Best practices for analysing microbiomes

Rob Knight, Alison Vrbanac, Bryn C. Taylor, Alexander Aksenov, Chris Callewaert, Justine Debelius, Antonio Gonzalez, Tomasz Kosciolek, Laura Isobel McCall, Daniel McDonald, Alexey V. Melnik, James T. Morton, Jose Navas, Robert A. Quinn, Jon G. Sanders, Austin D. Swafford, Luke R. Thompson, Anupriya Tripathi, Zhenjiang Z. Xu, Jesse R. ZaneveldQiyun Zhu, James G Caporaso, Pieter C. Dorrestein

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Complex microbial communities shape the dynamics of various environments, ranging from the mammalian gastrointestinal tract to the soil. Advances in DNA sequencing technologies and data analysis have provided drastic improvements in microbiome analyses, for example, in taxonomic resolution, false discovery rate control and other properties, over earlier methods. In this Review, we discuss the best practices for performing a microbiome study, including experimental design, choice of molecular analysis technology, methods for data analysis and the integration of multiple omics data sets. We focus on recent findings that suggest that operational taxonomic unit-based analyses should be replaced with new methods that are based on exact sequence variants, methods for integrating metagenomic and metabolomic data, and issues surrounding compositional data analysis, where advances have been particularly rapid. We note that although some of these approaches are new, it is important to keep sight of the classic issues that arise during experimental design and relate to research reproducibility. We describe how keeping these issues in mind allows researchers to obtain more insight from their microbiome data sets.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalNature Reviews Microbiology
DOIs
StateAccepted/In press - May 23 2018

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Microbiota
Practice Guidelines
Research Design
Technology
Metagenomics
Metabolomics
DNA Sequence Analysis
Gastrointestinal Tract
Soil
Research Personnel
Research
Datasets

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Knight, R., Vrbanac, A., Taylor, B. C., Aksenov, A., Callewaert, C., Debelius, J., ... Dorrestein, P. C. (Accepted/In press). Best practices for analysing microbiomes. Nature Reviews Microbiology, 1-13. https://doi.org/10.1038/s41579-018-0029-9

Best practices for analysing microbiomes. / Knight, Rob; Vrbanac, Alison; Taylor, Bryn C.; Aksenov, Alexander; Callewaert, Chris; Debelius, Justine; Gonzalez, Antonio; Kosciolek, Tomasz; McCall, Laura Isobel; McDonald, Daniel; Melnik, Alexey V.; Morton, James T.; Navas, Jose; Quinn, Robert A.; Sanders, Jon G.; Swafford, Austin D.; Thompson, Luke R.; Tripathi, Anupriya; Xu, Zhenjiang Z.; Zaneveld, Jesse R.; Zhu, Qiyun; Caporaso, James G; Dorrestein, Pieter C.

In: Nature Reviews Microbiology, 23.05.2018, p. 1-13.

Research output: Contribution to journalArticle

Knight, R, Vrbanac, A, Taylor, BC, Aksenov, A, Callewaert, C, Debelius, J, Gonzalez, A, Kosciolek, T, McCall, LI, McDonald, D, Melnik, AV, Morton, JT, Navas, J, Quinn, RA, Sanders, JG, Swafford, AD, Thompson, LR, Tripathi, A, Xu, ZZ, Zaneveld, JR, Zhu, Q, Caporaso, JG & Dorrestein, PC 2018, 'Best practices for analysing microbiomes', Nature Reviews Microbiology, pp. 1-13. https://doi.org/10.1038/s41579-018-0029-9
Knight R, Vrbanac A, Taylor BC, Aksenov A, Callewaert C, Debelius J et al. Best practices for analysing microbiomes. Nature Reviews Microbiology. 2018 May 23;1-13. https://doi.org/10.1038/s41579-018-0029-9
Knight, Rob ; Vrbanac, Alison ; Taylor, Bryn C. ; Aksenov, Alexander ; Callewaert, Chris ; Debelius, Justine ; Gonzalez, Antonio ; Kosciolek, Tomasz ; McCall, Laura Isobel ; McDonald, Daniel ; Melnik, Alexey V. ; Morton, James T. ; Navas, Jose ; Quinn, Robert A. ; Sanders, Jon G. ; Swafford, Austin D. ; Thompson, Luke R. ; Tripathi, Anupriya ; Xu, Zhenjiang Z. ; Zaneveld, Jesse R. ; Zhu, Qiyun ; Caporaso, James G ; Dorrestein, Pieter C. / Best practices for analysing microbiomes. In: Nature Reviews Microbiology. 2018 ; pp. 1-13.
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