A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

L. J. Kenefic, J. Beaudry, C. Trim, L. Huynh, S. Zanecki, M. Matthews, J. Schupp, M. Van Ert, Paul S Keim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

Original languageEnglish (US)
Pages (from-to)269-272
Number of pages4
JournalJournal of Microbiological Methods
Volume73
Issue number3
DOIs
StatePublished - Jun 2008

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Bacillus anthracis
Nucleotides
Nucleic Acid Repetitive Sequences
Multiplex Polymerase Chain Reaction
Capillary Electrophoresis
DNA Sequence Analysis

Keywords

  • Bacillus anthracis
  • Capillary electrophoresis
  • MLVA
  • SNR

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis. / Kenefic, L. J.; Beaudry, J.; Trim, C.; Huynh, L.; Zanecki, S.; Matthews, M.; Schupp, J.; Van Ert, M.; Keim, Paul S.

In: Journal of Microbiological Methods, Vol. 73, No. 3, 06.2008, p. 269-272.

Research output: Contribution to journalArticle

Kenefic, LJ, Beaudry, J, Trim, C, Huynh, L, Zanecki, S, Matthews, M, Schupp, J, Van Ert, M & Keim, PS 2008, 'A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis', Journal of Microbiological Methods, vol. 73, no. 3, pp. 269-272. https://doi.org/10.1016/j.mimet.2007.11.014
Kenefic, L. J. ; Beaudry, J. ; Trim, C. ; Huynh, L. ; Zanecki, S. ; Matthews, M. ; Schupp, J. ; Van Ert, M. ; Keim, Paul S. / A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis. In: Journal of Microbiological Methods. 2008 ; Vol. 73, No. 3. pp. 269-272.
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