A rapid DNA extraction method for sugarcane and its relatives

Rhonda J. Honeycutt, Bruno W.S. Sobral, Paul Keim, James E. Irvine

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A simple DNA extraction method based on CTAB precipitation was used to obtain DNA from members of the genus Saccharum and related species. DNA yields and purities were similar for all Saccharum species sampled. The method described here resulted in high quality total DNA suitable for polymerase chain reaction (PCR)-based techniques as well as restriction endonuclease digestion, Southern hybridization, and DNA cycle-sequencing.

Original languageEnglish (US)
Pages (from-to)66-72
Number of pages7
JournalPlant Molecular Biology Reporter
Volume10
Issue number1
DOIs
StatePublished - Feb 1 1992

Keywords

  • Andropogoneae
  • DNA extraction
  • Saccharum complex
  • grasses
  • sugarcane

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

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