BurkDiff

A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei

Jolene R. Bowers, David M. Engelthaler, Jennifer L. Ginther, Talima Pearson, Sharon J. Peacock, Apichai Tuanyok, David M. Wagner, Bart J. Currie, Paul S. Keim

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.

Original languageEnglish (US)
Article numbere15413
JournalPLoS One
Volume5
Issue number11
DOIs
StatePublished - 2010

Fingerprint

Malleus
Burkholderia pseudomallei
nontarget organisms
Real-Time Polymerase Chain Reaction
Assays
quantitative polymerase chain reaction
assays
cross reaction
Screening
Genes
screening
Single Nucleotide Polymorphism
genome
Genome
Sensitivity and Specificity
methodology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

BurkDiff : A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei. / Bowers, Jolene R.; Engelthaler, David M.; Ginther, Jennifer L.; Pearson, Talima; Peacock, Sharon J.; Tuanyok, Apichai; Wagner, David M.; Currie, Bart J.; Keim, Paul S.

In: PLoS One, Vol. 5, No. 11, e15413, 2010.

Research output: Contribution to journalArticle

Bowers, Jolene R. ; Engelthaler, David M. ; Ginther, Jennifer L. ; Pearson, Talima ; Peacock, Sharon J. ; Tuanyok, Apichai ; Wagner, David M. ; Currie, Bart J. ; Keim, Paul S. / BurkDiff : A real-time PCR allelic discrimination assay for burkholderia Pseudomallei and B. mallei. In: PLoS One. 2010 ; Vol. 5, No. 11.
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