B chromosome behavior in maize pollen as determined by a molecular probe

Losoff M. Rusche, H. Mogensen, Liang Shi, Paul S Keim, Mireille Rougier, Annie Chaboud, Christian Dumas

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The B chromosomes of maize typically undergo nondisjunction during the second microspore division (generative cell division). When the microspore nucleus contains only one B chromosome, two kinds of sperm result, one with two B chromosomes and one with no B chromosomes. The sperm with the B chromosomes preferentially fertilizes the egg cell. Previous studies of these phenomena have been limited to genetic analysis and chromosome spreads. In this study we show that a B chromosome-specific probe can be used with fluorescence in situ hybridization (FISH) analysis to detect the presence, location, and frequency of B chromosomes in intact interphase nuclei within mature pollen of maize. Using genetic line TB-10L18, our results indicate that nondisjunction of the B centromere occurs at an average frequency of 56.6%, based on four plants and 1306 pollen grains analyzed. This is consistent with the results of genetic studies using the same B-A translocation. In addition, our results suggest that B chromosome nondisjunction can occur during the first microspore division. Spatial distribution of the B chromosome-specific probe appears to be largely confined to one tip of the sperm nucleus, and a DNA fragment found outside the pollen nuclei often hybridizes to the B chromosome-specific probe.

Original languageEnglish (US)
Pages (from-to)1915-1921
Number of pages7
JournalGenetics
Volume147
Issue number4
StatePublished - Dec 1997

Fingerprint

Chromosomes, Human, 4-5
Molecular Probes
Pollen
Zea mays
Spermatozoa
Centromere
Interphase
Fluorescence In Situ Hybridization
Cell Division
Ovum
Chromosomes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Rusche, L. M., Mogensen, H., Shi, L., Keim, P. S., Rougier, M., Chaboud, A., & Dumas, C. (1997). B chromosome behavior in maize pollen as determined by a molecular probe. Genetics, 147(4), 1915-1921.

B chromosome behavior in maize pollen as determined by a molecular probe. / Rusche, Losoff M.; Mogensen, H.; Shi, Liang; Keim, Paul S; Rougier, Mireille; Chaboud, Annie; Dumas, Christian.

In: Genetics, Vol. 147, No. 4, 12.1997, p. 1915-1921.

Research output: Contribution to journalArticle

Rusche, LM, Mogensen, H, Shi, L, Keim, PS, Rougier, M, Chaboud, A & Dumas, C 1997, 'B chromosome behavior in maize pollen as determined by a molecular probe', Genetics, vol. 147, no. 4, pp. 1915-1921.
Rusche LM, Mogensen H, Shi L, Keim PS, Rougier M, Chaboud A et al. B chromosome behavior in maize pollen as determined by a molecular probe. Genetics. 1997 Dec;147(4):1915-1921.
Rusche, Losoff M. ; Mogensen, H. ; Shi, Liang ; Keim, Paul S ; Rougier, Mireille ; Chaboud, Annie ; Dumas, Christian. / B chromosome behavior in maize pollen as determined by a molecular probe. In: Genetics. 1997 ; Vol. 147, No. 4. pp. 1915-1921.
@article{748059c10cdb48bea1596444ceb04130,
title = "B chromosome behavior in maize pollen as determined by a molecular probe",
abstract = "The B chromosomes of maize typically undergo nondisjunction during the second microspore division (generative cell division). When the microspore nucleus contains only one B chromosome, two kinds of sperm result, one with two B chromosomes and one with no B chromosomes. The sperm with the B chromosomes preferentially fertilizes the egg cell. Previous studies of these phenomena have been limited to genetic analysis and chromosome spreads. In this study we show that a B chromosome-specific probe can be used with fluorescence in situ hybridization (FISH) analysis to detect the presence, location, and frequency of B chromosomes in intact interphase nuclei within mature pollen of maize. Using genetic line TB-10L18, our results indicate that nondisjunction of the B centromere occurs at an average frequency of 56.6{\%}, based on four plants and 1306 pollen grains analyzed. This is consistent with the results of genetic studies using the same B-A translocation. In addition, our results suggest that B chromosome nondisjunction can occur during the first microspore division. Spatial distribution of the B chromosome-specific probe appears to be largely confined to one tip of the sperm nucleus, and a DNA fragment found outside the pollen nuclei often hybridizes to the B chromosome-specific probe.",
author = "Rusche, {Losoff M.} and H. Mogensen and Liang Shi and Keim, {Paul S} and Mireille Rougier and Annie Chaboud and Christian Dumas",
year = "1997",
month = "12",
language = "English (US)",
volume = "147",
pages = "1915--1921",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "4",

}

TY - JOUR

T1 - B chromosome behavior in maize pollen as determined by a molecular probe

AU - Rusche, Losoff M.

AU - Mogensen, H.

AU - Shi, Liang

AU - Keim, Paul S

AU - Rougier, Mireille

AU - Chaboud, Annie

AU - Dumas, Christian

PY - 1997/12

Y1 - 1997/12

N2 - The B chromosomes of maize typically undergo nondisjunction during the second microspore division (generative cell division). When the microspore nucleus contains only one B chromosome, two kinds of sperm result, one with two B chromosomes and one with no B chromosomes. The sperm with the B chromosomes preferentially fertilizes the egg cell. Previous studies of these phenomena have been limited to genetic analysis and chromosome spreads. In this study we show that a B chromosome-specific probe can be used with fluorescence in situ hybridization (FISH) analysis to detect the presence, location, and frequency of B chromosomes in intact interphase nuclei within mature pollen of maize. Using genetic line TB-10L18, our results indicate that nondisjunction of the B centromere occurs at an average frequency of 56.6%, based on four plants and 1306 pollen grains analyzed. This is consistent with the results of genetic studies using the same B-A translocation. In addition, our results suggest that B chromosome nondisjunction can occur during the first microspore division. Spatial distribution of the B chromosome-specific probe appears to be largely confined to one tip of the sperm nucleus, and a DNA fragment found outside the pollen nuclei often hybridizes to the B chromosome-specific probe.

AB - The B chromosomes of maize typically undergo nondisjunction during the second microspore division (generative cell division). When the microspore nucleus contains only one B chromosome, two kinds of sperm result, one with two B chromosomes and one with no B chromosomes. The sperm with the B chromosomes preferentially fertilizes the egg cell. Previous studies of these phenomena have been limited to genetic analysis and chromosome spreads. In this study we show that a B chromosome-specific probe can be used with fluorescence in situ hybridization (FISH) analysis to detect the presence, location, and frequency of B chromosomes in intact interphase nuclei within mature pollen of maize. Using genetic line TB-10L18, our results indicate that nondisjunction of the B centromere occurs at an average frequency of 56.6%, based on four plants and 1306 pollen grains analyzed. This is consistent with the results of genetic studies using the same B-A translocation. In addition, our results suggest that B chromosome nondisjunction can occur during the first microspore division. Spatial distribution of the B chromosome-specific probe appears to be largely confined to one tip of the sperm nucleus, and a DNA fragment found outside the pollen nuclei often hybridizes to the B chromosome-specific probe.

UR - http://www.scopus.com/inward/record.url?scp=0030784727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030784727&partnerID=8YFLogxK

M3 - Article

C2 - 9409846

AN - SCOPUS:0030784727

VL - 147

SP - 1915

EP - 1921

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 4

ER -