B chromosomes of maize (Zea mays L.) are positioned nonrandomly within sperm nuclei

Maxine Losoff Rusche, H. L. Mogensen, Annie Chaboud, Jean Emmanuel Faure, Mireille Rougier, Paul Keim, Christian Dumas

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We used fluorescence in situ hybridization to identify and map the position of B chromosomes (supernumerary chromosomes) within maize sperm cells. Observations on over 1,000 sperm cells from several genotypes show that, on average, the B chromosomes are positioned in the tip one-fourth of the sperm nucleus two-thirds of the time. In contrast, the centromeres and knobs of the A chromosomes (the normal set) are not restricted to the tip portion of the nucleus. To our knowledge, this is the first example of specific chromosome positioning within a plant gamete. Studies on nuclear architecture of somatic cells in both plants and animals suggest that chromosome behavior and gene expression may correlate with chromosome position within the nucleus. The functional significance of nonrandom positioning of the B chromosomes within maize sperm is as yet unclear.

Original languageEnglish (US)
Pages (from-to)231-234
Number of pages4
JournalSexual Plant Reproduction
Volume13
Issue number4
DOIs
StatePublished - 2000

Fingerprint

Chromosomes, Human, 4-5
Zea mays
Chromosome Positioning
Spermatozoa
Chromosomes
spermatozoa
chromosomes
corn
Plant Germ Cells
Centromere
centromeres
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
somatic cells
germ cells
Genotype
cells
Gene Expression
gene expression
B chromosomes

Keywords

  • B chromosomes
  • Fluorescence in situ hybridization
  • Maize sperm cells
  • Nuclear architecture

ASJC Scopus subject areas

  • Plant Science

Cite this

Rusche, M. L., Mogensen, H. L., Chaboud, A., Faure, J. E., Rougier, M., Keim, P., & Dumas, C. (2000). B chromosomes of maize (Zea mays L.) are positioned nonrandomly within sperm nuclei. Sexual Plant Reproduction, 13(4), 231-234. https://doi.org/10.1007/s004970000055

B chromosomes of maize (Zea mays L.) are positioned nonrandomly within sperm nuclei. / Rusche, Maxine Losoff; Mogensen, H. L.; Chaboud, Annie; Faure, Jean Emmanuel; Rougier, Mireille; Keim, Paul; Dumas, Christian.

In: Sexual Plant Reproduction, Vol. 13, No. 4, 2000, p. 231-234.

Research output: Contribution to journalArticle

Rusche, ML, Mogensen, HL, Chaboud, A, Faure, JE, Rougier, M, Keim, P & Dumas, C 2000, 'B chromosomes of maize (Zea mays L.) are positioned nonrandomly within sperm nuclei', Sexual Plant Reproduction, vol. 13, no. 4, pp. 231-234. https://doi.org/10.1007/s004970000055
Rusche, Maxine Losoff ; Mogensen, H. L. ; Chaboud, Annie ; Faure, Jean Emmanuel ; Rougier, Mireille ; Keim, Paul ; Dumas, Christian. / B chromosomes of maize (Zea mays L.) are positioned nonrandomly within sperm nuclei. In: Sexual Plant Reproduction. 2000 ; Vol. 13, No. 4. pp. 231-234.
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