Allozyme variation in interior Douglas-fir

Association with growth and resistance to western spruce budworm herbivory

Z. Chen, Thomas E Kolb, K. M. Clancy, V. D. Hipkins, L. E. DeWald

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We used starch gel electrophoresis to investigate levels of genetic variation between trees of interior Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco var. glauca) that were phenotypically resistant versus susceptible to defoliation by the western spruce budworm (Choristoneura occidentalis Freeman). We also investigated the association between allozyme variation and tree growth traits. Overall, the phenotypically resistant trees had a lower allelic heterozygosity (p = 0.020) compared with susceptible trees. However, this difference between resistant and susceptible trees primarily occurred at the Buena Vista, Colorado, site rather than the Deckers, Colorado, and Jacob Lake, Arizona, sites. Among 25 loci we examined, the resistant trees also had a higher frequency of the most common alleles (p = 0.057) and a higher proportion of homozygous genotypes, especially at loci FEST-1 (p = 0.004), ACO-1 (p = 0.080), and 6PGD-1 (p = 0.084). The higher allelic heterozygosity in susceptible trees was mainly due to their higher proportion of uncommon and (or) rare alleles. Compared with susceptible trees, resistant trees had higher mean radial growth rates (p = 0.047) and less temporal variability in growth rate over 25 years (p = 0.037). Mean radial growth rate and average tree heterozygosity were not related at any site (p = 0.316). Relationships between temporal variability in growth rate and tree heterozygosity were inconsistent among sites. Our results suggest that phenotypic differences in resistance of interior Douglas-fir to western spruce budworm defoliation are partly caused by genetic differences among trees.

Original languageEnglish (US)
Pages (from-to)1691-1700
Number of pages10
JournalCanadian Journal of Forest Research
Volume31
Issue number10
DOIs
StatePublished - 2001

Fingerprint

Choristoneura occidentalis
allozyme
Pseudotsuga menziesii
herbivory
allozymes
herbivores
heterozygosity
defoliation
allele
alleles
loci
starch gels
growth traits
homozygosity
tree growth
gel electrophoresis
starch

ASJC Scopus subject areas

  • Forestry
  • Plant Science

Cite this

Allozyme variation in interior Douglas-fir : Association with growth and resistance to western spruce budworm herbivory. / Chen, Z.; Kolb, Thomas E; Clancy, K. M.; Hipkins, V. D.; DeWald, L. E.

In: Canadian Journal of Forest Research, Vol. 31, No. 10, 2001, p. 1691-1700.

Research output: Contribution to journalArticle

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