Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR

Erin P. Price, Derek S. Sarovich, Jessica R. Webb, Jennifer L. Ginther, Mark Mayo, James M. Cook, Meagan L. Seymour, Mirjam Kaestli, Vanessa Theobald, Carina M. Hall, Joseph D. Busch, Jeffrey T Foster, Paul S Keim, David M Wagner, Apichai Tuanyok, Talima R Pearson, Bart J. Currie

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown's medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown's agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown's-positive colonies that are not B. pseudomallei.

Original languageEnglish (US)
Article numbere71647
JournalPLoS One
Volume8
Issue number8
DOIs
StatePublished - Aug 13 2013

Fingerprint

Burkholderia ubonensis
Burkholderia pseudomallei
Burkholderia
Real-Time Polymerase Chain Reaction
Assays
Bacteria
quantitative polymerase chain reaction
Biocontrol
Agar
Soils
Burkholderia cepacia complex
Melioidosis
bacteria
organisms
assays
Soil
biological control agents
niches

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Price, E. P., Sarovich, D. S., Webb, J. R., Ginther, J. L., Mayo, M., Cook, J. M., ... Currie, B. J. (2013). Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR. PLoS One, 8(8), [e71647]. https://doi.org/10.1371/journal.pone.0071647

Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR. / Price, Erin P.; Sarovich, Derek S.; Webb, Jessica R.; Ginther, Jennifer L.; Mayo, Mark; Cook, James M.; Seymour, Meagan L.; Kaestli, Mirjam; Theobald, Vanessa; Hall, Carina M.; Busch, Joseph D.; Foster, Jeffrey T; Keim, Paul S; Wagner, David M; Tuanyok, Apichai; Pearson, Talima R; Currie, Bart J.

In: PLoS One, Vol. 8, No. 8, e71647, 13.08.2013.

Research output: Contribution to journalArticle

Price, EP, Sarovich, DS, Webb, JR, Ginther, JL, Mayo, M, Cook, JM, Seymour, ML, Kaestli, M, Theobald, V, Hall, CM, Busch, JD, Foster, JT, Keim, PS, Wagner, DM, Tuanyok, A, Pearson, TR & Currie, BJ 2013, 'Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR', PLoS One, vol. 8, no. 8, e71647. https://doi.org/10.1371/journal.pone.0071647
Price, Erin P. ; Sarovich, Derek S. ; Webb, Jessica R. ; Ginther, Jennifer L. ; Mayo, Mark ; Cook, James M. ; Seymour, Meagan L. ; Kaestli, Mirjam ; Theobald, Vanessa ; Hall, Carina M. ; Busch, Joseph D. ; Foster, Jeffrey T ; Keim, Paul S ; Wagner, David M ; Tuanyok, Apichai ; Pearson, Talima R ; Currie, Bart J. / Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR. In: PLoS One. 2013 ; Vol. 8, No. 8.
@article{a402beb5cc4447d79cfe9a7926218420,
title = "Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR",
abstract = "Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95{\%} of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown's medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown's agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown's-positive colonies that are not B. pseudomallei.",
author = "Price, {Erin P.} and Sarovich, {Derek S.} and Webb, {Jessica R.} and Ginther, {Jennifer L.} and Mark Mayo and Cook, {James M.} and Seymour, {Meagan L.} and Mirjam Kaestli and Vanessa Theobald and Hall, {Carina M.} and Busch, {Joseph D.} and Foster, {Jeffrey T} and Keim, {Paul S} and Wagner, {David M} and Apichai Tuanyok and Pearson, {Talima R} and Currie, {Bart J.}",
year = "2013",
month = "8",
day = "13",
doi = "10.1371/journal.pone.0071647",
language = "English (US)",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR

AU - Price, Erin P.

AU - Sarovich, Derek S.

AU - Webb, Jessica R.

AU - Ginther, Jennifer L.

AU - Mayo, Mark

AU - Cook, James M.

AU - Seymour, Meagan L.

AU - Kaestli, Mirjam

AU - Theobald, Vanessa

AU - Hall, Carina M.

AU - Busch, Joseph D.

AU - Foster, Jeffrey T

AU - Keim, Paul S

AU - Wagner, David M

AU - Tuanyok, Apichai

AU - Pearson, Talima R

AU - Currie, Bart J.

PY - 2013/8/13

Y1 - 2013/8/13

N2 - Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown's medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown's agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown's-positive colonies that are not B. pseudomallei.

AB - Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown's medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown's agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown's-positive colonies that are not B. pseudomallei.

UR - http://www.scopus.com/inward/record.url?scp=84881490139&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881490139&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0071647

DO - 10.1371/journal.pone.0071647

M3 - Article

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e71647

ER -