BurkDiff: A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei

Jolene R. Bowers, David M. Engelthaler, Jennifer L. Ginther, Talima Pearson, Sharon J. Peacock, Apichai Tuanyok, David M. Wagner, Bart J. Currie, Paul S. Keim

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.

Original languageEnglish (US)
Article numbere15413
JournalPLoS ONE
Volume5
Issue number11
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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