Biomphalaria glabrata Hemolymph Lectins

Binding to Bacteria, Mammalian Erythrocytes, and to Sporocysts and Rediae of Echinostoma paraensei

Lynn A. Hertel, Stephen A. Stricker, Fernando P Monroy, Wade D. Wilson, Eric S. Loker

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Polyclonal antibodies were raised in rabbits to two groups of diffusely staining M line Biomphalaria glabrata plasma polypeptides, of 150-210 and 70-120 kDa, designated as Group 1 molecules (G1M) and group 2 molecules (G2M), respectively. G1M and G2M are known to increase in abundance and to become more diverse following infection of B. glabrata with the digenetic trematode Echinostoma paraensei. These antibodies were used in conjunction with immunoblotting and slot blotting procedures to document binding of G1M/G2M from plasma of unexposed control snails (C plasma) or plasma from snails with 8-day infections of E. paraensei (I plasma) to various targets. Binding of G1M/G2M to both gram-positive and gram-negative bacteria, human A, B, and O and rabbit erythrocytes, and to sporocysts and rediae of E. paraensei was documented. Immunoblots revealed that erythrocytes are bound by a particular G2M band of 100-120 kDa present in I plasma that is in low concentration or lacking in C plasma. This explains previous results indicating that I plasma agglutinates all 4 erythrocytes examined whereas C plasma agglutinates only rabbit erythrocytes. More G1M/G2M binding to sporocysts occurred if I plasma rather than C plasma was used for incubations. Also, monosaccharide inhibition of G1M/G2M binding to sporocysts was observed in I plasma but not in C plasma. The results indicate that infection with E. paraensei induces production by B. glabrata of unique plasma polypeptides and that molecules present in I plasma can bind to the surfaces of non-self objects including E. paraensei larvae in a lectin-like fashion.

Original languageEnglish (US)
Pages (from-to)52-61
Number of pages10
JournalJournal of Invertebrate Pathology
Volume64
Issue number1
DOIs
StatePublished - Jul 1994
Externally publishedYes

Fingerprint

sporocysts (Trematoda)
Echinostoma
Biomphalaria glabrata
lectins
hemolymph
erythrocytes
plasma
bacterium
rabbits
bacteria
polypeptides
infection
mollusc control
monosaccharides
immunoblotting
polyclonal antibodies
Trematoda
Gram-negative bacteria
snails
antibodies

Keywords

  • Biomphalaria glabrata
  • comparative immunobiology
  • Echinostoma paraensei
  • Gastropoda
  • lectins
  • Mollusca
  • non-self-recognition
  • snail-trematode interactions
  • Trematoda

ASJC Scopus subject areas

  • Insect Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Biomphalaria glabrata Hemolymph Lectins : Binding to Bacteria, Mammalian Erythrocytes, and to Sporocysts and Rediae of Echinostoma paraensei. / Hertel, Lynn A.; Stricker, Stephen A.; Monroy, Fernando P; Wilson, Wade D.; Loker, Eric S.

In: Journal of Invertebrate Pathology, Vol. 64, No. 1, 07.1994, p. 52-61.

Research output: Contribution to journalArticle

@article{8cead1cf09b4465d929508103f86c140,
title = "Biomphalaria glabrata Hemolymph Lectins: Binding to Bacteria, Mammalian Erythrocytes, and to Sporocysts and Rediae of Echinostoma paraensei",
abstract = "Polyclonal antibodies were raised in rabbits to two groups of diffusely staining M line Biomphalaria glabrata plasma polypeptides, of 150-210 and 70-120 kDa, designated as Group 1 molecules (G1M) and group 2 molecules (G2M), respectively. G1M and G2M are known to increase in abundance and to become more diverse following infection of B. glabrata with the digenetic trematode Echinostoma paraensei. These antibodies were used in conjunction with immunoblotting and slot blotting procedures to document binding of G1M/G2M from plasma of unexposed control snails (C plasma) or plasma from snails with 8-day infections of E. paraensei (I plasma) to various targets. Binding of G1M/G2M to both gram-positive and gram-negative bacteria, human A, B, and O and rabbit erythrocytes, and to sporocysts and rediae of E. paraensei was documented. Immunoblots revealed that erythrocytes are bound by a particular G2M band of 100-120 kDa present in I plasma that is in low concentration or lacking in C plasma. This explains previous results indicating that I plasma agglutinates all 4 erythrocytes examined whereas C plasma agglutinates only rabbit erythrocytes. More G1M/G2M binding to sporocysts occurred if I plasma rather than C plasma was used for incubations. Also, monosaccharide inhibition of G1M/G2M binding to sporocysts was observed in I plasma but not in C plasma. The results indicate that infection with E. paraensei induces production by B. glabrata of unique plasma polypeptides and that molecules present in I plasma can bind to the surfaces of non-self objects including E. paraensei larvae in a lectin-like fashion.",
keywords = "Biomphalaria glabrata, comparative immunobiology, Echinostoma paraensei, Gastropoda, lectins, Mollusca, non-self-recognition, snail-trematode interactions, Trematoda",
author = "Hertel, {Lynn A.} and Stricker, {Stephen A.} and Monroy, {Fernando P} and Wilson, {Wade D.} and Loker, {Eric S.}",
year = "1994",
month = "7",
doi = "10.1006/jipa.1994.1068",
language = "English (US)",
volume = "64",
pages = "52--61",
journal = "Journal of Invertebrate Pathology",
issn = "0022-2011",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Biomphalaria glabrata Hemolymph Lectins

T2 - Binding to Bacteria, Mammalian Erythrocytes, and to Sporocysts and Rediae of Echinostoma paraensei

AU - Hertel, Lynn A.

AU - Stricker, Stephen A.

AU - Monroy, Fernando P

AU - Wilson, Wade D.

AU - Loker, Eric S.

PY - 1994/7

Y1 - 1994/7

N2 - Polyclonal antibodies were raised in rabbits to two groups of diffusely staining M line Biomphalaria glabrata plasma polypeptides, of 150-210 and 70-120 kDa, designated as Group 1 molecules (G1M) and group 2 molecules (G2M), respectively. G1M and G2M are known to increase in abundance and to become more diverse following infection of B. glabrata with the digenetic trematode Echinostoma paraensei. These antibodies were used in conjunction with immunoblotting and slot blotting procedures to document binding of G1M/G2M from plasma of unexposed control snails (C plasma) or plasma from snails with 8-day infections of E. paraensei (I plasma) to various targets. Binding of G1M/G2M to both gram-positive and gram-negative bacteria, human A, B, and O and rabbit erythrocytes, and to sporocysts and rediae of E. paraensei was documented. Immunoblots revealed that erythrocytes are bound by a particular G2M band of 100-120 kDa present in I plasma that is in low concentration or lacking in C plasma. This explains previous results indicating that I plasma agglutinates all 4 erythrocytes examined whereas C plasma agglutinates only rabbit erythrocytes. More G1M/G2M binding to sporocysts occurred if I plasma rather than C plasma was used for incubations. Also, monosaccharide inhibition of G1M/G2M binding to sporocysts was observed in I plasma but not in C plasma. The results indicate that infection with E. paraensei induces production by B. glabrata of unique plasma polypeptides and that molecules present in I plasma can bind to the surfaces of non-self objects including E. paraensei larvae in a lectin-like fashion.

AB - Polyclonal antibodies were raised in rabbits to two groups of diffusely staining M line Biomphalaria glabrata plasma polypeptides, of 150-210 and 70-120 kDa, designated as Group 1 molecules (G1M) and group 2 molecules (G2M), respectively. G1M and G2M are known to increase in abundance and to become more diverse following infection of B. glabrata with the digenetic trematode Echinostoma paraensei. These antibodies were used in conjunction with immunoblotting and slot blotting procedures to document binding of G1M/G2M from plasma of unexposed control snails (C plasma) or plasma from snails with 8-day infections of E. paraensei (I plasma) to various targets. Binding of G1M/G2M to both gram-positive and gram-negative bacteria, human A, B, and O and rabbit erythrocytes, and to sporocysts and rediae of E. paraensei was documented. Immunoblots revealed that erythrocytes are bound by a particular G2M band of 100-120 kDa present in I plasma that is in low concentration or lacking in C plasma. This explains previous results indicating that I plasma agglutinates all 4 erythrocytes examined whereas C plasma agglutinates only rabbit erythrocytes. More G1M/G2M binding to sporocysts occurred if I plasma rather than C plasma was used for incubations. Also, monosaccharide inhibition of G1M/G2M binding to sporocysts was observed in I plasma but not in C plasma. The results indicate that infection with E. paraensei induces production by B. glabrata of unique plasma polypeptides and that molecules present in I plasma can bind to the surfaces of non-self objects including E. paraensei larvae in a lectin-like fashion.

KW - Biomphalaria glabrata

KW - comparative immunobiology

KW - Echinostoma paraensei

KW - Gastropoda

KW - lectins

KW - Mollusca

KW - non-self-recognition

KW - snail-trematode interactions

KW - Trematoda

UR - http://www.scopus.com/inward/record.url?scp=0028472344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028472344&partnerID=8YFLogxK

U2 - 10.1006/jipa.1994.1068

DO - 10.1006/jipa.1994.1068

M3 - Article

VL - 64

SP - 52

EP - 61

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

SN - 0022-2011

IS - 1

ER -