CCUG 65687T) are proposed for Bcc B and Bcc L bacteria, respectively.

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