A suppressor of a mating-type limited zygotic lethal allele also suppresses uniparental chloroplast gene transmission in Chlamydomonas monoica

Karen P VanWinkle-Swift, R. Hoffman, L. Shi, S. Parker

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Uniparental inheritance of Chlamydomonas chloroplast genes is thought to involve modification of maternal (mt+) chloroplast genomes to protect against a nuclease that is activated after gamete fusion. The mating-type limited mtl-1 mutant strain of Chlamydomonas monoica is unable to protect mt+-derived chloroplast DNA. Zygotes homozygous for mtl-1 lose all chloroplast DNA and fail to germinate. We have selected for suppression of this zygote-specific lethality, and have obtained 20 mutant strains that produce viable homozygotes despite the continued presence of the mtl-1 allele. Genetic analysis indicates that the suppressor mutations are all recessive alleles at a single locus (sup-1) which is unlinked to mtl-1. Crosses between sup-1 strains carrying distinctive chloroplast antibiotic resistance markers also show predominantly biparental chloroplast gene transmission. Chloroplast nucleoids of both parental origins (stained with the DNA-specific fluorochrome, DAPI) are retained in the zygotes homozygous for sup-1. The data are compatible with the idea that the sup-1 (suppressor of uniparental inheritance) locus may encode a chloroplast DNA nuclease that is expressed from both parental genomes.

Original languageEnglish (US)
Pages (from-to)867-877
Number of pages11
JournalGenetics
Volume136
Issue number3
StatePublished - 1994

Fingerprint

Lethal Genes
Chloroplast Genes
Chloroplast DNA
Chlamydomonas
Zygote
Chloroplasts
Alleles
Chloroplast Genome
Genetic Suppression
Deoxyribonucleases
Homozygote
Microbial Drug Resistance
Fluorescent Dyes
Germ Cells
Mothers
Genome
DNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

A suppressor of a mating-type limited zygotic lethal allele also suppresses uniparental chloroplast gene transmission in Chlamydomonas monoica. / VanWinkle-Swift, Karen P; Hoffman, R.; Shi, L.; Parker, S.

In: Genetics, Vol. 136, No. 3, 1994, p. 867-877.

Research output: Contribution to journalArticle

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