African lineage Brucella melitensis Isolates from Omani livestock

Jeffrey T Foster, Faith M. Walker, Brandy D. Rannals, M. Hammad Hussain, Kevin P. Drees, Rebekah V. Tiller, Alex R. Hoffmaster, Abdulmajeed Al-Rawahi, Paul S Keim, Muhammad Saqib

Research output: Contribution to journalArticle

1 Citations (Scopus)

Abstract

Brucellosis is a common livestock disease in the Middle East and North Africa, but remains poorly described in the region both genetically and epidemiologically. Traditionally found in goats and sheep, Brucella melitensis is increasingly recognized as infecting camels. Most studies of brucellosis in camels to date have focused on serological surveys, providing only limited understanding of the molecular epidemiology of circulating strains. We genotyped B. melitensis isolates from Omani camels using whole genome SNP assays and VNTRs to provide context for regional brucellosis cases. We identified a lineage of B. melitensis circulating in camels as well as in goats, sheep, and cattle in Oman. This lineage is genetically distinct from most genotypes from the Arabian Peninsula and from isolates from much of the rest of the Middle East. We then developed diagnostic assays that rapidly identify strains from this lineage. In analyses of genotypes from throughout the region, Omani isolates were genetically most closely related to strains from brucellosis cases in humans and livestock in North Africa. Our findings suggest an African origin for B. melitensis in Oman that has likely occurred through the trade of infected livestock. Moreover, African lineages of B. melitensis appear to be undersampled and consequently are underrepresented in genetic databases for Brucella. As we begin to more fully understand global genomic diversity of B. melitensis, finding and characterizing these unique but widespread lineages is essential. We predict that increased sampling of humans and livestock in Africa will reveal little known diversity in this important zoonotic pathogen.

Original languageEnglish (US)
Article number2702
JournalFrontiers in Microbiology
Volume8
Issue numberJAN
DOIs
StatePublished - Jan 15 2018

Fingerprint

Brucella melitensis
Livestock
Camelus
Brucellosis
Oman
Northern Africa
Middle East
Goats
Sheep
Genotype
Genetic Databases
Eastern Africa
Brucella
Molecular Epidemiology
Zoonoses
Single Nucleotide Polymorphism
Genome

Keywords

  • Brucella melitensis
  • Brucellosis
  • Camels
  • MLVA
  • Oman
  • SNP genotyping

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Foster, J. T., Walker, F. M., Rannals, B. D., Hammad Hussain, M., Drees, K. P., Tiller, R. V., ... Saqib, M. (2018). African lineage Brucella melitensis Isolates from Omani livestock. Frontiers in Microbiology, 8(JAN), [2702]. https://doi.org/10.3389/fmicb.2017.02702

African lineage Brucella melitensis Isolates from Omani livestock. / Foster, Jeffrey T; Walker, Faith M.; Rannals, Brandy D.; Hammad Hussain, M.; Drees, Kevin P.; Tiller, Rebekah V.; Hoffmaster, Alex R.; Al-Rawahi, Abdulmajeed; Keim, Paul S; Saqib, Muhammad.

In: Frontiers in Microbiology, Vol. 8, No. JAN, 2702, 15.01.2018.

Research output: Contribution to journalArticle

Foster, JT, Walker, FM, Rannals, BD, Hammad Hussain, M, Drees, KP, Tiller, RV, Hoffmaster, AR, Al-Rawahi, A, Keim, PS & Saqib, M 2018, 'African lineage Brucella melitensis Isolates from Omani livestock' Frontiers in Microbiology, vol. 8, no. JAN, 2702. https://doi.org/10.3389/fmicb.2017.02702
Foster JT, Walker FM, Rannals BD, Hammad Hussain M, Drees KP, Tiller RV et al. African lineage Brucella melitensis Isolates from Omani livestock. Frontiers in Microbiology. 2018 Jan 15;8(JAN). 2702. https://doi.org/10.3389/fmicb.2017.02702
Foster, Jeffrey T ; Walker, Faith M. ; Rannals, Brandy D. ; Hammad Hussain, M. ; Drees, Kevin P. ; Tiller, Rebekah V. ; Hoffmaster, Alex R. ; Al-Rawahi, Abdulmajeed ; Keim, Paul S ; Saqib, Muhammad. / African lineage Brucella melitensis Isolates from Omani livestock. In: Frontiers in Microbiology. 2018 ; Vol. 8, No. JAN.
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