A high-density soybean genetic map based on AFLP markers

Paul S Keim, James M. Schupp, Steven E. Travis, Kathryn Clayton, Tong Zhu, Liang Shi, Arnaldo Ferreira, David M. Webb

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Genetic marker systems have improved dramatically in the past 10 yr. Each new system needs to be evaluated for its distribution of markers on genetic linkage maps to validate its use for genetic analysis. The resulting maps are also useful for establishing the genetic positions of genes affecting important phenotypes. We have constructed a high density map in soybean [Glycine max (L.) Merr.] using a 300 RIL (recombinant inbred line) population from BSR-101 x PI437.654 by first constructing an RFLP (restriction fragment length polymorphism) 'scaffold' map based on the entire population. The RFLP anchored map was then further populated with AFLP (amplified fragment length polymorphism) markers based on only a 42 RIL subset. We report here an 840 marker map consisting of 165 RFLP, 25 RAPD (random amplified polymorphic DNA), and 650 AFLP markers spread over 28 linkage groups representing 3441 cM distance. Although clustering of AFLP markers occurred, markers were mapped to every linkage group and were well distributed relative to other marker systems. The AFLP marker system appears to be a useful approach for generating high density genetic maps in soybean.

Original languageEnglish (US)
Pages (from-to)537-543
Number of pages7
JournalCrop Science
Volume37
Issue number2
StatePublished - Mar 1997

Fingerprint

amplified fragment length polymorphism
soybeans
restriction fragment length polymorphism
linkage groups
inbred lines
random amplified polymorphic DNA technique
chromosome mapping
genetic techniques and protocols
Glycine max
phenotype
genetic markers
genes

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Keim, P. S., Schupp, J. M., Travis, S. E., Clayton, K., Zhu, T., Shi, L., ... Webb, D. M. (1997). A high-density soybean genetic map based on AFLP markers. Crop Science, 37(2), 537-543.

A high-density soybean genetic map based on AFLP markers. / Keim, Paul S; Schupp, James M.; Travis, Steven E.; Clayton, Kathryn; Zhu, Tong; Shi, Liang; Ferreira, Arnaldo; Webb, David M.

In: Crop Science, Vol. 37, No. 2, 03.1997, p. 537-543.

Research output: Contribution to journalArticle

Keim, PS, Schupp, JM, Travis, SE, Clayton, K, Zhu, T, Shi, L, Ferreira, A & Webb, DM 1997, 'A high-density soybean genetic map based on AFLP markers', Crop Science, vol. 37, no. 2, pp. 537-543.
Keim PS, Schupp JM, Travis SE, Clayton K, Zhu T, Shi L et al. A high-density soybean genetic map based on AFLP markers. Crop Science. 1997 Mar;37(2):537-543.
Keim, Paul S ; Schupp, James M. ; Travis, Steven E. ; Clayton, Kathryn ; Zhu, Tong ; Shi, Liang ; Ferreira, Arnaldo ; Webb, David M. / A high-density soybean genetic map based on AFLP markers. In: Crop Science. 1997 ; Vol. 37, No. 2. pp. 537-543.
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