A rapid DNA extraction method for sugarcane and its relatives

Rhonda J. Honeycutt, Bruno W S Sobral, Paul S Keim, James E. Irvine

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A simple DNA extraction method based on CTAB precipitation was used to obtain DNA from members of the genus Saccharum and related species. DNA yields and purities were similar for all Saccharum species sampled. The method described here resulted in high quality total DNA suitable for polymerase chain reaction (PCR)-based techniques as well as restriction endonuclease digestion, Southern hybridization, and DNA cycle-sequencing.

Original languageEnglish (US)
Pages (from-to)66-72
Number of pages7
JournalPlant Molecular Biology Reporter
Volume10
Issue number1
DOIs
StatePublished - Feb 1992

Fingerprint

Saccharum
sugarcane
DNA
DNA Restriction Enzymes
DNA-Directed DNA Polymerase
DNA Sequence Analysis
Digestion
Polymerase chain reaction
restriction endonucleases
DNA-directed DNA polymerase
methodology
purity
Southern blotting
Polymerase Chain Reaction
digestion
polymerase chain reaction

Keywords

  • Andropogoneae
  • DNA extraction
  • grasses
  • Saccharum complex
  • sugarcane

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Plant Science

Cite this

A rapid DNA extraction method for sugarcane and its relatives. / Honeycutt, Rhonda J.; Sobral, Bruno W S; Keim, Paul S; Irvine, James E.

In: Plant Molecular Biology Reporter, Vol. 10, No. 1, 02.1992, p. 66-72.

Research output: Contribution to journalArticle

Honeycutt, Rhonda J. ; Sobral, Bruno W S ; Keim, Paul S ; Irvine, James E. / A rapid DNA extraction method for sugarcane and its relatives. In: Plant Molecular Biology Reporter. 1992 ; Vol. 10, No. 1. pp. 66-72.
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