A rapid method for quantifying heavy atom derivatives for multiple isomorphous replacement in protein crystallography

Leslie A. Colip, Andrew T Koppisch, Richard D. Broene, Jennifer A. Berger, Sharon M. Baldwin, Michael N. Harris, Lori J. Peterson, Benjamin P. Warner, Eva R. Birnbaum

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A rapid and simple X-ray fluorescence-based method is reported for characterizing heavy atom derivatives of proteins for protein crystallography using multiple isomorphous replacement (MIR). MIR is a widely used technique for solving protein crystallographic structures which requires that a 'heavy atom' be incorporated into the protein to provide a strong signal in the diffraction pattern. Current methods for determining the effectiveness of these protein-heavy atom reactions are not always successful. In contrast, X-ray fluorescence quickly determines the presence of heavy atom modifications of proteins and the stoichiometry of these modifications.

Original languageEnglish (US)
Pages (from-to)329-332
Number of pages4
JournalJournal of Applied Crystallography
Volume42
Issue number2
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Crystallography
Derivatives
Atoms
Proteins
Fluorescence
X-Rays
X rays
Stoichiometry
Diffraction patterns

Keywords

  • Heavy atom derivatization
  • Multiple isomorphous replacement
  • Protein crystallography
  • X-ray fluorescence

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A rapid method for quantifying heavy atom derivatives for multiple isomorphous replacement in protein crystallography. / Colip, Leslie A.; Koppisch, Andrew T; Broene, Richard D.; Berger, Jennifer A.; Baldwin, Sharon M.; Harris, Michael N.; Peterson, Lori J.; Warner, Benjamin P.; Birnbaum, Eva R.

In: Journal of Applied Crystallography, Vol. 42, No. 2, 2009, p. 329-332.

Research output: Contribution to journalArticle

Colip, LA, Koppisch, AT, Broene, RD, Berger, JA, Baldwin, SM, Harris, MN, Peterson, LJ, Warner, BP & Birnbaum, ER 2009, 'A rapid method for quantifying heavy atom derivatives for multiple isomorphous replacement in protein crystallography', Journal of Applied Crystallography, vol. 42, no. 2, pp. 329-332. https://doi.org/10.1107/S0021889809000077
Colip, Leslie A. ; Koppisch, Andrew T ; Broene, Richard D. ; Berger, Jennifer A. ; Baldwin, Sharon M. ; Harris, Michael N. ; Peterson, Lori J. ; Warner, Benjamin P. ; Birnbaum, Eva R. / A rapid method for quantifying heavy atom derivatives for multiple isomorphous replacement in protein crystallography. In: Journal of Applied Crystallography. 2009 ; Vol. 42, No. 2. pp. 329-332.
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AU - Harris, Michael N.

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AU - Warner, Benjamin P.

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