Activity-dependent labeling of oxygenase enzymes in a trichloroethene-contaminated groundwater site

M. Hope Lee, Scott C. Clingenpeel, Owen P. Leiser, Ryan A. Wymore, Kent S. Sorenson, Maribeth E Watwood

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A variety of naturally occurring bacteria produce enzymes that cometabolically degrade trichloroethene (TCE), including organisms with aerobic oxygenases. Groundwater contaminated with TCE was collected from the aerobic region of the Test Area North site of the Idaho National Laboratory. Samples were evaluated with enzyme activity probes, and resulted in measurable detection of toluene oxygenase activity (6-79% of the total microbial cells). Wells from both inside and outside contaminated plume showed activity. Toluene oxygenase-specific PCR primers determined that toluene-degrading genes were present in all groundwater samples evaluated. In addition, bacterial isolates were obtained and possessed toluene oxygenase enzymes, demonstrated activity, and were dominated by the phylotype Pseudomonas. This study demonstrated, through the use of enzymatic probes and oxygenase gene identification, that indigenous microorganisms at a contaminated site were cometabolically active. Documentation such as this can be used to substantiate observations of natural attenuation of TCE-contaminated groundwater plumes.

Original languageEnglish (US)
Pages (from-to)238-246
Number of pages9
JournalEnvironmental Pollution
Volume153
Issue number1
DOIs
StatePublished - May 2008

Fingerprint

Trichloroethylene
Oxygenases
Groundwater
trichloroethylene
toluene
Labeling
Toluene
Enzymes
enzyme
groundwater
enzyme activity
Enzyme activity
plume
probe
Genes
natural attenuation
gene
Natural attenuation
Pseudomonas
microorganism

Keywords

  • Activity
  • Cometabolism
  • Groundwater
  • Natural attenuation
  • Trichloroethene (TCE)

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Science(all)
  • Pollution

Cite this

Activity-dependent labeling of oxygenase enzymes in a trichloroethene-contaminated groundwater site. / Lee, M. Hope; Clingenpeel, Scott C.; Leiser, Owen P.; Wymore, Ryan A.; Sorenson, Kent S.; Watwood, Maribeth E.

In: Environmental Pollution, Vol. 153, No. 1, 05.2008, p. 238-246.

Research output: Contribution to journalArticle

Lee, M. Hope ; Clingenpeel, Scott C. ; Leiser, Owen P. ; Wymore, Ryan A. ; Sorenson, Kent S. ; Watwood, Maribeth E. / Activity-dependent labeling of oxygenase enzymes in a trichloroethene-contaminated groundwater site. In: Environmental Pollution. 2008 ; Vol. 153, No. 1. pp. 238-246.
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