Burkholderia pseudomallei isolates from sarawak, malaysian borneo, are predominantly susceptible to aminoglycosides and macrolides

Yuwana Podin, Derek S. Sarovich, Erin P. Price, Mirjam Kaestli, Mark Mayo, Kingching Hii, Ngian HieUng, Seechang Wong, Ingtien Wong, Jinshyan Wong, Anand Mohan, Monghow Ooi, Temlom Fam, Jack Wong, Apichai Tuanyok, Paul S Keim, Philip M. Giffard, Bart J. Currie

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Melioidosis is a potentially fatal disease caused by the saprophytic bacterium Burkholderia pseudomallei. Resistance to gentamicin is generally a hallmark of B. pseudomallei, and gentamicin is a selective agent in media used for diagnosis of melioidosis. In this study, we determined the prevalence and mechanism of gentamicin susceptibility found in B. pseudomallei isolates from Sarawak, Malaysian Borneo. We performed multilocus sequence typing and antibiotic susceptibility testing on 44 B. pseudomallei clinical isolates from melioidosis patients in Sarawak district hospitals. Whole-genome sequencing was used to identify the mechanism of gentamicin susceptibility. A novel allelic-specific PCR was designed to differentiate gentamicin-sensitive isolates from wild-type B. pseudomallei. A reversion assay was performed to confirm the involvement of this mechanism in gentamicin susceptibility. A substantial proportion (86%) of B. pseudomallei clinical isolates in Sarawak, Malaysian Borneo, were found to be susceptible to the aminoglycoside gentamicin, a rare occurrence in other regions where B. pseudomallei is endemic. Gentamicin sensitivity was restricted to genetically related strains belonging to sequence type 881 or its single-locus variant, sequence type 997. Whole-genome sequencing identified a novel nonsynonymous mutation within amrB, encoding an essential component of the AmrAB-OprA multidrug efflux pump. We confirmed the role of this mutation in conferring aminoglycoside and macrolide sensitivity by reversion of this mutation to the wild-type sequence. Our study demonstrates that alternative B. pseudomallei selective media without gentamicin are needed for accurate melioidosis laboratory diagnosis in Sarawak. This finding may also have implications for environmental sampling of other locations to test for B. pseudomallei endemicity.

Original languageEnglish (US)
Pages (from-to)162-166
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume58
Issue number1
DOIs
StatePublished - Jan 2014

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Borneo
Burkholderia pseudomallei
Malaysia
Macrolides
Aminoglycosides
Gentamicins
Melioidosis
Mutation
Genome
Multilocus Sequence Typing
District Hospitals
Clinical Laboratory Techniques

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Burkholderia pseudomallei isolates from sarawak, malaysian borneo, are predominantly susceptible to aminoglycosides and macrolides. / Podin, Yuwana; Sarovich, Derek S.; Price, Erin P.; Kaestli, Mirjam; Mayo, Mark; Hii, Kingching; HieUng, Ngian; Wong, Seechang; Wong, Ingtien; Wong, Jinshyan; Mohan, Anand; Ooi, Monghow; Fam, Temlom; Wong, Jack; Tuanyok, Apichai; Keim, Paul S; Giffard, Philip M.; Currie, Bart J.

In: Antimicrobial Agents and Chemotherapy, Vol. 58, No. 1, 01.2014, p. 162-166.

Research output: Contribution to journalArticle

Podin, Y, Sarovich, DS, Price, EP, Kaestli, M, Mayo, M, Hii, K, HieUng, N, Wong, S, Wong, I, Wong, J, Mohan, A, Ooi, M, Fam, T, Wong, J, Tuanyok, A, Keim, PS, Giffard, PM & Currie, BJ 2014, 'Burkholderia pseudomallei isolates from sarawak, malaysian borneo, are predominantly susceptible to aminoglycosides and macrolides', Antimicrobial Agents and Chemotherapy, vol. 58, no. 1, pp. 162-166. https://doi.org/10.1128/AAC.01842-13
Podin, Yuwana ; Sarovich, Derek S. ; Price, Erin P. ; Kaestli, Mirjam ; Mayo, Mark ; Hii, Kingching ; HieUng, Ngian ; Wong, Seechang ; Wong, Ingtien ; Wong, Jinshyan ; Mohan, Anand ; Ooi, Monghow ; Fam, Temlom ; Wong, Jack ; Tuanyok, Apichai ; Keim, Paul S ; Giffard, Philip M. ; Currie, Bart J. / Burkholderia pseudomallei isolates from sarawak, malaysian borneo, are predominantly susceptible to aminoglycosides and macrolides. In: Antimicrobial Agents and Chemotherapy. 2014 ; Vol. 58, No. 1. pp. 162-166.
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abstract = "Melioidosis is a potentially fatal disease caused by the saprophytic bacterium Burkholderia pseudomallei. Resistance to gentamicin is generally a hallmark of B. pseudomallei, and gentamicin is a selective agent in media used for diagnosis of melioidosis. In this study, we determined the prevalence and mechanism of gentamicin susceptibility found in B. pseudomallei isolates from Sarawak, Malaysian Borneo. We performed multilocus sequence typing and antibiotic susceptibility testing on 44 B. pseudomallei clinical isolates from melioidosis patients in Sarawak district hospitals. Whole-genome sequencing was used to identify the mechanism of gentamicin susceptibility. A novel allelic-specific PCR was designed to differentiate gentamicin-sensitive isolates from wild-type B. pseudomallei. A reversion assay was performed to confirm the involvement of this mechanism in gentamicin susceptibility. A substantial proportion (86{\%}) of B. pseudomallei clinical isolates in Sarawak, Malaysian Borneo, were found to be susceptible to the aminoglycoside gentamicin, a rare occurrence in other regions where B. pseudomallei is endemic. Gentamicin sensitivity was restricted to genetically related strains belonging to sequence type 881 or its single-locus variant, sequence type 997. Whole-genome sequencing identified a novel nonsynonymous mutation within amrB, encoding an essential component of the AmrAB-OprA multidrug efflux pump. We confirmed the role of this mutation in conferring aminoglycoside and macrolide sensitivity by reversion of this mutation to the wild-type sequence. Our study demonstrates that alternative B. pseudomallei selective media without gentamicin are needed for accurate melioidosis laboratory diagnosis in Sarawak. This finding may also have implications for environmental sampling of other locations to test for B. pseudomallei endemicity.",
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