A horizontal gene transfer event defines two distinct groups within Burkholderia pseudomallei that have dissimilar geographic distributions

Apichai Tuanyok, Raymond K. Auerbach, Thomas S. Brettin, David C. Bruce, A. Christine Munk, J. Chris Detter, Talima R Pearson, Heidie Hornstra, Rasana W. Sermswan, Vanaporn Wuthiekanun, Sharon J. Peacock, Bart J. Currie, Paul S Keim, David M Wagner

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Burkholderia pseudomallei is the etioiogic agent of melioidosis. Many disease manifestations are associated with melioidosis, and the mechanisms causing this variation are unknown; genomic differences among strains offer one explanation. We compared the genome sequences of two strains of B. pseudomallei: the original reference strain K96243 from Thailand and strain MSHR305 from Australia. We identified a variable homologous region between the two strains. This region was previously identified in comparisons of the genome of B. pseudomallei strain K96243 with the genome of strain E264 from the closely related B. thailandensis. In that comparison, K96243 was shown to possess a horizontally acquired rersinia-like fimbrial (YLF) gene cluster. Here, we show that the homologous genomic region in B. pseudomallei strain 305 is similar to that previously identified in B. thailandensis strain E264. We have named this region in B. pseudomallei strain 305 the B. thailandensis-like flagellum and chemotaxis (BTFC) gene cluster. We screened for these different genomic components across additional genome sequences and 571 B. pseudomallei DNA extracts obtained from regions of endemicity. These alternate genomic states define two distinct groups within B. pseudomallei: all strains contained either the BTFC gene cluster (group BTFC) or the YLF gene cluster (group YLF). These two groups have distinct geographic distributions: group BTFC is dominant in Australia, and group YLF is dominant in Thailand and elsewhere. In addition, clinical isolates are more likely to belong to group YLF, whereas environmental isolates are more likely to belong to group BTFC. These groups should be further characterized in an animal model.

Original languageEnglish (US)
Pages (from-to)9044-9049
Number of pages6
JournalJournal of Bacteriology
Volume189
Issue number24
DOIs
StatePublished - Dec 2007

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Burkholderia pseudomallei
Horizontal Gene Transfer
Flagella
Chemotaxis
Multigene Family
Genome
Melioidosis
Thailand
Animal Models
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

A horizontal gene transfer event defines two distinct groups within Burkholderia pseudomallei that have dissimilar geographic distributions. / Tuanyok, Apichai; Auerbach, Raymond K.; Brettin, Thomas S.; Bruce, David C.; Munk, A. Christine; Detter, J. Chris; Pearson, Talima R; Hornstra, Heidie; Sermswan, Rasana W.; Wuthiekanun, Vanaporn; Peacock, Sharon J.; Currie, Bart J.; Keim, Paul S; Wagner, David M.

In: Journal of Bacteriology, Vol. 189, No. 24, 12.2007, p. 9044-9049.

Research output: Contribution to journalArticle

Tuanyok, A, Auerbach, RK, Brettin, TS, Bruce, DC, Munk, AC, Detter, JC, Pearson, TR, Hornstra, H, Sermswan, RW, Wuthiekanun, V, Peacock, SJ, Currie, BJ, Keim, PS & Wagner, DM 2007, 'A horizontal gene transfer event defines two distinct groups within Burkholderia pseudomallei that have dissimilar geographic distributions', Journal of Bacteriology, vol. 189, no. 24, pp. 9044-9049. https://doi.org/10.1128/JB.01264-07
Tuanyok, Apichai ; Auerbach, Raymond K. ; Brettin, Thomas S. ; Bruce, David C. ; Munk, A. Christine ; Detter, J. Chris ; Pearson, Talima R ; Hornstra, Heidie ; Sermswan, Rasana W. ; Wuthiekanun, Vanaporn ; Peacock, Sharon J. ; Currie, Bart J. ; Keim, Paul S ; Wagner, David M. / A horizontal gene transfer event defines two distinct groups within Burkholderia pseudomallei that have dissimilar geographic distributions. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 24. pp. 9044-9049.
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abstract = "Burkholderia pseudomallei is the etioiogic agent of melioidosis. Many disease manifestations are associated with melioidosis, and the mechanisms causing this variation are unknown; genomic differences among strains offer one explanation. We compared the genome sequences of two strains of B. pseudomallei: the original reference strain K96243 from Thailand and strain MSHR305 from Australia. We identified a variable homologous region between the two strains. This region was previously identified in comparisons of the genome of B. pseudomallei strain K96243 with the genome of strain E264 from the closely related B. thailandensis. In that comparison, K96243 was shown to possess a horizontally acquired rersinia-like fimbrial (YLF) gene cluster. Here, we show that the homologous genomic region in B. pseudomallei strain 305 is similar to that previously identified in B. thailandensis strain E264. We have named this region in B. pseudomallei strain 305 the B. thailandensis-like flagellum and chemotaxis (BTFC) gene cluster. We screened for these different genomic components across additional genome sequences and 571 B. pseudomallei DNA extracts obtained from regions of endemicity. These alternate genomic states define two distinct groups within B. pseudomallei: all strains contained either the BTFC gene cluster (group BTFC) or the YLF gene cluster (group YLF). These two groups have distinct geographic distributions: group BTFC is dominant in Australia, and group YLF is dominant in Thailand and elsewhere. In addition, clinical isolates are more likely to belong to group YLF, whereas environmental isolates are more likely to belong to group BTFC. These groups should be further characterized in an animal model.",
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AU - Hornstra, Heidie

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AU - Wuthiekanun, Vanaporn

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