A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

L. J. Kenefic, J. Beaudry, C. Trim, L. Huynh, S. Zanecki, M. Matthews, J. Schupp, M. Van Ert, P. Keim

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

Original languageEnglish (US)
Pages (from-to)269-272
Number of pages4
JournalJournal of Microbiological Methods
Volume73
Issue number3
DOIs
StatePublished - Jun 1 2008

    Fingerprint

Keywords

  • Bacillus anthracis
  • Capillary electrophoresis
  • MLVA
  • SNR

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this