Burkholderia humptydooensis sp. nov., a new species related to Burkholderia thailandensis and the fifth member of the Burkholderia pseudomallei complex

Apichai Tuanyok, Mark Mayo, Holger Scholz, Carina M. Hall, Christopher J. Allender, Mirjam Kaestli, Jennifer Ginther, Senanu Spring-Pearson, Molly C. Bollig, Joshua K. Stone, Erik W. Settles, Joseph D. Busch, Lindsay Sidak-Loftis, Jason W. Sahl, Astrid Thomas, Lisa Kreutzer, Enrico Georgi, Jay E. Gee, Richard A. Bowen, Jason T. LadnerSean Lovett, Galina Koroleva, Gustavo Palacios, David M Wagner, Bart J. Currie, Paul S Keim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43T, MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish B. humptydooensis sp. nov. from closely related species. With matrix-assisted laser desorption ionization-time of flight analysis, all isolates grouped together in a cluster separate from other Burkholderia spp. 16S rRNA and recA sequence analyses demonstrated phylogenetic placement for B. humptydooensis sp. nov. in a novel clade within the B. pseudomallei group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340 B. pseudomallei strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both B. thailandensis and B. pseudomallei, based on whole-genome sequences, also confirmed B. humptydooensis sp. nov. as a novel Burkholderia species within the B. pseudomallei complex. Molecular analyses clearly demonstrated that strains MSMB43T, MSMB121, and MSMB122 belong to a novel Burkholderia species for which the name Burkholderia humptydooensis sp. nov. is proposed, with the type strain MSMB43T (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers CP013380 to CP013382).

Original languageEnglish (US)
Article numbere02802-16
JournalApplied and Environmental Microbiology
Volume83
Issue number5
DOIs
StatePublished - 2017

Fingerprint

Burkholderia thailandensis
Burkholderia pseudomallei
Burkholderia
genome
new species
Multilocus Sequence Typing
agar
ester
desorption
ionization
Genome
fatty acid
microorganism
laser
phylogenetics
bacterium
matrix
Water Wells
analysis
Nucleic Acid Databases

Keywords

  • Burkholderia humptydooensis sp. nov.
  • Burkholderia pseudomallei complex
  • MSMB43

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Burkholderia humptydooensis sp. nov., a new species related to Burkholderia thailandensis and the fifth member of the Burkholderia pseudomallei complex. / Tuanyok, Apichai; Mayo, Mark; Scholz, Holger; Hall, Carina M.; Allender, Christopher J.; Kaestli, Mirjam; Ginther, Jennifer; Spring-Pearson, Senanu; Bollig, Molly C.; Stone, Joshua K.; Settles, Erik W.; Busch, Joseph D.; Sidak-Loftis, Lindsay; Sahl, Jason W.; Thomas, Astrid; Kreutzer, Lisa; Georgi, Enrico; Gee, Jay E.; Bowen, Richard A.; Ladner, Jason T.; Lovett, Sean; Koroleva, Galina; Palacios, Gustavo; Wagner, David M; Currie, Bart J.; Keim, Paul S.

In: Applied and Environmental Microbiology, Vol. 83, No. 5, e02802-16, 2017.

Research output: Contribution to journalArticle

Tuanyok, A, Mayo, M, Scholz, H, Hall, CM, Allender, CJ, Kaestli, M, Ginther, J, Spring-Pearson, S, Bollig, MC, Stone, JK, Settles, EW, Busch, JD, Sidak-Loftis, L, Sahl, JW, Thomas, A, Kreutzer, L, Georgi, E, Gee, JE, Bowen, RA, Ladner, JT, Lovett, S, Koroleva, G, Palacios, G, Wagner, DM, Currie, BJ & Keim, PS 2017, 'Burkholderia humptydooensis sp. nov., a new species related to Burkholderia thailandensis and the fifth member of the Burkholderia pseudomallei complex', Applied and Environmental Microbiology, vol. 83, no. 5, e02802-16. https://doi.org/10.1128/AEM.02802-16
Tuanyok, Apichai ; Mayo, Mark ; Scholz, Holger ; Hall, Carina M. ; Allender, Christopher J. ; Kaestli, Mirjam ; Ginther, Jennifer ; Spring-Pearson, Senanu ; Bollig, Molly C. ; Stone, Joshua K. ; Settles, Erik W. ; Busch, Joseph D. ; Sidak-Loftis, Lindsay ; Sahl, Jason W. ; Thomas, Astrid ; Kreutzer, Lisa ; Georgi, Enrico ; Gee, Jay E. ; Bowen, Richard A. ; Ladner, Jason T. ; Lovett, Sean ; Koroleva, Galina ; Palacios, Gustavo ; Wagner, David M ; Currie, Bart J. ; Keim, Paul S. / Burkholderia humptydooensis sp. nov., a new species related to Burkholderia thailandensis and the fifth member of the Burkholderia pseudomallei complex. In: Applied and Environmental Microbiology. 2017 ; Vol. 83, No. 5.
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abstract = "During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43T, MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish B. humptydooensis sp. nov. from closely related species. With matrix-assisted laser desorption ionization-time of flight analysis, all isolates grouped together in a cluster separate from other Burkholderia spp. 16S rRNA and recA sequence analyses demonstrated phylogenetic placement for B. humptydooensis sp. nov. in a novel clade within the B. pseudomallei group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340 B. pseudomallei strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both B. thailandensis and B. pseudomallei, based on whole-genome sequences, also confirmed B. humptydooensis sp. nov. as a novel Burkholderia species within the B. pseudomallei complex. Molecular analyses clearly demonstrated that strains MSMB43T, MSMB121, and MSMB122 belong to a novel Burkholderia species for which the name Burkholderia humptydooensis sp. nov. is proposed, with the type strain MSMB43T (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers CP013380 to CP013382).",
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AU - Tuanyok, Apichai

AU - Mayo, Mark

AU - Scholz, Holger

AU - Hall, Carina M.

AU - Allender, Christopher J.

AU - Kaestli, Mirjam

AU - Ginther, Jennifer

AU - Spring-Pearson, Senanu

AU - Bollig, Molly C.

AU - Stone, Joshua K.

AU - Settles, Erik W.

AU - Busch, Joseph D.

AU - Sidak-Loftis, Lindsay

AU - Sahl, Jason W.

AU - Thomas, Astrid

AU - Kreutzer, Lisa

AU - Georgi, Enrico

AU - Gee, Jay E.

AU - Bowen, Richard A.

AU - Ladner, Jason T.

AU - Lovett, Sean

AU - Koroleva, Galina

AU - Palacios, Gustavo

AU - Wagner, David M

AU - Currie, Bart J.

AU - Keim, Paul S

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N2 - During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43T, MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish B. humptydooensis sp. nov. from closely related species. With matrix-assisted laser desorption ionization-time of flight analysis, all isolates grouped together in a cluster separate from other Burkholderia spp. 16S rRNA and recA sequence analyses demonstrated phylogenetic placement for B. humptydooensis sp. nov. in a novel clade within the B. pseudomallei group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340 B. pseudomallei strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both B. thailandensis and B. pseudomallei, based on whole-genome sequences, also confirmed B. humptydooensis sp. nov. as a novel Burkholderia species within the B. pseudomallei complex. Molecular analyses clearly demonstrated that strains MSMB43T, MSMB121, and MSMB122 belong to a novel Burkholderia species for which the name Burkholderia humptydooensis sp. nov. is proposed, with the type strain MSMB43T (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers CP013380 to CP013382).

AB - During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43T, MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish B. humptydooensis sp. nov. from closely related species. With matrix-assisted laser desorption ionization-time of flight analysis, all isolates grouped together in a cluster separate from other Burkholderia spp. 16S rRNA and recA sequence analyses demonstrated phylogenetic placement for B. humptydooensis sp. nov. in a novel clade within the B. pseudomallei group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340 B. pseudomallei strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both B. thailandensis and B. pseudomallei, based on whole-genome sequences, also confirmed B. humptydooensis sp. nov. as a novel Burkholderia species within the B. pseudomallei complex. Molecular analyses clearly demonstrated that strains MSMB43T, MSMB121, and MSMB122 belong to a novel Burkholderia species for which the name Burkholderia humptydooensis sp. nov. is proposed, with the type strain MSMB43T (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers CP013380 to CP013382).

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