Actin mutations that show suppression with fimbrin mutations identify a likely fimbrin-binding site on actin

Jerry E. Honts, Tanya S. Sandrock, Sharon M. Brower, Johanna L. O'Dell, Alison Adams

Research output: Contribution to journalArticle

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Abstract

Actin interacts with a large number of different proteins that modulate its assembly and mediate its functions. One such protein is the yeast actin- binding protein Sac6p, which is homologous to vertebrate fimbrin (Adams, A. E. M., D. Botstein, and D. G. Drubin. 1991. Nature (Lond.). 354:404-408.). Sac6p was originally identified both genetically (Adams, A. E. M., and D. Botstein. 1989. Genetics. 121:675-683.) by dominant, reciprocal suppression of a temperature-sensitive yeast actin mutation (act1-1), as well as biochemically (Drubin, D. G., K. G. Miller, and D. Botstein. 1988. J. Cell Biol. 107:2551-2561.). To identify the region on actin that interacts with Sac6p, we have analyzed eight different act1 mutations that show suppression with sac6 mutant alleles, and have asked whether (a) these mutations occur in a small defined region on the crystal structure of actin; and (b) the mutant actins are defective in their interaction with Sac6p in vitro. Sequence analysis indicates that all of these mutations change residues that cluster in the small domain of the actin crystal structure, suggesting that this region is an important part of the Sac6p-binding domain. Biochemical analysis reveals defects in the ability of several of the mutant actins to bind Sac6p, and a reduction in Sac6p-induced cross-linking of mutant actin filaments. Together, these observations identify a likely site of interaction of fimbrin on actin.

Original languageEnglish (US)
Pages (from-to)413-422
Number of pages10
JournalJournal of Cell Biology
Volume126
Issue number2
DOIs
StatePublished - Jul 1994
Externally publishedYes

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Actins
Binding Sites
Mutation
Microfilament Proteins
Fungal Proteins
plastin
Actin Cytoskeleton
Sequence Analysis
Vertebrates
Proteins
Yeasts
Alleles
Temperature

ASJC Scopus subject areas

  • Cell Biology

Cite this

Actin mutations that show suppression with fimbrin mutations identify a likely fimbrin-binding site on actin. / Honts, Jerry E.; Sandrock, Tanya S.; Brower, Sharon M.; O'Dell, Johanna L.; Adams, Alison.

In: Journal of Cell Biology, Vol. 126, No. 2, 07.1994, p. 413-422.

Research output: Contribution to journalArticle

Honts, Jerry E. ; Sandrock, Tanya S. ; Brower, Sharon M. ; O'Dell, Johanna L. ; Adams, Alison. / Actin mutations that show suppression with fimbrin mutations identify a likely fimbrin-binding site on actin. In: Journal of Cell Biology. 1994 ; Vol. 126, No. 2. pp. 413-422.
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